1. GPCR/G Protein Neuronal Signaling
  2. Dopamine Receptor
  3. Pramipexole dihydrochloride hydrate

Pramipexole dihydrochloride hydrate 

Cat. No.: HY-B0410A Purity: ≥98.0%
COA Handling Instructions

Pramipexole dihydrochloride hydrate is a selective and blood-brain barrier (BBB) penetrant dopamine D2-type receptor agonist, with Kis of 2.2 nM, 3.9 nM, 0.5 nM and 1.3 nM for D2-type receptor, D2, D3 and D4 receptors, respectively. Pramipexole dihydrochloride hydrate can be used for the research of Parkinson's disease (PD) and restless legs syndrome (RLS).

For research use only. We do not sell to patients.

Pramipexole dihydrochloride hydrate Chemical Structure

Pramipexole dihydrochloride hydrate Chemical Structure

CAS No. : 191217-81-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
5 mg USD 32 In-stock
10 mg USD 50 In-stock
50 mg USD 150 In-stock
100 mg   Get quote  
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Pramipexole dihydrochloride hydrate:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pramipexole dihydrochloride hydrate is a selective and blood-brain barrier (BBB) penetrant dopamine D2-type receptor agonist, with Kis of 2.2 nM, 3.9 nM, 0.5 nM and 1.3 nM for D2-type receptor, D2, D3 and D4 receptors, respectively. Pramipexole dihydrochloride hydrate can be used for the research of Parkinson's disease (PD) and restless legs syndrome (RLS)[1][2][3].

IC50 & Target[1]

D2 Receptor

3.9 nM (Ki)

D3 Receptor

0.5 nM (Ki)

D4 Receptor

1.3 nM (Ki)

In Vitro

Pramipexole dihydrochloride hydrate shows a low binding affinity for D1-type receptor, with an IC50 of >50,000 nM[1].
Pramipexole dihydrochloride hydrate (0.01-10 μM; 72 hours) produces dose-dependent increases of dendritic arborization and soma size[3].
Pramipexole dihydrochloride hydrate attenuates levodopa-induced toxicity in mesencephalic cultures[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pramipexole dihydrochloride hydrate (0.25-1 mg/kg; i.p.) significantly reduces the infarction volume in animals[5].
Pramipexole dihydrochloride hydrate improves neurological recovery[5].
Pramipexole dihydrochloride hydrate prevents ischemic cell death via mitochondrial pathways in ischemic stroke[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats weighing 250-300 g (16-18 weeks old)[5]
Dosage: 0.25 mg/kg, 1 mg/kg
Administration: Intraperitoneal injection
Result: Decreased infarction volume as compared to tMCAO (transient middle cerebral artery occlusion)-only animals.
Clinical Trial
Molecular Weight

302.26

Formula

C10H21Cl2N3OS

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[H]Cl.NC1=NC(CC[C@H](NCCC)C2)=C2S1.[H]Cl.O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (330.84 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3084 mL 16.5420 mL 33.0841 mL
5 mM 0.6617 mL 3.3084 mL 6.6168 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2 mg/mL (6.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (6.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (330.84 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.3084 mL 16.5420 mL 33.0841 mL 82.7102 mL
5 mM 0.6617 mL 3.3084 mL 6.6168 mL 16.5420 mL
10 mM 0.3308 mL 1.6542 mL 3.3084 mL 8.2710 mL
15 mM 0.2206 mL 1.1028 mL 2.2056 mL 5.5140 mL
20 mM 0.1654 mL 0.8271 mL 1.6542 mL 4.1355 mL
25 mM 0.1323 mL 0.6617 mL 1.3234 mL 3.3084 mL
30 mM 0.1103 mL 0.5514 mL 1.1028 mL 2.7570 mL
40 mM 0.0827 mL 0.4136 mL 0.8271 mL 2.0678 mL
50 mM 0.0662 mL 0.3308 mL 0.6617 mL 1.6542 mL
60 mM 0.0551 mL 0.2757 mL 0.5514 mL 1.3785 mL
80 mM 0.0414 mL 0.2068 mL 0.4136 mL 1.0339 mL
100 mM 0.0331 mL 0.1654 mL 0.3308 mL 0.8271 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pramipexole dihydrochloride hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pramipexole dihydrochloride hydrate
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