2. Apoptosis
  3. Apoptosis
  4. Procyanidin C1

Procyanidin C1  (Synonyms: PCC1)

Cat. No.: HY-N2342 Purity: 99.79%
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Procyanidin C1 (PCC1), a natural polyphenol with oral activity, causes DNA damage, cell cycle arrest and induces apoptosis. Procyanidin C1 decreases the level of Bcl-2, but enhances BAX, caspase 3 and 9 expression in cancer cells. Procyanidin C1 shows senotherapeutic activity and increases lifespan in mice.

For research use only. We do not sell to patients.

Procyanidin C1 Chemical Structure

Procyanidin C1 Chemical Structure

CAS No. : 37064-30-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 620 In-stock
Solution
10 mM * 1 mL in DMSO USD 620 In-stock
Solid
1 mg USD 90 In-stock
5 mg USD 390 In-stock
10 mg USD 650 In-stock
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100 mg   Get quote  

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Based on 3 publication(s) in Google Scholar

Other Forms of Procyanidin C1:

Procyanidin C1 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Procyanidin C1 (PCC1), a natural polyphenol with oral activity, causes DNA damage, cell cycle arrest and induces apoptosis. Procyanidin C1 decreases the level of Bcl-2, but enhances BAX, caspase 3 and 9 expression in cancer cells. Procyanidin C1 shows senotherapeutic activity and increases lifespan in mice[1][2].

IC50 & Target

IC50: 31.5 μg/mL (MCF-7), 36.6 μg/mL (MDA-MB-231)[1]
Cellular Effect
Cell Line Type Value Description References
A549 ED50
> 10 μg/mL
Compound: 49
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
HCT-8 ED50
> 10 μg/mL
Compound: 49
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
KB ED50
> 10 μg/mL
Compound: 49
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
MT4 CC50
16.5 μg/mL
Compound: 10, proanthocyanidin C1
Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
[PMID: 10425115]
MT4 IC50
4.3 μg/mL
Compound: 10, proanthocyanidin C1
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
[PMID: 10425115]
TE-671 ED50
> 10 μg/mL
Compound: 49
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
In Vitro

Procyanidin C1 (6.25-100 μg/mL; 48 h) shows cytotoxic activities to MCF-7 and MDA-MB-231 cells[1].
Procyanidin C1 (35 μg/mL; 48 h) affects cell cycle of MCF-7 and MDA-MB-231 cancer cells[1].
Procyanidin C1 significantly up-regulates Chk 1 and Chk 2 in MCF-7 and MDA-MB-231 cancer cells[1].
Procyanidin C1 (27.85 and 66.41 μL) induces significant DNA damage in MCF-7 and MDA-MB-231 cancer cells[1].
Procyanidin C1 (45 μg/mL; 72 h) reduces the expression level of Bcl-2 but increases the expression level of BAX, and increases activities of caspase 3 and 9 to induces cell apoptosis of MCF-7 and MDA-MB-231 cancer cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Procyanidin C1 Related Antibodies

Cell Cycle Analysis[1]

Cell Line: MCF-7 and MDA-MB-231 cell lines
Concentration: 35 μg/mL
Incubation Time: 48 h
Result: Induced the cell cycle arrest at the S-phase in MCF-7 and MDA-MB-231 cell lines.

Cell Cytotoxicity Assay[1]

Cell Line: MCF-7 and MDA-MB-231 cell lines
Concentration: 6.25-100 μg/mL
Incubation Time: 48 h
Result: Inhibited cell proliferation of MCF-7 and MDA-MB-231 cells with IC50 values of 31.5 and 36.6 μg/mL and showed a higher cytotoxic activity to MDA-MB-231 than tamoxifen.
In Vivo

Procyanidin C1 (20 mg/kg; i.p.; 2 weeks after the first MIT dose and then delivered biweekly) increases tumour regression[2].
Procyanidin C1 (20 mg/kg; i.p.; for 7 d) shows senolytic efficacy in mice with senescent mouse embryonic fibroblasts injection[2].
Procyanidin C1 (20 mg/kg; p.o.; for 3 d) increases the lifespan of old mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Non-obese diabetes and severe combined immunodficiency mice with PSC27 and PC3 cancer cells injection, and pre-treated with mitoxantrone (MIT)[2]
Dosage: 20 mg/kg
Administration: Intraperitoneal injection; 20 mg/kg; 2 weeks after the first MIT dose and then delivered biweekly
Result: Remarkably enhanced tumour regression (55.2% reduction in tumour size compared with MIT alone; 74.9% reduction in tumour volume compared with the placebo treatment) and depleted the majority of senescent cells in chemotherapy treated animals.
Animal Model: 24-27 months of age mice (both sexes)[2]
Dosage: 20 mg/kg
Administration: Oral gavage; 20 mg/kg; for three consecutive days
Result: Enhanced the median post-treatment lifespan with 64.2% and decreased the mortality hazard than the vehicle-treated group.
Molecular Weight

866.77

Formula

C45H38O18
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=C2C(O[C@H](C3=CC(O)=C(O)C=C3)[C@H](O)[C@H]2C4=C5C(C[C@@H](O)[C@@H](C6=CC(O)=C(O)C=C6)O5)=C(O)C=C4O)=C([C@H]7C8=C(O)C=C(O)C=C8O[C@H](C9=CC(O)=C(O)C=C9)[C@@H]7O)C(O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (115.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (57.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1537 mL 5.7685 mL 11.5371 mL
5 mM 0.2307 mL 1.1537 mL 2.3074 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 6.25 mg/mL (7.21 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.79%

SDS (393 KB)

COA (264 KB) HNMR (348 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.1537 mL 5.7685 mL 11.5371 mL 28.8427 mL
5 mM 0.2307 mL 1.1537 mL 2.3074 mL 5.7685 mL
10 mM 0.1154 mL 0.5769 mL 1.1537 mL 2.8843 mL
15 mM 0.0769 mL 0.3846 mL 0.7691 mL 1.9228 mL
20 mM 0.0577 mL 0.2884 mL 0.5769 mL 1.4421 mL
25 mM 0.0461 mL 0.2307 mL 0.4615 mL 1.1537 mL
30 mM 0.0385 mL 0.1923 mL 0.3846 mL 0.9614 mL
40 mM 0.0288 mL 0.1442 mL 0.2884 mL 0.7211 mL
50 mM 0.0231 mL 0.1154 mL 0.2307 mL 0.5769 mL
DMSO 60 mM 0.0192 mL 0.0961 mL 0.1923 mL 0.4807 mL
80 mM 0.0144 mL 0.0721 mL 0.1442 mL 0.3605 mL
100 mM 0.0115 mL 0.0577 mL 0.1154 mL 0.2884 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Procyanidin C1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Procyanidin C137064-30-5PCC1Procyanidin C 1Procyanidin C-1PCC 1PCC-1ApoptosistamoxifenDNA damagecaspaseBAXBcl-2senotherapeuticSASPpolyphenolmitoxantroneMITInhibitorinhibitorinhibit

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