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  5. N-Nitrosomorpholine

N-Nitrosomorpholine is a photosensitive nitrosamine that is carcinogenic and can be used to construct animal cancer models.

For research use only. We do not sell to patients.

N-Nitrosomorpholine Chemical Structure

N-Nitrosomorpholine Chemical Structure

CAS No. : 59-89-2

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Based on 1 publication(s) in Google Scholar

Other Forms of N-Nitrosomorpholine:

N-Nitrosomorpholine Related Antibodies

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Description

N-Nitrosomorpholine is a photosensitive nitrosamine that is carcinogenic and can be used to construct animal cancer models[1].

In Vivo

N-Nitrosomorpholine can be used in animal modeling to create tumor models.
Induction of liver disease[1][2]
Background
High doses of N-Nitrosomorpholine can cause livers to show significant cytoplasmic and nuclear changes, leading to toxic damage. N-Nitrosomorpholine toxicity causes single cell necrosis, loss of glycogen in the gland center; and induces giant cell production, bile duct proliferation and fibrosis. At the 4th week of N-Nitrosomorpholine treatment, cirrhosis will be obvious, accompanied by bile duct fibrosis, cholangiomas and multiple hepatocellular nodules. At weeks 15 and 20, cirrhosis worsens. At weeks 20 to 25, a large number of nodules continue to be induced. At weeks 30 to 40, metastatic liver cancer appears and causes death in animals[3].
Specific Modeling Methods
Mice[1]: B6C3FI hybrid • male • 11 weeks of age • average weight of 27 g
Administration: 160 mg/L • mixed in drinking water • daily for 80 weeks
Rat[2]: Sprague Dawley• male• weighing about 200 g
Administration: 200 mg/L• mixed in drinking water• daily for 80 weeks
Note
(1) Before the experiment, the rats were kept under constant conditions of 22°C, light-dark cycle for 12 hours and received Altromin granule diet and free tap water.
(2) After killing the rats with a rapid spinal dislocation, Livers were quickly extirpated and pieces of about 1 cm x 0.5 cm from the right and left liver lobe were frozen in liquid nitrogen-cooled isopentane and stored at -80 °C.
Modeling Indicators
Molecular changes: serum glutamate-oxaloacetate aminotransferase level ↑; focal glycogen hyperplasia ↑.
Histological analysis: liver damage with loss of lobular architecture and large necrotic areas. Necrotic areas are surrounded by "giant cells" (hepatocytes with irregular size and shape, enlarged nuclei and eosinophilic inclusions) and inflammatory cells. Regenerative nodules appear.
Pathological changes: G6P enzyme-negative and -positive lesions appear, and the average diameter of G6P enzyme-negative and -positive lesions increases.
Opposite Product(s): 3,4,3',4'-tetrachlorobiphenyl

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

116.12

Formula

C4H8N2O2
CAS No.
Appearance

<29°C Solid,>29°C Liquid

Color

Light yellow to yellow

SMILES

O=NN1CCOCC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (861.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.6118 mL 43.0589 mL 86.1178 mL
5 mM 1.7224 mL 8.6118 mL 17.2236 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.91%

SDS (643 KB)

COA (247 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 8.6118 mL 43.0589 mL 86.1178 mL 215.2945 mL
5 mM 1.7224 mL 8.6118 mL 17.2236 mL 43.0589 mL
10 mM 0.8612 mL 4.3059 mL 8.6118 mL 21.5295 mL
15 mM 0.5741 mL 2.8706 mL 5.7412 mL 14.3530 mL
20 mM 0.4306 mL 2.1529 mL 4.3059 mL 10.7647 mL
25 mM 0.3445 mL 1.7224 mL 3.4447 mL 8.6118 mL
30 mM 0.2871 mL 1.4353 mL 2.8706 mL 7.1765 mL
40 mM 0.2153 mL 1.0765 mL 2.1529 mL 5.3824 mL
50 mM 0.1722 mL 0.8612 mL 1.7224 mL 4.3059 mL
60 mM 0.1435 mL 0.7176 mL 1.4353 mL 3.5882 mL
80 mM 0.1076 mL 0.5382 mL 1.0765 mL 2.6912 mL
100 mM 0.0861 mL 0.4306 mL 0.8612 mL 2.1529 mL
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N-Nitrosomorpholine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Nitrosomorpholine59-89-2DNA/RNA SynthesisInhibitorinhibitorinhibit

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