2. Epigenetics Immunology/Inflammation
  3. Histone Demethylase PD-1/PD-L1
  4. LSD1-IN-24

LSD1-IN-24(compound 3S) is a selective LSD1 inhibitor with IC50 = 0.247 μM. LSD1-IN-24 can mediate the expression of PD-L1, enhance T cell killing response, and can be used in cancer research.

For research use only. We do not sell to patients.

LSD1-IN-24 Chemical Structure

LSD1-IN-24 Chemical Structure

CAS No. : 4734-59-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 165 In-stock
Solution
10 mM * 1 mL in DMSO USD 165 In-stock
Solid
5 mg USD 150 In-stock
10 mg USD 244 In-stock
25 mg USD 488 In-stock
50 mg USD 780 In-stock
100 mg USD 1250 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

LSD1-IN-24 Related Antibodies

Top Publications Citing Use of Products

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KDM1/LSD1 KDM2 KDM3 KDM4 KDM5 KDM6 KDM7

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

LSD1-IN-24(compound 3S) is a selective LSD1 inhibitor with IC50 = 0.247 μM. LSD1-IN-24 can mediate the expression of PD-L1, enhance T cell killing response, and can be used in cancer research[1].

In Vitro

LSD1-IN-24 (compound 3S)(0-20 μM, 5 days) can reduce PD-L1 levels in an LSD1-dependent manner and enhance the T cell killing response of BGC-823 cells in a dose-dependent manner, but does not affect BGC -823 and MFC cell proliferation. Furthermore, it dose-dependently protects H3K4me1/2 but not H3K4me3 from demethylation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LSD1-IN-24 Related Antibodies
In Vivo

LSD1-IN-24 (compound 3S) (0-50 mg/kg, oral gavage; daily for 2 weeks) inhibits in vivo MFC cell growth, tumor weight in a dose-dependent manner in a subcutaneous tumor model containing MFC cells Significantly decreased, tumor tissue Ki67 decreased significantly, and no obvious toxic effect on mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male 615 mice with MFC cell[1]
Dosage: 0, 10, 25, and 50 mg/kg
Administration: Oral gavage; daily for 2 weeks
Result: Significantly decreased intratumoral PD-L1 expression, increased CD3+, CD4+, CD8+ cell numbers, interleukin-2 (IL2) and IFNγ mRNA and protein levels were also upregulated.
Molecular Weight

312.43

Formula

C18H20N2OS
CAS No.
Appearance

Solid

Color

Pale purple to purple

SMILES

N1(CCN2C3=C(SC4=C2C=CC=C4)C=CC=C3)CCOCC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (320.07 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2007 mL 16.0036 mL 32.0072 mL
5 mM 0.6401 mL 3.2007 mL 6.4014 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (8.00 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2007 mL 16.0036 mL 32.0072 mL 80.0179 mL
5 mM 0.6401 mL 3.2007 mL 6.4014 mL 16.0036 mL
10 mM 0.3201 mL 1.6004 mL 3.2007 mL 8.0018 mL
15 mM 0.2134 mL 1.0669 mL 2.1338 mL 5.3345 mL
20 mM 0.1600 mL 0.8002 mL 1.6004 mL 4.0009 mL
25 mM 0.1280 mL 0.6401 mL 1.2803 mL 3.2007 mL
30 mM 0.1067 mL 0.5335 mL 1.0669 mL 2.6673 mL
40 mM 0.0800 mL 0.4001 mL 0.8002 mL 2.0004 mL
50 mM 0.0640 mL 0.3201 mL 0.6401 mL 1.6004 mL
60 mM 0.0533 mL 0.2667 mL 0.5335 mL 1.3336 mL
80 mM 0.0400 mL 0.2000 mL 0.4001 mL 1.0002 mL
100 mM 0.0320 mL 0.1600 mL 0.3201 mL 0.8002 mL
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LSD1-IN-24 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LSD1-IN-244734-59-2Histone DemethylasePD-1/PD-L1PD-1/Programmed death-ligand 1PD-L1LSD1cancerT cellInhibitorinhibitorinhibit

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