Prostratin

Name: Prostratin
Brand: MedChemExpress
SKU: HY-107421
Rating: 4.5 (1 reviews)

Cat. No.: HY-107421 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

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Prostratin, a natural terpenoid compound, is a PKC activator, with a K_i of 12.5 nM and shows inhibitory effect on HIV-1.

For research use only. We do not sell to patients.

Prostratin Chemical Structure

CAS No. : 60857-08-1

Size	Price	Stock	Quantity
1 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

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HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Prostratin, a natural terpenoid compound, is a PKC activator, with a K_i of 12.5 nM and shows inhibitory effect on HIV-1.

IC₅₀ & TargetPKC PKC ^[1]

PKC

12.5 nM (Ki)

HIV-1

Cellular Effect

Cell Line	Type	Value	Description	References
C8166	CC₅₀	> 0.51 μM Compound: 4, Prostratin	Cytotoxicity against human C8166 cells by MTT assay Cytotoxicity against human C8166 cells by MTT assay	[PMID: 21534540]
KB	IC₅₀	1000 μg/mL Compound: 11	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 17512094]
MT2	CC₅₀	> 50 μM Compound: Prostratin	Cytotoxicity against human MT2 cells by Celltiter-Glo assay Cytotoxicity against human MT2 cells by Celltiter-Glo assay	[PMID: 28925702]
MT4	EC₅₀	0.9 μM Compound: 23, prostratin	Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay Antiviral activity against HIV2 ROD infected in MT4 cells assessed as cell viability after 5 days by MTT assay	[PMID: 25970561]
MT4	EC₅₀	1.9 μM Compound: 23, prostratin	Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay Antiviral activity against HIV1 3B infected in MT4 cells assessed as cell viability after 5 days by MTT assay	[PMID: 25970561]
Ramos	IC₅₀	0.056 μg/mL Compound: 9	Cytotoxicity against human Ramos B cells after 72 hrs by MTT assay Cytotoxicity against human Ramos B cells after 72 hrs by MTT assay	[PMID: 16792421]
Vero	EC₅₀	> 256 μM Compound: 2	Antiviral activity against Semliki forest virus infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay Antiviral activity against Semliki forest virus infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay	[PMID: 23215460]
Vero	EC₅₀	> 256 μM Compound: 2	Antiviral activity against Sindbis virus HRsp infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay Antiviral activity against Sindbis virus HRsp infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay	[PMID: 23215460]
Vero	EC₅₀	2.6 μM Compound: 2	Antiviral activity against Chikungunya virus 899 infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay Antiviral activity against Chikungunya virus 899 infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect incubated for 6 to 7 days by MTS assay	[PMID: 23215460]
Vero	EC₅₀	2.7 μM Compound: 23, prostratin	Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days Antiviral activity against chikungunya virus Indian ocean strain 899 infected in Vero cells assessed as virus-induced cytopathic effect after 6 to 7 days	[PMID: 25970561]
Vero	CC₅₀	79 μM Compound: 2	Cytotoxicity against african green monkey Vero cells by MTS assay Cytotoxicity against african green monkey Vero cells by MTS assay	[PMID: 23215460]
Vero	CC₅₀	79 μM Compound: Prostratin	Cytotoxicity against African green monkey Vero cells assessed as morphological changes by microscopic method Cytotoxicity against African green monkey Vero cells assessed as morphological changes by microscopic method	[PMID: 28925702]

In Vitro

Prostratin inhibits [³H]PDBu binding to the CEM cells with a K_i of 210 nM^[1].
Prostratin (125-1000 nM) dose-dependently inhibits the growth of acute myeloid leukemia (AML) cell lines (HL-60, NB4, and U937 cells). Prostratin (125-100 nM) induces G1 arrest of AML cells and affects the cell-cycle-related molecules (pRb phosphorylation, CDKs, and p21) in HL-60 cells. Prostratin also causes differentiation in AML cell lines via activation of PKC. Furthermore, PKC-dependent activation of the MEK/ERK/MAP signaling pathway requires differentiation induced by Prostratin^[2].
Prostratin induces HIV-1 transcription activation requiring active form of PKD3. Prostratin also activates PKD3 via PKCε of novel PKC subfamily^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HL-60, NB4 and U937 cells
Concentration:	125 nM, 250 nM, 500 nM, 1000 nM
Incubation Time:	24 hours, 48 hours, 72 hours
Result:	Dose-dependently inhibited the growth of acute myeloid leukemia (AML) cell lines.

Cell Cycle Analysis^[2]

Cell Line:	HL-60, NB4 and U937 cells
Concentration:	125 nM, 250 nM, 500 nM, 1000 nM
Incubation Time:	24 hours
Result:	Induced a G0/G1 phase accumulation in a concentration-dependent manner.

Western Blot Analysis^[2]

Cell Line:	HL-60 cells
Concentration:	125 nM, 250 nM, 500 nM, 1000 nM
Incubation Time:	24 hours
Result:	Affected the cell-cycle-related molecules (pRb phosphorylation, CDKs, and p21) in HL-60 cells.

Molecular Weight

390.47

Formula

C₂₂H₃₀O₆

CAS No.

60857-08-1

Appearance

Solid

Color

White to off-white

SMILES

CC(O[C@@]12[C@@]([C@@]3([H])[C@]([C@@H](C2)C)([C@@]4([H])[C@](CC(CO)=C3)(C(C(C)=C4)=O)O)O)([H])C1(C)C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (128.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5610 mL	12.8051 mL	25.6102 mL
5 mM	0.5122 mL	2.5610 mL	5.1220 mL
10 mM	0.2561 mL	1.2805 mL	2.5610 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (258 KB) HNMR (259 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Gustafson KR, et al. A nonpromoting phorbol from the samoan medicinal plant Homalanthus nutans inhibits cell killing by HIV-1. J Med Chem. 1992 May 29;35(11):1978-86. [Content Brief]

[2]. Shen X, et al. The protein kinase C agonist prostratin induces differentiation of human myeloid leukemia cells and enhances cellular differentiation by chemotherapeutic agents. Cancer Lett. 2015 Jan 28;356(2 Pt B):686-96. [Content Brief]

[3]. Wang H, et al. Protein kinase D3 is essential for prostratin-activated transcription of integrated HIV-1 provirus promoter via NF-κB signaling pathway. Biomed Res Int. 2014;2014:968027. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5610 mL	12.8051 mL	25.6102 mL	64.0254 mL
	5 mM	0.5122 mL	2.5610 mL	5.1220 mL	12.8051 mL
	10 mM	0.2561 mL	1.2805 mL	2.5610 mL	6.4025 mL
	15 mM	0.1707 mL	0.8537 mL	1.7073 mL	4.2684 mL
	20 mM	0.1281 mL	0.6403 mL	1.2805 mL	3.2013 mL
	25 mM	0.1024 mL	0.5122 mL	1.0244 mL	2.5610 mL
	30 mM	0.0854 mL	0.4268 mL	0.8537 mL	2.1342 mL
	40 mM	0.0640 mL	0.3201 mL	0.6403 mL	1.6006 mL
	50 mM	0.0512 mL	0.2561 mL	0.5122 mL	1.2805 mL
	60 mM	0.0427 mL	0.2134 mL	0.4268 mL	1.0671 mL
	80 mM	0.0320 mL	0.1601 mL	0.3201 mL	0.8003 mL
	100 mM	0.0256 mL	0.1281 mL	0.2561 mL	0.6403 mL

Prostratin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prostratin60857-08-1PKCHIVProtein kinase CHuman immunodeficiency virusterpenoidHIV-1p21Inhibitorinhibitorinhibit

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Prostratin

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Prostratin Related Antibodies

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Complete Stock Solution Preparation Table

Prostratin Related Classifications

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