McN-A-343

Name: McN-A-343
Brand: MedChemExpress
SKU: HY-107648
Rating: 5.0 (2 reviews)

Cat. No.: HY-107648 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

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McN-A-343 is a selective M1 muscarinic agonist that stimulates muscarinic transmission in sympathetic ganglia. McN-A-343 reduces inflammation and oxidative stress in an experimental model of ulcerative colitis. McN-A-343 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

McN-A-343 Chemical Structure

CAS No. : 55-45-8

Size	Price	Stock	Quantity
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE McN-A-343

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Description

McN-A-343 is a selective M1 muscarinic agonist that stimulates muscarinic transmission in sympathetic ganglia. McN-A-343 reduces inflammation and oxidative stress in an experimental model of ulcerative colitis^[1]^[2]. McN-A-343 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

mAChR1

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	12.93 μM Compound: McN-A-343	Inhibitory activity against human Muscarinic acetylcholine receptor M2 using [3H]quinuclidinyl benzilate to label antagonist site (RQNB) in CHO cells Inhibitory activity against human Muscarinic acetylcholine receptor M2 using [3H]quinuclidinyl benzilate to label antagonist site (RQNB) in CHO cells	10.1016/S0960-894X(00)80538-7
CHO	IC₅₀	4 μM Compound: McN-A-343	Inhibitory activity against human Muscarinic acetylcholine receptor M1 using [3H]quinuclidinyl benzilate to label antagonist site (RQNB) in CHO cells Inhibitory activity against human Muscarinic acetylcholine receptor M1 using [3H]quinuclidinyl benzilate to label antagonist site (RQNB) in CHO cells	10.1016/S0960-894X(00)80538-7

Molecular Weight

317.21

Formula

C₁₄H₁₈Cl₂N₂O₂

CAS No.

55-45-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(Cl)=CC=C1)OCC#CC[N+](C)(C)C.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (315.25 mM; Need ultrasonic)

DMSO : 100 mg/mL (315.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1525 mL	15.7624 mL	31.5249 mL
5 mM	0.6305 mL	3.1525 mL	6.3050 mL
10 mM	0.3152 mL	1.5762 mL	3.1525 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (244 KB) HNMR (247 KB) LCMS (248 KB)

Handling Instructions (2659 KB)

References

[1]. Koss MC, et al. Analysis of miosis produced by McN-A-343 in anesthetized cats. J Ocul Pharmacol Ther. 1995;11(3):389-399. [Content Brief]

[2]. Magalhães DA, Batista JA, Sousa SG, et al. McN-A-343, a muscarinic agonist, reduces inflammation and oxidative stress in an experimental model of ulcerative colitis. Life Sci. 2021;272:119194. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1525 mL	15.7624 mL	31.5249 mL	78.8121 mL
	5 mM	0.6305 mL	3.1525 mL	6.3050 mL	15.7624 mL
	10 mM	0.3152 mL	1.5762 mL	3.1525 mL	7.8812 mL
	15 mM	0.2102 mL	1.0508 mL	2.1017 mL	5.2541 mL
	20 mM	0.1576 mL	0.7881 mL	1.5762 mL	3.9406 mL
	25 mM	0.1261 mL	0.6305 mL	1.2610 mL	3.1525 mL
	30 mM	0.1051 mL	0.5254 mL	1.0508 mL	2.6271 mL
	40 mM	0.0788 mL	0.3941 mL	0.7881 mL	1.9703 mL
	50 mM	0.0630 mL	0.3152 mL	0.6305 mL	1.5762 mL
	60 mM	0.0525 mL	0.2627 mL	0.5254 mL	1.3135 mL
	80 mM	0.0394 mL	0.1970 mL	0.3941 mL	0.9852 mL
	100 mM	0.0315 mL	0.1576 mL	0.3152 mL	0.7881 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

McN-A-343 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

McN-A-34355-45-8mAChRMuscarinic acetylcholine receptorM1 muscarinicmuscarinic transmissionsympathetic gangliainflammationoxidative stressInhibitorinhibitorinhibit

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McN-A-343

Customer Review

McN-A-343 Related Antibodies

2 Publications Citing Use of MCE McN-A-343

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

Biological Activity

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References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

McN-A-343 Related Classifications

Keywords:

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SAFETY DATA SHEET (SDS)

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