LLL3 (Synonyms: CLT-005)

Name: LLL3
Brand: MedChemExpress
SKU: HY-124843
Rating: 4.5 (1 reviews)

Cat. No.: HY-124843: FAQs
Data Sheet Handling Instructions Technical Support

LLL3 (CLT-005) is a STAT3 inhibitor. LLL3 inhibits dimerization and phosphorylation of STAT3, thereby preventing intraconuclear transfer of STAT3 and inhibits the expression of STAT3 dependent genes, which encode proteins such as Bcl-xL and cyclin D1. In addition, LLL3 can induce cell growth inhibition and apoptosis in human breast cancer and rhabdomyosarcoma cells via the caspase pathway. LLL3 can be used in the study of STAT3 persistent activation types of cancer.

For research use only. We do not sell to patients.

LLL3 Chemical Structure

CAS No. : 63972-38-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	Get quote	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Get quote	Estimated Time of Arrival: December 31
Solid
5 mg	Get quote 6 - 8 weeks 6 - 8 weeks 5 - 7 weeks	Estimated Time of Arrival: December 31
10 mg	Get quote 6 - 8 weeks 6 - 8 weeks 5 - 7 weeks	Estimated Time of Arrival: December 31
25 mg	Get quote 6 - 8 weeks 6 - 8 weeks 5 - 7 weeks	Estimated Time of Arrival: December 31
50 mg	Get quote 6 - 8 weeks 6 - 8 weeks 5 - 7 weeks	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

Synthetic products have potential research and development risk.

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All STAT Isoform Specific Products:

View All Isoforms

STAT3 STAT5 STAT6 STAT1

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	IC₅₀	11.5 μM Compound: LLL3	Antiproliferative activity against human DU145 cells assessed as growth inhibition after 72 hrs by MTS assay Antiproliferative activity against human DU145 cells assessed as growth inhibition after 72 hrs by MTS assay	[PMID: 24176397]
DU-145	IC₅₀	16.2 μM Compound: 1	Antiproliferative activity against human DU145 cells after 72 hrs by MTS assay Antiproliferative activity against human DU145 cells after 72 hrs by MTS assay	[PMID: 18006313]
HT-29	IC₅₀	10.4 μM Compound: LLL3	Antiproliferative activity against human HT-29 cells assessed as growth inhibition after 72 hrs by MTS assay Antiproliferative activity against human HT-29 cells assessed as growth inhibition after 72 hrs by MTS assay	[PMID: 24176397]
LNCaP	IC₅₀	34.1 μM Compound: 1	Antiproliferative activity against human LNCaP cells after 72 hrs by MTS assay Antiproliferative activity against human LNCaP cells after 72 hrs by MTS assay	[PMID: 18006313]
MCF7	IC₅₀	88.5 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay	[PMID: 18006313]
PC-3	IC₅₀	13.4 μM Compound: 1	Antiproliferative activity against human PC3 cells after 72 hrs by MTS assay Antiproliferative activity against human PC3 cells after 72 hrs by MTS assay	[PMID: 18006313]

Molecular Weight

266.25

Formula

C₁₆H₁₀O₄

CAS No.

63972-38-3

SMILES

O=C1C2=C(C(O)=CC=C2)C(C3=CC=CC(C(C)=O)=C13)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (75.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7559 mL	18.7793 mL	37.5587 mL
5 mM	0.7512 mL	3.7559 mL	7.5117 mL
10 mM	0.3756 mL	1.8779 mL	3.7559 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (7.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Fuh B, et al. LLL-3 inhibits STAT3 activity, suppresses glioblastoma cell growth and prolongs survival in a mouse glioblastoma model[J]. British journal of cancer, 2009, 100(1): 106-112. [Content Brief]

[2]. Lavecchia A, et al. Novel inhibitors of signal transducer and activator of transcription 3 signaling pathway: an update on the recent patent literature[J]. Expert opinion on therapeutic patents, 2014, 24(4): 383-400. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7559 mL	18.7793 mL	37.5587 mL	93.8967 mL
	5 mM	0.7512 mL	3.7559 mL	7.5117 mL	18.7793 mL
	10 mM	0.3756 mL	1.8779 mL	3.7559 mL	9.3897 mL
	15 mM	0.2504 mL	1.2520 mL	2.5039 mL	6.2598 mL
	20 mM	0.1878 mL	0.9390 mL	1.8779 mL	4.6948 mL
	25 mM	0.1502 mL	0.7512 mL	1.5023 mL	3.7559 mL
	30 mM	0.1252 mL	0.6260 mL	1.2520 mL	3.1299 mL
	40 mM	0.0939 mL	0.4695 mL	0.9390 mL	2.3474 mL
	50 mM	0.0751 mL	0.3756 mL	0.7512 mL	1.8779 mL
	60 mM	0.0626 mL	0.3130 mL	0.6260 mL	1.5649 mL

LLL3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LLL363972-38-3CLT-005LLL 3LLL-3CLT005CLT 005STATApoptosisSTAT3dimerizationphosphorylationinhibitorapoptosisInhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

LLL3 (Synonyms: CLT-005)

Customer Review

LLL3 Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All STAT Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

LLL3 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report