2. Metabolic Enzyme/Protease
  3. MMP
  4. o-Phenanthroline monohydrate

o-Phenanthroline monohydrate  (Synonyms: 1,10-Phenanthroline monohydrate)

Cat. No.: HY-Y1841 Purity: 99.38%
SDS COA Handling Instructions

Molarity Calculator

o-Phenanthroline (1,10-Phenanthroline) monohydrate, a metal chelator, prevents the induction of chromosomal aberrations in streptozotocin-treated cells. o-Phenanthroline monohydrate forms a red chelate with Fe2+ that absorbs maximally at 510 nm. o-Phenanthroline (1,10-Phenanthroline) monohydrate is also a MMP inhibitor.

For research use only. We do not sell to patients.

o-Phenanthroline monohydrate Chemical Structure

o-Phenanthroline monohydrate Chemical Structure

CAS No. : 5144-89-8

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of o-Phenanthroline monohydrate:

o-Phenanthroline monohydrate Related Antibodies

Top Publications Citing Use of Products

View All MMP Isoform Specific Products:

View All Isoforms
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

o-Phenanthroline (1,10-Phenanthroline) monohydrate, a metal chelator, prevents the induction of chromosomal aberrations in streptozotocin-treated cells. o-Phenanthroline monohydrate forms a red chelate with Fe2+ that absorbs maximally at 510 nm. o-Phenanthroline (1,10-Phenanthroline) monohydrate is also a MMP inhibitor[1][2].

In Vitro

o-Phenanthroline (1,10-Phenanthroline, 1mM) inhibits autolysis of sea cucumber body wall[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

o-Phenanthroline monohydrate Related Antibodies
Molecular Weight

198.22

Formula

C12H10N2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C12=C(N=CC=C3)C3=CC=C1C=CC=N2.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (504.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 2 mg/mL (10.09 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.0449 mL 25.2245 mL 50.4490 mL
5 mM 1.0090 mL 5.0449 mL 10.0898 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.38%

SDS (623 KB)

COA (254 KB) HNMR (96 KB) RP-HPLC (150 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.0449 mL 25.2245 mL 50.4490 mL 126.1225 mL
5 mM 1.0090 mL 5.0449 mL 10.0898 mL 25.2245 mL
10 mM 0.5045 mL 2.5224 mL 5.0449 mL 12.6122 mL
DMSO 15 mM 0.3363 mL 1.6816 mL 3.3633 mL 8.4082 mL
20 mM 0.2522 mL 1.2612 mL 2.5224 mL 6.3061 mL
25 mM 0.2018 mL 1.0090 mL 2.0180 mL 5.0449 mL
30 mM 0.1682 mL 0.8408 mL 1.6816 mL 4.2041 mL
40 mM 0.1261 mL 0.6306 mL 1.2612 mL 3.1531 mL
50 mM 0.1009 mL 0.5045 mL 1.0090 mL 2.5224 mL
60 mM 0.0841 mL 0.4204 mL 0.8408 mL 2.1020 mL
80 mM 0.0631 mL 0.3153 mL 0.6306 mL 1.5765 mL
100 mM 0.0504 mL 0.2522 mL 0.5045 mL 1.2612 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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o-Phenanthroline monohydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

o-Phenanthroline5144-89-81,10-PhenanthrolineMMPMatrix metalloproteinasesInhibitorinhibitorinhibit

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