MCT1-IN-2

Name: MCT1-IN-2
Brand: MedChemExpress
SKU: HY-18974
Rating: 4.5 (1 reviews)

Cat. No.: HY-18974 Purity: 99.16%: FAQs
Data Sheet SDS COA Handling Instructions

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MCT1-IN-2 is a potent monocarboxylate transporter 1 (MCT1) inhibitor. MCT1-IN-2 has anti-cancer activity.

For research use only. We do not sell to patients.

MCT1-IN-2 Chemical Structure

CAS No. : 227321-12-2

Size	Price	Stock	Quantity
5 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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MCT1 MCT2 MCT4

Biological Activity
Purity & Documentation
References
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Description

MCT1-IN-2 is a potent monocarboxylate transporter 1 (MCT1) inhibitor. MCT1-IN-2 has anti-cancer activity^[1]^[2].

IC₅₀ & Target

MCT1

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	EC₅₀	> 10 μM Compound: 1	Growth inhibition of human MCF7 cells overexpressing MCT4 after 4 days by MTT assay Growth inhibition of human MCF7 cells overexpressing MCT4 after 4 days by MTT assay	[PMID: 25068893]
MCF7	IC₅₀	105 nM Compound: 1	Inhibition of MCT1 (unknown origin) overexpressed in human MCF7 cells assessed as inhibition of transporter-mediated [14C]lactate transport after 10 mins by scintillation counting Inhibition of MCT1 (unknown origin) overexpressed in human MCF7 cells assessed as inhibition of transporter-mediated [14C]lactate transport after 10 mins by scintillation counting	[PMID: 25068893]
MCF7	EC₅₀	21 nM Compound: 1	Growth inhibition of human MCF7 cells overexpressing MCT1 after 4 days by MTT assay Growth inhibition of human MCF7 cells overexpressing MCT1 after 4 days by MTT assay	[PMID: 25068893]
Raji	EC₅₀	0.5 nM Compound: 1	Growth inhibition of human Raji cells after 96 hrs by MTT assay Growth inhibition of human Raji cells after 96 hrs by MTT assay	[PMID: 25068893]
Raji	EC₅₀	5 nM Compound: 5	Inhibition of MCT1 in human Raji cells assessed as inhibition of cell proliferation after 96 hrs by MTT assay Inhibition of MCT1 in human Raji cells assessed as inhibition of cell proliferation after 96 hrs by MTT assay	[PMID: 27347360]

In Vitro

MCT1-IN-2 (compound 3) has a pK_i of 9.48 for Jurkat T-cells and inhibits human peripheral blood mononuclear cells (PBMC) proliferation (pIC₅₀=8.61)^[1].
MCT1-IN-2 (compound 1) has an EC₅₀ of 5.5 nM (MTT assay) and an IC₅₀ of 105 nM (lactate transport inhibition assay) in human Raji lymphoma cell proliferation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

435.58

Formula

C₂₅H₂₉N₃O₂S

CAS No.

227321-12-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1C2=C(SCCCO)N(CC3=C4C=CC=CC4=CC=C3)C=C2C(CC(C)C)=NN1C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (229.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2958 mL	11.4789 mL	22.9579 mL
5 mM	0.4592 mL	2.2958 mL	4.5916 mL
10 mM	0.2296 mL	1.1479 mL	2.2958 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.16%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (255 KB) HNMR (202 KB) RP-HPLC (238 KB) LCMS (106 KB) Elemental Analysis Report (112 KB)

Handling Instructions (2659 KB)

References

[1]. Clare M Murray, et al. Monocarboxylate transporter MCT1 is a target for immunosuppression. Nat Chem Biol. 2005 Dec;1(7):371-6. [Content Brief]

[2]. Hui Wang, et al. Synthesis and structure-activity relationships of pteridine dione and trione monocarboxylate transporter 1 inhibitors. J Med Chem. 2014 Sep 11;57(17):7317-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2958 mL	11.4789 mL	22.9579 mL	57.3947 mL
	5 mM	0.4592 mL	2.2958 mL	4.5916 mL	11.4789 mL
	10 mM	0.2296 mL	1.1479 mL	2.2958 mL	5.7395 mL
	15 mM	0.1531 mL	0.7653 mL	1.5305 mL	3.8263 mL
	20 mM	0.1148 mL	0.5739 mL	1.1479 mL	2.8697 mL
	25 mM	0.0918 mL	0.4592 mL	0.9183 mL	2.2958 mL
	30 mM	0.0765 mL	0.3826 mL	0.7653 mL	1.9132 mL
	40 mM	0.0574 mL	0.2870 mL	0.5739 mL	1.4349 mL
	50 mM	0.0459 mL	0.2296 mL	0.4592 mL	1.1479 mL
	60 mM	0.0383 mL	0.1913 mL	0.3826 mL	0.9566 mL
	80 mM	0.0287 mL	0.1435 mL	0.2870 mL	0.7174 mL
	100 mM	0.0230 mL	0.1148 mL	0.2296 mL	0.5739 mL

MCT1-IN-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MCT1-IN-2227321-12-2Monocarboxylate TransporterMCT1JurkatT-cellsperipheralbloodmononuclearPBMCRajilymphomaInhibitorinhibitorinhibit

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MCT1-IN-2

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MCT1-IN-2 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All Monocarboxylate Transporter Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

MCT1-IN-2 Related Antibodies

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Complete Stock Solution Preparation Table

MCT1-IN-2 Related Classifications

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