LDCA

Name: LDCA
Brand: MedChemExpress
SKU: HY-147361
Rating: 4.5 (1 reviews)

Cat. No.: HY-147361 Purity: 99.18%: FAQs
Data Sheet SDS COA Handling Instructions

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LDCA is a dual-hit metabolic modulator and inhibits LDH-A enzyme activity to stimulate apoptosis in the malignant population. LDCA can be used for the research of oncogenic progression.

For research use only. We do not sell to patients.

LDCA Chemical Structure

CAS No. : 349106-80-5

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

LDCA is a dual-hit metabolic modulator and inhibits LDH-A enzyme activity to stimulate apoptosis in the malignant population. LDCA can be used for the research of oncogenic progression^[1].

In Vitro

LDCA (2-100 μM; 16-72 h) is used in combination with doxorubicin synergistically, enhances the growth inhibition and induces mitochondria-mediated apoptosis by recruiting the caspase cascade, restricting migration, and obviating the clonogenic outgrowth potential of melanoma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	B16-F10 cells
Concentration:	2-100 μM
Incubation Time:	72 h
Result:	Arrested cell growth with a dose-dependent cytotoxic effect and had a strong synergism with LDCA.

Apoptosis Analysis^[1]

Cell Line:	B16-F10 cells
Concentration:	20 μM
Incubation Time:	24 h
Result:	Resulted 15% death when cells exposed to LDCA and caused 40% melanoma cell death combination synergistically with doxorubicin.

Immunofluorescence^[1]

Cell Line:	B16-F10 cells
Concentration:	20 μM
Incubation Time:	16 h
Result:	Demonstrated that combination with doxorubicin resultantly affected cellular morphology with condensed and fragmented nuclei.

Cell Migration Assay ^[1]

Cell Line:	B16-F10 cells
Concentration:	20 μM
Incubation Time:	16 h
Result:	Signicantly limited the migratory potential in the B16-F10 cells.

In Vivo

LDCA (2 mg/kg, iv., the 6th day, once) is used in combination with doxorubicin thwarts tumor growth kinetics to restrain oncogenic progression, thus accentuating survival in the model of murine melanoma^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	melanoma tumor model^[1]
Dosage:	2 mg/kg
Administration:	2 mg/kg, iv., the 6th day, once
Result:	Significantly increased mice survivability combination with doxorubicin, and relieved mice tumor necrosis phenomena.

Molecular Weight

256.49

Formula

C₈H₅Cl₃FNO

CAS No.

349106-80-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

FC1=C(Cl)C=C(NC(C(Cl)Cl)=O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (389.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8988 mL	19.4939 mL	38.9879 mL
5 mM	0.7798 mL	3.8988 mL	7.7976 mL
10 mM	0.3899 mL	1.9494 mL	3.8988 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.18%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (246 KB) HNMR (238 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Saha, Suchandrima, et al. The dual-hit metabolic modulator LDCA synergistically potentiates doxorubicin to selectively combat cancer-associated hallmarks. RSC Advances, 7(84), 53322–53333. doi:10.1039/c7ra08625c.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8988 mL	19.4939 mL	38.9879 mL	97.4697 mL
	5 mM	0.7798 mL	3.8988 mL	7.7976 mL	19.4939 mL
	10 mM	0.3899 mL	1.9494 mL	3.8988 mL	9.7470 mL
	15 mM	0.2599 mL	1.2996 mL	2.5992 mL	6.4980 mL
	20 mM	0.1949 mL	0.9747 mL	1.9494 mL	4.8735 mL
	25 mM	0.1560 mL	0.7798 mL	1.5595 mL	3.8988 mL
	30 mM	0.1300 mL	0.6498 mL	1.2996 mL	3.2490 mL
	40 mM	0.0975 mL	0.4873 mL	0.9747 mL	2.4367 mL
	50 mM	0.0780 mL	0.3899 mL	0.7798 mL	1.9494 mL
	60 mM	0.0650 mL	0.3249 mL	0.6498 mL	1.6245 mL
	80 mM	0.0487 mL	0.2437 mL	0.4873 mL	1.2184 mL
	100 mM	0.0390 mL	0.1949 mL	0.3899 mL	0.9747 mL

LDCA Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LDCA349106-80-5ApoptosisLactate DehydrogenaseLDHdual-hit metabolic modulatorLDH-AapoptosiscancerInhibitorinhibitorinhibit

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LDCA

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Complete Stock Solution Preparation Table

LDCA Related Classifications

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