N-Nitrosomorpholine

Name: N-Nitrosomorpholine
Brand: MedChemExpress
SKU: HY-131123
Rating: 4.5 (1 reviews)

Cat. No.: HY-131123 Purity: 99.91%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

N-Nitrosomorpholine is a nitrosamine that is sensitive to light. N-nitrosomorpholine is a strong animal carcinogen.

For research use only. We do not sell to patients.

N-Nitrosomorpholine Chemical Structure

CAS No. : 59-89-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	In-stock	Estimated Time of Arrival: April 2
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: April 2
Solid or liquid
250 mg	USD 25	In-stock	Estimated Time of Arrival: April 2
500 mg	USD 40	In-stock	Estimated Time of Arrival: April 2
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of N-Nitrosomorpholine:

N-Nitrosomorpholine-d₄ Get quote
N-Nitrosomorpholine-d₈ Get quote
N-Nitrosomorpholine (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

N-Nitrosomorpholine is a nitrosamine that is sensitive to light. N-nitrosomorpholine is a strong animal carcinogen^[1].

In Vivo

N-Nitrosomorpholine can be used in animal modeling to construct tumor models.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

116.12

Formula

C₄H₈N₂O₂

CAS No.

59-89-2

Appearance

<29°C Solid,>29°C Liquid

Color

Light yellow to yellow

SMILES

O=NN1CCOCC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (861.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.6118 mL	43.0589 mL	86.1178 mL
5 mM	1.7224 mL	8.6118 mL	17.2236 mL
10 mM	0.8612 mL	4.3059 mL	8.6118 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (643 KB)

COA (247 KB)LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Kobusch AB, et al. Tumor-promoting activity and cytotoxicity of 3,4,3',4'-tetrachlorobiphenyl on N-nitrosomorpholine-induced murine liver foci. J Cancer Res Clin Oncol. 1989;115(3):247-52. [Content Brief]

[2]. Enzmann H, et al. Elevation of glucose-6-phosphate in early stages of hepatocarcinogenesis induced in rats by N-nitrosomorpholine. Horm Metab Res. 1988 Feb;20(2):128-9. [Content Brief]

[3]. Weber E, et al. Dose and time dependence of the cellular phenotype in rat hepatic preneoplasia and neoplasia induced by continuous oral exposure to N-nitrosomorpholine. Carcinogenesis. 1994 Jun;15(6):1235-42. [Content Brief]

[4]. K D Brunnemann, et al. N-Nitrosomorpholine and Other Volatile N-nitrosamines in Snuff Tobacco. Carcinogenesis. 1982;3(6):693-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	8.6118 mL	43.0589 mL	86.1178 mL	215.2945 mL
	5 mM	1.7224 mL	8.6118 mL	17.2236 mL	43.0589 mL
	10 mM	0.8612 mL	4.3059 mL	8.6118 mL	21.5295 mL
	15 mM	0.5741 mL	2.8706 mL	5.7412 mL	14.3530 mL
	20 mM	0.4306 mL	2.1529 mL	4.3059 mL	10.7647 mL
	25 mM	0.3445 mL	1.7224 mL	3.4447 mL	8.6118 mL
	30 mM	0.2871 mL	1.4353 mL	2.8706 mL	7.1765 mL
	40 mM	0.2153 mL	1.0765 mL	2.1529 mL	5.3824 mL
	50 mM	0.1722 mL	0.8612 mL	1.7224 mL	4.3059 mL
	60 mM	0.1435 mL	0.7176 mL	1.4353 mL	3.5882 mL
	80 mM	0.1076 mL	0.5382 mL	1.0765 mL	2.6912 mL
	100 mM	0.0861 mL	0.4306 mL	0.8612 mL	2.1529 mL