2. GPCR/G Protein Neuronal Signaling
  3. Dopamine Receptor
  4. Pramipexole

Pramipexole is a selective and blood-brain barrier (BBB) penetrant dopamine D2-type receptor agonist, with Kis of 2.2 nM, 3.9 nM, 0.5 nM and 1.3 nM for D2-type receptor, D2, D3 and D4 receptors, respectively. Pramipexole can be used for the research of Parkinson's disease (PD) and restless legs syndrome (RLS).

For research use only. We do not sell to patients.

Pramipexole Chemical Structure

Pramipexole Chemical Structure

CAS No. : 104632-26-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 39 In-stock
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Based on 5 publication(s) in Google Scholar

Other Forms of Pramipexole:

Pramipexole Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pramipexole is a selective and blood-brain barrier (BBB) penetrant dopamine D2-type receptor agonist, with Kis of 2.2 nM, 3.9 nM, 0.5 nM and 1.3 nM for D2-type receptor, D2, D3 and D4 receptors, respectively. Pramipexole can be used for the research of Parkinson's disease (PD) and restless legs syndrome (RLS)[1][2][3].

IC50 & Target[1]

D2 Receptor

3.9 nM (Ki)

D3 Receptor

0.5 nM (Ki)

D4 Receptor

1.3 nM (Ki)

In Vitro

Pramipexole shows a low binding affinity for D1-type receptor, with an IC50 of >50,000 nM[1].
Pramipexole (0.01-10 μM; 72 hours) produces dose-dependent increases of dendritic arborization and soma size[3].
Pramipexole attenuates levodopa-induced toxicity in mesencephalic cultures[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pramipexole Related Antibodies
In Vivo

Pramipexole (0.25-1 mg/kg; i.p.) significantly reduces the infarction volume in animals[5].
Pramipexole improves neurological recovery[5].
Pramipexole prevents ischemic cell death via mitochondrial pathways in ischemic stroke[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats weighing 250-300 g (16-18 weeks old)[5]
Dosage: 0.25 mg/kg, 1 mg/kg
Administration: Intraperitoneal injection
Result: Decreased infarction volume as compared to tMCAO (transient middle cerebral artery occlusion)-only animals.
Clinical Trial
Molecular Weight

211.33

Formula

C10H17N3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC(CC[C@H](NCCC)C2)=C2S1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (473.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7319 mL 23.6597 mL 47.3194 mL
5 mM 0.9464 mL 4.7319 mL 9.4639 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 10 mg/mL (47.32 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 10 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.95%

COA (242 KB) HNMR (127 KB) RP-HPLC (192 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.7319 mL 23.6597 mL 47.3194 mL 118.2984 mL
5 mM 0.9464 mL 4.7319 mL 9.4639 mL 23.6597 mL
10 mM 0.4732 mL 2.3660 mL 4.7319 mL 11.8298 mL
15 mM 0.3155 mL 1.5773 mL 3.1546 mL 7.8866 mL
20 mM 0.2366 mL 1.1830 mL 2.3660 mL 5.9149 mL
25 mM 0.1893 mL 0.9464 mL 1.8928 mL 4.7319 mL
30 mM 0.1577 mL 0.7887 mL 1.5773 mL 3.9433 mL
40 mM 0.1183 mL 0.5915 mL 1.1830 mL 2.9575 mL
50 mM 0.0946 mL 0.4732 mL 0.9464 mL 2.3660 mL
60 mM 0.0789 mL 0.3943 mL 0.7887 mL 1.9716 mL
80 mM 0.0591 mL 0.2957 mL 0.5915 mL 1.4787 mL
100 mM 0.0473 mL 0.2366 mL 0.4732 mL 1.1830 mL
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Pramipexole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pramipexole104632-26-0Dopamine ReceptorParkinson'sdiseasePDrestlesslegssyndromeRLSneurologicalrecoveryneuroprotectiontMCAOtransientmiddlecerebralarteryocclusionInhibitorinhibitorinhibit

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