2. Autophagy Apoptosis
  3. Autophagy Mitophagy Apoptosis
  4. Liensinine

Liensinine is an autophagy/mitophagy inhibitor. Liensinine, a major isoquinoline alkaloid, extracted from the seed embryo of Nelumbo nucifera Gaertn, has a wide range of biological activities, including anti-arrhythmias, anti-hypertension, anti-pulmonary fibrosis, relaxation on vascular smooth muscle, etc.

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Liensinine Chemical Structure

Liensinine Chemical Structure

CAS No. : 2586-96-1

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10 mM * 1 mL in DMSO
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Based on 10 publication(s) in Google Scholar

Other Forms of Liensinine:

Liensinine Related Antibodies

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Description

Liensinine is an autophagy/mitophagy inhibitor. Liensinine, a major isoquinoline alkaloid, extracted from the seed embryo of Nelumbo nucifera Gaertn, has a wide range of biological activities, including anti-arrhythmias, anti-hypertension, anti-pulmonary fibrosis, relaxation on vascular smooth muscle, etc[1].

In Vivo

Liensinine (oral gavage, 100 or 200 mg/kg, daily, 10 weeks) can effectively treat periodontitis, similar to metronidazole-type drugs, and has a certain dose-dependent[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: KM mice[2]
Dosage: 100 mg/kg, 200 mg/kg
Administration: oral gavage, daily, 10 weeks
Result: Reduced the gingival index and increased the SOD levels, the CAT, GSH-Px levels and decreased the NO, MDA, and ET levels compared to the control group.
Animal Model: Institute of Cancer Research (ICR) mice (male, 20-22 g)[3]
Dosage:
Administration: 5 mg/kg by oral administration; 1 mg/kg by intravenous administration
Result: The pharmacokinetic parameters of Liensinine in mice
Parameters po (5 mg/kg) iv (1 mg/kg)
AUC(0-t) (ng/mL*h) 18.8 ± 2.7 211.2 ± 54.9
AUC(0-∞) (ng/mL*h) 19.1 ± 2.8 227.9 ± 60.1
MRT(0-t)( h) 3.2 ± 0.4 2.6 ± 0.5
MRT(0-∞) (h) 3.4 ± 0.5 3.5 ± 0.9
t1/2z (h) 1.9 ± 0.2 3.8 ± 0.8
CLz/F (L/h/kg) 266.0 ± 41.3 4.7 ± 1.2
Vz/F (L/kg) 708.5 ± 79.9 25.9 ± 11.0
Cmax (ng/mL) 5.3 ± 0.2 169.5 ± 53.5
Clinical Trial
Molecular Weight

610.74

Formula

C37H42N2O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(C[C@H]2N(C)CCC3=C2C=C(OC)C(OC)=C3)C=C1OC4=CC5=C(C=C4OC)CCN(C)[C@@H]5CC6=CC=C(O)C=C6
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (81.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6374 mL 8.1868 mL 16.3736 mL
5 mM 0.3275 mL 1.6374 mL 3.2747 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 20 mg/mL (32.75 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.97%

SDS (479 KB)

COA (250 KB) HNMR (280 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6374 mL 8.1868 mL 16.3736 mL 40.9339 mL
5 mM 0.3275 mL 1.6374 mL 3.2747 mL 8.1868 mL
10 mM 0.1637 mL 0.8187 mL 1.6374 mL 4.0934 mL
15 mM 0.1092 mL 0.5458 mL 1.0916 mL 2.7289 mL
20 mM 0.0819 mL 0.4093 mL 0.8187 mL 2.0467 mL
25 mM 0.0655 mL 0.3275 mL 0.6549 mL 1.6374 mL
30 mM 0.0546 mL 0.2729 mL 0.5458 mL 1.3645 mL
40 mM 0.0409 mL 0.2047 mL 0.4093 mL 1.0233 mL
50 mM 0.0327 mL 0.1637 mL 0.3275 mL 0.8187 mL
60 mM 0.0273 mL 0.1364 mL 0.2729 mL 0.6822 mL
80 mM 0.0205 mL 0.1023 mL 0.2047 mL 0.5117 mL
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Liensinine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Liensinine2586-96-1AutophagyMitophagyApoptosisMitochondrial AutophagyapoptosisautophagyautophagicstressbreastcancerDNM1LlysosomemitochondrialfissionRAB7AInhibitorinhibitorinhibit

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