2. Autophagy
  3. p62 Mitophagy Autophagy
  4. P62-mediated mitophagy inducer

P62-mediated mitophagy inducer  (Synonyms: PMI)

Cat. No.: HY-115576 Purity: 99.49%
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P62-mediated mitophagy inducer (PMI) is a P62-mediated mitophagy activator. P62-mediated mitophagy inducer activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential and remains active in cells lacking a fully functional PINK1/Parkin pathway. P62-mediated mitophagy inducer serves as a pharmacological tool to study the molecular mechanisms of mitosis, avoiding toxicity and some of the non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential.

For research use only. We do not sell to patients.

P62-mediated mitophagy inducer Chemical Structure

P62-mediated mitophagy inducer Chemical Structure

CAS No. : 1809031-84-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 In-stock
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Solid
5 mg USD 80 In-stock
10 mg USD 130 In-stock
25 mg USD 260 In-stock
50 mg USD 420 In-stock
100 mg USD 670 In-stock
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Based on 4 publication(s) in Google Scholar

P62-mediated mitophagy inducer Related Antibodies

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Description

P62-mediated mitophagy inducer (PMI) is a P62-mediated mitophagy activator. P62-mediated mitophagy inducer activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential and remains active in cells lacking a fully functional PINK1/Parkin pathway. P62-mediated mitophagy inducer serves as a pharmacological tool to study the molecular mechanisms of mitosis, avoiding toxicity and some of the non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential[1].

IC50 & Target

mitophagy[1].
In Vitro

P62-mediated mitophagy inducer (10 μM; 0, 1, 3, 6, 24 h) stabilizes Nrf2 and (10 μM; 9 h) upregulates P62 expression activating mitophagy[1].
? P62-mediated mitophagy (10 μM; 24 h) acts downstream of the PINK1/Parkin signaling pathway in MEFs[1].
? P62-mediated mitophagy inducer positively affects mitochondrial poly-ubiquitination and coupling[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

P62-mediated mitophagy inducer Related Antibodies

RT-PCR[1]

Cell Line: MEFs
Concentration: 10 µM
Incubation Time: 9 h
Result: Significantly increased p62 mRNA levels.

Immunofluorescence[1]

Cell Line: MEFs
Concentration: 10 µM
Incubation Time: 24 h
Result: Demonstrated Parkin-independent induction of mitochondrial recruitment of P62.

Western Blot Analysis[1]

Cell Line: MEFs
Concentration: 10 µM
Incubation Time: 0, 1, 3, 6, 24 h
Result: Exhibited maximum Nrf2 levels after 6 h and remained elevated at 24 h.
Clinical Trial
Molecular Weight

392.15

Formula

C14H9IN4O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+](C1=CC(C2=CN(C3=CC=CC(I)=C3)N=N2)=CC=C1)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (21.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5500 mL 12.7502 mL 25.5004 mL
5 mM 0.5100 mL 2.5500 mL 5.1001 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (3.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.25 mg/mL (3.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.49%

SDS (252 KB)

COA (248 KB) RP-HPLC (241 KB) Elemental Analysis Report (107 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5500 mL 12.7502 mL 25.5004 mL 63.7511 mL
5 mM 0.5100 mL 2.5500 mL 5.1001 mL 12.7502 mL
10 mM 0.2550 mL 1.2750 mL 2.5500 mL 6.3751 mL
15 mM 0.1700 mL 0.8500 mL 1.7000 mL 4.2501 mL
20 mM 0.1275 mL 0.6375 mL 1.2750 mL 3.1876 mL
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P62-mediated mitophagy inducer Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

P62-mediated mitophagy inducer1809031-84-2PMIp62MitophagyAutophagySequestosome-1SQSTM1Mitochondrial AutophagymitosisautophagyMEFsPINK1/ParkinNrf2cancerneurodegenerative diseasesInhibitorinhibitorinhibit

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