2. Metabolic Enzyme/Protease Apoptosis
  3. MMP Apoptosis
  4. MMP2-IN-1

MMP2-IN-1 is a moderate potenet MMP2 inhibitor with IC50 of 6.8 µM. MMP2-IN-1 exhibits remarkable antiproliferative activity in certain cancer cells by arresting the cell cycle and inducing apoptosis.

For research use only. We do not sell to patients.

MMP2-IN-1 Chemical Structure

MMP2-IN-1 Chemical Structure

CAS No. : 2764598-01-6

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Customer Review

Based on 1 publication(s) in Google Scholar

MMP2-IN-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MMP2-IN-1

View All MMP Isoform Specific Products:

View All Isoforms
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MMP2-IN-1 is a moderate potenet MMP2 inhibitor with IC50 of 6.8 µM. MMP2-IN-1 exhibits remarkable antiproliferative activity in certain cancer cells by arresting the cell cycle and inducing apoptosis[1].

IC50 & Target[1]

MMP2

6.8 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
A549 IC50
0.11 μM
Compound: 4a
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
[PMID: 35255412]
HeLa IC50
0.18 μM
Compound: 4a
Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
[PMID: 35255412]
HepG2 IC50
> 10 μM
Compound: 4a
Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
[PMID: 35255412]
MDA-MB-231 IC50
0.07 μM
Compound: 4a
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 74 hrs by SRB assay
[PMID: 35255412]
In Vitro

MMP2-IN-1 (compound 4a) (0-10 μM; 74 hours) exhibits IC50 values of 0.07 μM, 0.11 μM, and 0.18 μM against MDA-MB-231, A549, and HeLa cancer cells, respectively, and over 10 μM in Hep 5G cells[1].
MMP2-IN-1 (10 μM; 24 hours) induces cell cycle arrest in the S phase[1].
MMP2-IN-1 (0.01 μM, 0.1 μM, 1 μM and 10 μM; 24 hours) induces a dose-dependent increment in early-and late-stage apoptosis of MDA-MB-231 cells, and increased the early-stage apoptosis percentage from 4.66% to 10.9% at 10 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MMP2-IN-1 Related Antibodies

Cell Proliferation Assay

Cell Line: MDA-MB-231, A549, HeLa and Hep 5G cells[1]
Concentration: 0-10 μM
Incubation Time: 74 hours
Result: Exhibited IC50 values of 0.07 μM, 0.11 μM, and 0.18 μM against MDA-MB-231, A549, and HeLa cancer cells, respectively, and over 10 μM in Hep 5G cells.

Cell Cycle Analysis

Cell Line: MDA-MB-231[1]
Concentration: 10 μM
Incubation Time: 24 hours
Result: Induced cell cycle arrest in the S phase.

Apoptosis Analysis

Cell Line: MDA-MB-231[1]
Concentration: 0.01 μM, 0.1 μM, 1 μM and 10 μM
Incubation Time: 24 hours
Result: Induced a dose-dependent increment in early-and late-stage apoptosis of MDA-MB-231 cells, and increased the early-stage apoptosis percentage from 4.66% to 10.9% at 10 μM.
In Vivo

MMP2-IN-1 (100 mg/kg, 150 mg/kg, 200 mg/kg, 250 mg/kg; IP, single) causes 0%, 30%, 50% and 60% mortality rate at dosing 100 mg/kg, 150 mg/kg, 200 mg/kg and 250 mg/kg respectively[1].
MMP2-IN-1 (10 mg/kg; IP, daily, for 14 days) significantly inhibits tumor growth in metastatic 4T1 murine breast cancer model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice (n = 10, half male and half female)[1]
Dosage: 100 mg/kg, 150 mg/kg, 200 mg/kg, 250 mg/kg
Administration: IP, single
Result: No mortality occurred after administration of 100 mg/kg, and the mortality rate was 30%, 50% and 60% at dosing 150 mg/kg, 200 mg/kg, 250 mg/kg respectively.
Animal Model: Orthotopic 4T1 tumor-bearing mice[1]
Dosage: 10 mg/kg
Administration: IP, daily, for 14 days
Result: Significantly inhibited tumor growth in metastatic 4T1 murine breast cancer model.
Molecular Weight

319.33

Formula

C15H13NO5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1COC2(C=CC(C=C2)=O)N1S(=O)(C3=CC=C(C)C=C3)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (313.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1316 mL 15.6578 mL 31.3156 mL
5 mM 0.6263 mL 3.1316 mL 6.2631 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (7.83 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (7.83 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1316 mL 15.6578 mL 31.3156 mL 78.2889 mL
5 mM 0.6263 mL 3.1316 mL 6.2631 mL 15.6578 mL
10 mM 0.3132 mL 1.5658 mL 3.1316 mL 7.8289 mL
15 mM 0.2088 mL 1.0439 mL 2.0877 mL 5.2193 mL
20 mM 0.1566 mL 0.7829 mL 1.5658 mL 3.9144 mL
25 mM 0.1253 mL 0.6263 mL 1.2526 mL 3.1316 mL
30 mM 0.1044 mL 0.5219 mL 1.0439 mL 2.6096 mL
40 mM 0.0783 mL 0.3914 mL 0.7829 mL 1.9572 mL
50 mM 0.0626 mL 0.3132 mL 0.6263 mL 1.5658 mL
60 mM 0.0522 mL 0.2610 mL 0.5219 mL 1.3048 mL
80 mM 0.0391 mL 0.1957 mL 0.3914 mL 0.9786 mL
100 mM 0.0313 mL 0.1566 mL 0.3132 mL 0.7829 mL
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MMP2-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MMP2-IN-12764598-01-6MMPApoptosisMatrix metalloproteinasesAnticancerCell proliferationCell cycleMDA-MB-231Kunming miceInhibitorinhibitorinhibit

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