PDE4B-IN-3

Name: PDE4B-IN-3
Brand: MedChemExpress
SKU: HY-147830
Rating: 4.5 (1 reviews)

Cat. No.: HY-147830 Purity: 99.40%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PDE4B-IN-3 is a potent PDE4B inhibitor with an IC₅₀ of 0.94 μM. PDE4B-IN-3 has anti-inflammatory activities.

For research use only. We do not sell to patients.

PDE4B-IN-3 Chemical Structure

CAS No. : 2819779-01-4

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1800	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
Customer Review

Description

PDE4B-IN-3 is a potent PDE4B inhibitor with an IC₅₀ of 0.94 μM. PDE4B-IN-3 has anti-inflammatory activities^[1].

IC₅₀ & Target^[1]

PDE4B

.94 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	18.98 μM Compound: f4	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-1beta production measured after 24 hrs by ELISA Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-1beta production measured after 24 hrs by ELISA	[PMID: 35660249]
RAW264.7	IC₅₀	20.4 μM Compound: f4	Inhibition of LPS induced NO production in mouse RAW264.7 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by Griess reagent based assay Inhibition of LPS induced NO production in mouse RAW264.7 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by Griess reagent based assay	[PMID: 35660249]
RAW264.7	IC₅₀	23.48 μM Compound: f4	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production measured after 24 hrs by ELISA Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production measured after 24 hrs by ELISA	[PMID: 35660249]

In Vitro

PDE4B-IN-3 (compound f4) has good inhibitory activity on the production of NO, TNF-α and IL-1β with IC₅₀ values of 20.40 μM, 23.48 μM, and 18.98 μM in RAW264.7 cells. PDE4B-IN-3 could also inhibit the expression of iNOS and COX-2 in RAW264.7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In vivo studies shows that PDE4B-IN-3 (compound f4; 10-30 mg/kg) could improve the degree of foot swelling and knee joint pathology in adjuvant-induced arthritic rats and decrease the levels of serum inflammatory factors TNF-α and IL-1β in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

565.75

Formula

C₃₀H₃₅N₃O₄S₂

CAS No.

2819779-01-4

Appearance

Solid

Color

White to off-white

SMILES

CC(C1=CC(C(C)C)=C(C(C(C)C)=C1)S(=O)(NC2=CC(NS(=O)(C3=CC=CC4=CC=CN=C43)=O)=CC=C2)=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (88.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7676 mL	8.8378 mL	17.6757 mL
5 mM	0.3535 mL	1.7676 mL	3.5351 mL
10 mM	0.1768 mL	0.8838 mL	1.7676 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (4.42 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (252 KB) HNMR (120 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. Siqi Xing, et al. Novel quinoline-based derivatives: A new class of PDE4B inhibitors for adjuvant-induced arthritis. Eur J Med Chem. 2022 Aug 5;238:114497. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7676 mL	8.8378 mL	17.6757 mL	44.1891 mL
	5 mM	0.3535 mL	1.7676 mL	3.5351 mL	8.8378 mL
	10 mM	0.1768 mL	0.8838 mL	1.7676 mL	4.4189 mL
	15 mM	0.1178 mL	0.5892 mL	1.1784 mL	2.9459 mL
	20 mM	0.0884 mL	0.4419 mL	0.8838 mL	2.2095 mL
	25 mM	0.0707 mL	0.3535 mL	0.7070 mL	1.7676 mL
	30 mM	0.0589 mL	0.2946 mL	0.5892 mL	1.4730 mL
	40 mM	0.0442 mL	0.2209 mL	0.4419 mL	1.1047 mL
	50 mM	0.0354 mL	0.1768 mL	0.3535 mL	0.8838 mL
	60 mM	0.0295 mL	0.1473 mL	0.2946 mL	0.7365 mL
	80 mM	0.0221 mL	0.1105 mL	0.2209 mL	0.5524 mL

PDE4B-IN-3 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PDE4B-IN-32819779-01-4Phosphodiesterase (PDE)anti-inflammatory activityNOTNF-αIL-1βiNOSCOX-2Inhibitorinhibitorinhibit

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PDE4B-IN-3

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PDE4B-IN-3 Related Antibodies

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•Related Small Molecules:

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View All Phosphodiesterase (PDE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

PDE4B-IN-3 Related Antibodies

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Complete Stock Solution Preparation Table

PDE4B-IN-3 Related Classifications

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