NQO1 substrate

Name: NQO1 substrate
Brand: MedChemExpress
SKU: HY-114315
Rating: 4.5 (1 reviews)

Cat. No.: HY-114315 Purity: 98.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

NQO1 substrate acts as an efficient NQO1 substrate and may be a new option for the treatment of NQO1-overexpresssing drug-resistant NSCLC.

For research use only. We do not sell to patients.

NQO1 substrate Chemical Structure

CAS No. : 2304503-05-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 121	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 121	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 305	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

NQO1 substrate acts as an efficient NQO1 substrate and may be a new option for the treatment of NQO1-overexpresssing drug-resistant NSCLC^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1 μM Compound: 20k	Cytotoxicity against human A549 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30508483]
A549/TR	IC₅₀	0.6 μM Compound: 20k	Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30508483]
A549/TR	IC₅₀	25.6 μM Compound: 20k	Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of DIC and measured after 72 hrs by MTT assay Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of DIC and measured after 72 hrs by MTT assay	[PMID: 30508483]
A549/TR	IC₅₀	33.2 μM Compound: 20k	Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of NAC and measured after 72 hrs by MTT assay Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of NAC and measured after 72 hrs by MTT assay	[PMID: 30508483]
L02	IC₅₀	30.2 μM Compound: 20k	Cytotoxicity against NQO1 deficient human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against NQO1 deficient human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30508483]
MCF7	IC₅₀	1.3 μM Compound: 20k	Cytotoxicity against human MCF7 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30508483]
MIA PaCa-2	IC₅₀	4.5 μM Compound: 20k	Cytotoxicity against human MIAPaCa2 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MIAPaCa2 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30508483]
NCI-H596	IC₅₀	19.1 μM Compound: 20k	Cytotoxicity against NQO1 deficient human NCI-H596 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against NQO1 deficient human NCI-H596 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30508483]

Molecular Weight

268.18

Formula

C₁₃H₂F₂N₄O

CAS No.

2304503-05-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

N#CC1=C(C#N)N=C2C(C(C3=C2C=C(F)C(F)=C3)=O)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (466.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7288 mL	18.6442 mL	37.2884 mL
5 mM	0.7458 mL	3.7288 mL	7.4577 mL
10 mM	0.3729 mL	1.8644 mL	3.7288 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (23.31 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.02%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (252 KB) RP-HPLC (242 KB)

Handling Instructions (2659 KB)

References

[1]. Wu X, et al. Discovery of Nonquinone Substrates for NAD(P)H: Quinone Oxidoreductase 1 (NQO1) as Effective Intracellular ROS Generators for the Treatment of Drug-Resistant Non-Small-Cell Lung Cancer. J Med Chem. 2018 Dec 27;61(24):11280-11297. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7288 mL	18.6442 mL	37.2884 mL	93.2210 mL
	5 mM	0.7458 mL	3.7288 mL	7.4577 mL	18.6442 mL
	10 mM	0.3729 mL	1.8644 mL	3.7288 mL	9.3221 mL
	15 mM	0.2486 mL	1.2429 mL	2.4859 mL	6.2147 mL
	20 mM	0.1864 mL	0.9322 mL	1.8644 mL	4.6610 mL
	25 mM	0.1492 mL	0.7458 mL	1.4915 mL	3.7288 mL
	30 mM	0.1243 mL	0.6215 mL	1.2429 mL	3.1074 mL
	40 mM	0.0932 mL	0.4661 mL	0.9322 mL	2.3305 mL
	50 mM	0.0746 mL	0.3729 mL	0.7458 mL	1.8644 mL
	60 mM	0.0621 mL	0.3107 mL	0.6215 mL	1.5537 mL
	80 mM	0.0466 mL	0.2331 mL	0.4661 mL	1.1653 mL
	100 mM	0.0373 mL	0.1864 mL	0.3729 mL	0.9322 mL

NQO1 substrate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NQO1 substrate2304503-05-5OthersInhibitorinhibitorinhibit

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NQO1 substrate

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NQO1 substrate Related Antibodies

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Complete Stock Solution Preparation Table

NQO1 substrate Related Classifications

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