Nodosin

Name: Nodosin
Brand: MedChemExpress
SKU: HY-N3181
Rating: 4.5 (1 reviews)

Cat. No.: HY-N3181 Purity: 99.64%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Nodosin is a diterpenoid isolated from Isodon trichocarpus Kudo and I. Japonicus HARA.

For research use only. We do not sell to patients.

Nodosin Chemical Structure

CAS No. : 10391-09-0

Size	Price	Stock	Quantity
5 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 430	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Nodosin is a diterpenoid isolated from Isodon trichocarpus Kudo and I. Japonicus HARA^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 16	Cytotoxicity against human A549 cells after 48 hrs Cytotoxicity against human A549 cells after 48 hrs	[PMID: 19697926]
A549	IC₅₀	> 40 μM Compound: 9	Cytotoxicity against human A549 cells after 48 hrs by MTS assay Cytotoxicity against human A549 cells after 48 hrs by MTS assay	[PMID: 24219809]
HepG2	IC₅₀	1.92 μM Compound: 16	Cytotoxicity against human HepG2 cells after 48 hrs Cytotoxicity against human HepG2 cells after 48 hrs	[PMID: 19697926]
HL-60	IC₅₀	15 μM Compound: 9	Cytotoxicity against human HL60 cells after 48 hrs by MTS assay Cytotoxicity against human HL60 cells after 48 hrs by MTS assay	[PMID: 24219809]
K562	IC₅₀	0.54 μM Compound: 16	Cytotoxicity against human K562 cells after 48 hrs Cytotoxicity against human K562 cells after 48 hrs	[PMID: 19697926]
MCF7	IC₅₀	23.5 μM Compound: 9	Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 24219809]
RAW264.7	IC₅₀	18.4 μM Compound: 9	Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 24219809]
SMMC-7721	IC₅₀	27 μM Compound: 9	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay	[PMID: 24219809]
SW480	IC₅₀	12.1 μM Compound: 9	Cytotoxicity against human SW480 cells after 48 hrs by MTS assay Cytotoxicity against human SW480 cells after 48 hrs by MTS assay	[PMID: 24219809]
SW780	IC₅₀	9.99 μM Compound: Nodosin	Cytotoxicity against human SW780 cells assessed as reduction in cell growth by CCK8 assay Cytotoxicity against human SW780 cells assessed as reduction in cell growth by CCK8 assay	[PMID: 35976233]
T-24	IC₅₀	4.91 μM Compound: Nodosin	Cytotoxicity against human T24 cells assessed as reduction in cell growth by CCK8 assay Cytotoxicity against human T24 cells assessed as reduction in cell growth by CCK8 assay	[PMID: 35976233]
UMUC3	IC₅₀	3.45 μM Compound: Nodosin	Cytotoxicity against human UMUC3 cells assessed as reduction in cell growth by CCK8 assay Cytotoxicity against human UMUC3 cells assessed as reduction in cell growth by CCK8 assay	[PMID: 35976233]

Molecular Weight

362.42

Formula

C₂₀H₂₆O₆

CAS No.

10391-09-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@]([C@]2(C(C3=C)=O)C[C@H]3C1)([H])[C@@]([C@@](CC4)([H])OC2=O)(CO5)[C@](C4(C)C)([H])[C@@H]5O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (344.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7592 mL	13.7961 mL	27.5923 mL
5 mM	0.5518 mL	2.7592 mL	5.5185 mL
10 mM	0.2759 mL	1.3796 mL	2.7592 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (284 KB) SDS (251 KB)

COA (268 KB) HNMR (268 KB) LCMS (270 KB)

Handling Instructions (2659 KB)

References

[1]. Fujita, E, et al. Terpenoids vii the structure and absolute configuration of nodosin a new di terpenoid from isodon d species inst nuclear magnetic resonance isodon trichocarpus d isodon japonicus d.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7592 mL	13.7961 mL	27.5923 mL	68.9807 mL
	5 mM	0.5518 mL	2.7592 mL	5.5185 mL	13.7961 mL
	10 mM	0.2759 mL	1.3796 mL	2.7592 mL	6.8981 mL
	15 mM	0.1839 mL	0.9197 mL	1.8395 mL	4.5987 mL
	20 mM	0.1380 mL	0.6898 mL	1.3796 mL	3.4490 mL
	25 mM	0.1104 mL	0.5518 mL	1.1037 mL	2.7592 mL
	30 mM	0.0920 mL	0.4599 mL	0.9197 mL	2.2994 mL
	40 mM	0.0690 mL	0.3449 mL	0.6898 mL	1.7245 mL
	50 mM	0.0552 mL	0.2759 mL	0.5518 mL	1.3796 mL
	60 mM	0.0460 mL	0.2299 mL	0.4599 mL	1.1497 mL
	80 mM	0.0345 mL	0.1725 mL	0.3449 mL	0.8623 mL
	100 mM	0.0276 mL	0.1380 mL	0.2759 mL	0.6898 mL

Nodosin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nodosin10391-09-0OthersditerpenoidIsodon trichocarpusI. JaponicusInhibitorinhibitorinhibit

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Nodosin

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Complete Stock Solution Preparation Table

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