LOX-IN-3 dihydrochloride

Name: LOX-IN-3 dihydrochloride
Brand: MedChemExpress
SKU: HY-138625A
Rating: 4.5 (1 reviews)

Cat. No.: HY-138625A Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

LOX-IN-3 dihydrochloride is an orally active lysyl oxidase (LOX) inhibitor. LOX-IN-3 dihydrochloride can be used for fibrosis, cancer and angiogenesis research.

For research use only. We do not sell to patients.

LOX-IN-3 dihydrochloride Chemical Structure

CAS No. : 2409964-23-2

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of LOX-IN-3 dihydrochloride:

LOX-IN-3 Get quote
LOX-IN-3 dihydrochloride monohydrate Get quote

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•Related Screening Libraries:

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MAO-A MAO-B

Biological Activity
Purity & Documentation
References
Customer Review

Description

LOX-IN-3 dihydrochloride is an orally active lysyl oxidase (LOX) inhibitor. LOX-IN-3 dihydrochloride can be used for fibrosis, cancer and angiogenesis research^[1].

IC₅₀ & Target

IC₅₀: <1 μM (human LOXL2), <10 μM (bovine LOX)^[1]

In Vitro

LOX-IN-3 dihydrochloride monohydrate (Compound 33) inhibits the bovine LOX and human LOXL2 activities with IC₅₀ values of <10 μM and <1 μM, respectively^[1].
LOX-IN-3 dihydrochloride monohydrate exhibits sustained inhibition of LOXL1 and LOXL2^[1].
LOX-IN-3 dihydrochloride monohydrate is less active against SSAO/VAP-1 and MAO-B activities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

LOX-IN-3 dihydrochloride monohydrate (Compound 33) (30 mg/kg; orally; once) inhibits lysyl oxidase activity in rats^[1].
LOX-IN-3 dihydrochloride monohydrate (10 mg/kg; orally; daily for 14 days) reduces kidney fibrosis in unilateral ureteric obstruction (UUO) mice model^[1].
LOX-IN-3 dihydrochloride monohydrate (15 mg/kg; orally; daily for 21 days) reduces lung fibrosis in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats^[1]
Dosage:	30 mg/kg
Administration:	Oral administration, single dose
Result:	Completely abolished lysyl oxidase activity. Plasma concentrations of tested compound are far below the IC₅₀ after 8 hours, the half-life of recovery is between 2-3 days (ear) and 24 hours (aorta).

Animal Model:	Unilateral ureteric obstruction (UUO) model of acute kidney fibrosis in mice^[1]
Dosage:	10 mg/kg
Administration:	Oral gavage, daily for 14 days
Result:	Increased kidney weight and thickness and reduced the area of fibrosis.

Animal Model:	C57Bl/6 mice, Bleomycin-induced lung fibrosis model
Dosage:	15 mg/kg
Administration:	Oral gavage, daily for 21 days
Result:	Significantly reduced the Ashcroft score and the lung weight.

Molecular Weight

353.24

Formula

C₁₃H₁₅Cl₂FN₂O₂S

CAS No.

2409964-23-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

NC/C=C(F)/CS(=O)(C1=C2N=CC=CC2=CC=C1)=O.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (283.09 mM)

DMSO : 33.33 mg/mL (94.36 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8309 mL	14.1547 mL	28.3094 mL
5 mM	0.5662 mL	2.8309 mL	5.6619 mL
10 mM	0.2831 mL	1.4155 mL	2.8309 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (251 KB) HNMR (269 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Alison Dorothy Findlay, et al. Haloallylamine sulfone derivative inhibitors of lysyl oxidases and uses thereof. WO2020024017A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.8309 mL	14.1547 mL	28.3094 mL	70.7734 mL
	5 mM	0.5662 mL	2.8309 mL	5.6619 mL	14.1547 mL
	10 mM	0.2831 mL	1.4155 mL	2.8309 mL	7.0773 mL
	15 mM	0.1887 mL	0.9436 mL	1.8873 mL	4.7182 mL
	20 mM	0.1415 mL	0.7077 mL	1.4155 mL	3.5387 mL
	25 mM	0.1132 mL	0.5662 mL	1.1324 mL	2.8309 mL
	30 mM	0.0944 mL	0.4718 mL	0.9436 mL	2.3591 mL
	40 mM	0.0708 mL	0.3539 mL	0.7077 mL	1.7693 mL
	50 mM	0.0566 mL	0.2831 mL	0.5662 mL	1.4155 mL
	60 mM	0.0472 mL	0.2359 mL	0.4718 mL	1.1796 mL
	80 mM	0.0354 mL	0.1769 mL	0.3539 mL	0.8847 mL
H₂O	100 mM	0.0283 mL	0.1415 mL	0.2831 mL	0.7077 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.