2. Epigenetics Immunology/Inflammation Vitamin D Related/Nuclear Receptor
  3. Histone Acetyltransferase Toll-like Receptor (TLR) Androgen Receptor
  4. Procyanidin B3

Procyanidin B3 is a natural product with antioxidant activity and oral bioavailability, possessing good blood-brain barrier penetration. Procyanidin B3 is a selective inhibitor of histone acetyltransferase (HAT). By inhibiting p300 HAT-mediated acetylation of the androgen receptor (androgen receptor). Procyanidin B3 alleviates intervertebral disc degeneration (IVDD) by inhibiting the formation of the TLR4/MD-2 complex. Procyanidin B3 can be used in research on prostate cancer and arthritis.

For research use only. We do not sell to patients.

Procyanidin B3 Chemical Structure

Procyanidin B3 Chemical Structure

CAS No. : 23567-23-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 216 In-stock
Solution
10 mM * 1 mL in DMSO USD 216 In-stock
Solid
1 mg USD 55 In-stock
5 mg USD 170 In-stock
10 mg USD 270 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Procyanidin B3:

Procyanidin B3 Related Antibodies

Top Publications Citing Use of Products

View All Histone Acetyltransferase Isoform Specific Products:

View All Isoforms
CBP/p300 GCN5/PCAF TIP60 MOZ/MORF HBO1

View All Toll-like Receptor (TLR) Isoform Specific Products:

View All Isoforms
TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Procyanidin B3 is a natural product with antioxidant activity and oral bioavailability, possessing good blood-brain barrier penetration. Procyanidin B3 is a selective inhibitor of histone acetyltransferase (HAT). By inhibiting p300 HAT-mediated acetylation of the androgen receptor (androgen receptor). Procyanidin B3 alleviates intervertebral disc degeneration (IVDD) by inhibiting the formation of the TLR4/MD-2 complex. Procyanidin B3 can be used in research on prostate cancer and arthritis[1][2][3][4][5].

IC50 & Target

CBP/p300

 

TLR4

 

Cellular Effect
Cell Line Type Value Description References
MT4 CC50
117.8 μg/mL
Compound: 3, procyanidin B3
Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
[PMID: 10425115]
MT4 IC50
27 μg/mL
Compound: 3, procyanidin B3
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
[PMID: 10425115]
In Vitro

Procyanidin B3 (50 and 100 μM, 1 h) inhibits the AR(androgen receptor)-dependent promoter activity induced by the synthetic androgen R1881 in LNCaP cells[1].
Procyanidin B3 (25-100 μM, 1 h) inhibits p300 HAT activity and p300-mediated AR (androgen receptor) acetylation in a dose-dependent manner in LNCaP cells[1].
Procyanidin B3 (50 μM, 12 hours) enhances the antagonistic effect of Flutamide (HY-B0022) by inhibiting p300 activity[1].
Procyanidin B3 (40 μM, 24 and 48 h) significantly upregulates the cell viability of human nucleus pulposus (NP) cells[4].
Procyanidin B3 (2.5-40 μM, 24 h) inhibits the inflammatory effects in human nucleus pulposus (NP) cells exposed to lipopolysaccharide (HY-D1056)[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Procyanidin B3 Related Antibodies

Cell Viability Assay[4]

Cell Line: Human nucleus pulposus (NP) cells
Concentration: 0, 1, 2.5, 10, 40, 160 μM
Incubation Time: 24 h and 48 h
Result: Significantly up-regulated cell viability with 40 µmol/L for 24 and 48 hours.

RT-PCR[1]

Cell Line: Human LNCap
Concentration: 50 and 100 μM
Incubation Time: 1 h
Result: Inhibited the synthetic androgen R1881-induced AR-dependent promoter activity.
Resulted in the transcription levels of NKX3.1 and PSA genes being reduced to levels similar to those with Flutamide (HY-B0022) treatment. Combined treatment with Flutamide (HY-B0022) increased the inhibition of p300-mediated AR transcription.

Western Blot Analysis[4]

Cell Line: Human nucleus pulposus (NP) cells
Concentration: 2.5, 10, 40 μM
Incubation Time: 24 h
Result: Suppressed the expression levels of inflammation-related regulatory factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase.
Reversed the Lipopolysaccharides (HY-D1056)-induced changes in NF-κB signaling pathway protein expression at the dose of 40 μM, including increased cytoplasmic IκBα expression and decreased nuclear p65 expression.
In Vivo

Procyanidin B3 (100 mg/kg, p.o., 5 days a week for 4 weeks) prevents articular cartilage degeneration and heterotopic cartilage formation in a surgically-induced osteoarthritis mice model[2].
Procyanidin B3 (30 mg/kg, p.o., once daily for 4 weeks) delays the development of intervertebral disc degeneration (IVDD) in a Lipopolysaccharides (HY-D1056)-induced IVDD Sprague-Dawley rats model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Lipopolysaccharides (HY-D1056)-induced IVDD (intervertebral disc degeneration) Sprague-Dawley rats model
Dosage: 30 mg/kg
Administration: Oral gavage (p.o.),once daily for 4 weeks
Result: Prevented the loss of gelatin NP cells and structural damage of annulus fibrosus in IVDD rat model.
Animal Model: Surgically-induced osteoarthritis mice model[2]
Dosage: 100 mg/kg
Administration: Oral gavage (p.o.), once daily, 5 days a week for 4 weeks
Result: Prevented aberrant articular cartilage degeneration and heterotopic cartilage formation through suppression of the nitric oxide synthase (iNOS).
Molecular Weight

578.52

Formula

C30H26O12
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O[C@@H]1[C@@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3[C@H]1C4=C5C(C[C@H](O)[C@@H](C6=CC=C(O)C(O)=C6)O5)=C(O)C=C4O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (86.43 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7285 mL 8.6427 mL 17.2855 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.63%

SDS (393 KB)

COA (259 KB) HNMR (313 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7285 mL 8.6427 mL 17.2855 mL 43.2137 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL 8.6427 mL
10 mM 0.1729 mL 0.8643 mL 1.7285 mL 4.3214 mL
15 mM 0.1152 mL 0.5762 mL 1.1524 mL 2.8809 mL
20 mM 0.0864 mL 0.4321 mL 0.8643 mL 2.1607 mL
25 mM 0.0691 mL 0.3457 mL 0.6914 mL 1.7285 mL
30 mM 0.0576 mL 0.2881 mL 0.5762 mL 1.4405 mL
40 mM 0.0432 mL 0.2161 mL 0.4321 mL 1.0803 mL
50 mM 0.0346 mL 0.1729 mL 0.3457 mL 0.8643 mL
60 mM 0.0288 mL 0.1440 mL 0.2881 mL 0.7202 mL
80 mM 0.0216 mL 0.1080 mL 0.2161 mL 0.5402 mL
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Procyanidin B3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Procyanidin B323567-23-9Procyanidin B 3Procyanidin B-3Histone AcetyltransferaseToll-like Receptor (TLR)Androgen ReceptorHATsHATSurgically-induced osteoarthritis mice modelHuman LNCapAnti-inflammatory activitiesAnticancer activitiesAntioxidant activityInhibitorinhibitorinhibit

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