Methdilazine hydrochloride

Name: Methdilazine hydrochloride
Brand: MedChemExpress
SKU: HY-B1690A
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1690A Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Methdilazine hydrochloride is an orally active antibiotic (histamine antagonist). Methdilazine hydrochloride can inhibit various mycobacterium with MIC values at 5-15 μg/mL in vitro and in vivo, which can be used for the research of infectious diseases.

For research use only. We do not sell to patients.

Methdilazine hydrochloride Chemical Structure

CAS No. : 1229-35-2

Size	Price	Stock	Quantity
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Methdilazine hydrochloride:

Methdilazine-d4 hydrochloride Get quote
Methdilazine Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Methdilazine hydrochloride (0-20 μg/mL approximately, 18 h) inhibits kinds of mycobacterium with MIC values ranging from 5 μg/mL to 15 μg/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	M. smegmatis 798/1546, M.,fortuitum 1529, M. scrofulaceum 1323, M. gordonae 1324, M. rnarinum 50, M.,flavescens 1541, M. terrae 1450, M. tuberculosis, H₃₇Ra 16, H₃₇Rv 16, K1, K2, ICRC bacillus,'Skinsnes' bacillus.
Concentration:	0-20 μg/mL approximately
Incubation Time:	18 h
Result:	Inhibited mycobacterium with MIC values ranging from 5 μg/mL to 15 μg/mL.

In Vivo

Methdilazine hydrochloride (Intraperitoneal injection, 10 μg/gm body wt/day, 6 weeks) is antagonistic to mycobacteria in H₃₇Rv infected mice^[1].
Methdilazine hydrochloride (Oral administration, 10 mg/kg per day, 28 days) improves survival of Mycobacterium Tuberculosis (Mtb) H37Rv infected mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	H₃₇Rv infected mice^[1]
Dosage:	10 μg/gm body wt/day, 6 weeks
Administration:	Intraperitoneal injection
Result:	Displayed an anti-mycobacterial activity to mycobacteria.

Animal Model:	Mycobacterium Tuberculosis (Mtb) H₃₇Rv infected Swiss albino male mice^[2]
Dosage:	10 mg/kg per day for 28 days
Administration:	Oral administration
Result:	Increased surviving time to 28 days with no sign of disease, showed 71.42% survival.

Molecular Weight

332.89

Formula

C₁₈H₂₁ClN₂S

CAS No.

1229-35-2

Appearance

Solid

Color

White to off-white

SMILES

CN(CC1)CC1CN2C3=C(C=CC=C3)SC4=C2C=CC=C4.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (150.20 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0040 mL	15.0200 mL	30.0400 mL
5 mM	0.6008 mL	3.0040 mL	6.0080 mL
10 mM	0.3004 mL	1.5020 mL	3.0040 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.75 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.75 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (280 KB) SDS (251 KB)

COA (256 KB)

Handling Instructions (2659 KB)

References

[1]. A N Chakrabarty, et al. Antimycobacterial activity of methdilazine (Md), an antimicrobic phenothiazine. APMIS. 1993 Jun;101(6):449-54. [Content Brief]

[2]. Noton K Dutta, et al. Activity of the phenothiazine methdilazine alone or in combination with isoniazid or streptomycin against Mycobacterium tuberculosis in mice. J Med Microbiol. 2009 Dec;58(Pt 12):1667-1668. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0040 mL	15.0200 mL	30.0400 mL	75.0999 mL
	5 mM	0.6008 mL	3.0040 mL	6.0080 mL	15.0200 mL
	10 mM	0.3004 mL	1.5020 mL	3.0040 mL	7.5100 mL
	15 mM	0.2003 mL	1.0013 mL	2.0027 mL	5.0067 mL
	20 mM	0.1502 mL	0.7510 mL	1.5020 mL	3.7550 mL
	25 mM	0.1202 mL	0.6008 mL	1.2016 mL	3.0040 mL
	30 mM	0.1001 mL	0.5007 mL	1.0013 mL	2.5033 mL
	40 mM	0.0751 mL	0.3755 mL	0.7510 mL	1.8775 mL
	50 mM	0.0601 mL	0.3004 mL	0.6008 mL	1.5020 mL
	60 mM	0.0501 mL	0.2503 mL	0.5007 mL	1.2517 mL
	80 mM	0.0375 mL	0.1877 mL	0.3755 mL	0.9387 mL
	100 mM	0.0300 mL	0.1502 mL	0.3004 mL	0.7510 mL