2. Cell Cycle/DNA Damage Anti-infection
  3. DNA/RNA Synthesis Antibiotic Influenza Virus HIV SARS-CoV
  4. Pyrazofurin

Pyrazofurin  (Synonyms: Pirazofurin)

Cat. No.: HY-122502 Purity: 98.18%
SDS COA Handling Instructions

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Pyrazofurin is an antitumor pyrimidine nucleoside analogue and a orotate-phosphoribosyltransferase inhibitor. Pyrazofurin inhibits cell proliferation and intracellular DNA synthesis by inhibiting uridine 5'-phosphate synthase. Pyrazofurin is also an antibiotic with a broad spectrum of antiviral activity.

For research use only. We do not sell to patients.

Pyrazofurin Chemical Structure

Pyrazofurin Chemical Structure

CAS No. : 30868-30-5

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5 mg In-stock
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Based on 1 publication(s) in Google Scholar

Pyrazofurin Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pyrazofurin is an antitumor pyrimidine nucleoside analogue and a orotate-phosphoribosyltransferase inhibitor. Pyrazofurin inhibits cell proliferation and intracellular DNA synthesis by inhibiting uridine 5'-phosphate synthase. Pyrazofurin is also an antibiotic with a broad spectrum of antiviral activity[1][2][3][4].

In Vitro

Pyrazofurin (75 μM; 52 h) inhibits DNA synthesis in guinea pig lymphocytes treated with Canavagin A(HY-P2149), but is reversed by exogenous uridine[1].
Pyrazofurin (0.01-200 μM; 24-96 h) inhibits the growth of head and neck cancer cell lines HEP-2, UMSCC-14B and UMSCC-14C with the IC50 between 0.06 and 0.37 μM, and decreases the levels of intracellular UTP and CTP and increases the levels of ATP and GTP[2].
Pyrazofurin (0.1-100 μM; 72 h) inhibits parainfluenza type 3, measles, vaccinia, and herpes simplex type 2 viruses in Vero cells with the ED50 between 2.8 and 20 μM [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyrazofurin Related Antibodies
In Vivo

Pyrazofurin (0.25-5 mg/kg; subcutaneous injection; 5 days) has an ameliorative effect in mice infected with Rift Valley fever virus[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rift Valley fever virus-infected female Swiss Webster mice aged 3-4 weeks old[4]
Dosage: 0.25, 2.5 and 10 mg/kg
Administration: Subcutaneous injection (s.c.); 5 days
Result: Increased survival rate and generally prolonged life at 0.25 mg/kg dose.
Was uniformly ineffective at protecting mice from death but did increase the time to death at 2.5 mg/kg dose.
Had toxic to mice at high doses (10 mg/kg).
Molecular Weight

259.22

Formula

C9H13N3O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N)C1=NNC([C@@H]2O[C@@H]([C@H]([C@H]2O)O)CO)=C1O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (192.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (19.29 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8577 mL 19.2886 mL 38.5773 mL
5 mM 0.7715 mL 3.8577 mL 7.7155 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.18%

SDS (251 KB)

COA (250 KB) HNMR (544 KB) RP-HPLC (269 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.8577 mL 19.2886 mL 38.5773 mL 96.4432 mL
5 mM 0.7715 mL 3.8577 mL 7.7155 mL 19.2886 mL
10 mM 0.3858 mL 1.9289 mL 3.8577 mL 9.6443 mL
15 mM 0.2572 mL 1.2859 mL 2.5718 mL 6.4295 mL
DMSO 20 mM 0.1929 mL 0.9644 mL 1.9289 mL 4.8222 mL
25 mM 0.1543 mL 0.7715 mL 1.5431 mL 3.8577 mL
30 mM 0.1286 mL 0.6430 mL 1.2859 mL 3.2148 mL
40 mM 0.0964 mL 0.4822 mL 0.9644 mL 2.4111 mL
50 mM 0.0772 mL 0.3858 mL 0.7715 mL 1.9289 mL
60 mM 0.0643 mL 0.3215 mL 0.6430 mL 1.6074 mL
80 mM 0.0482 mL 0.2411 mL 0.4822 mL 1.2055 mL
100 mM 0.0386 mL 0.1929 mL 0.3858 mL 0.9644 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pyrazofurin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyrazofurin30868-30-5PirazofurinDNA/RNA SynthesisAntibioticInfluenza VirusHIVSARS-CoVHuman immunodeficiency virusSARS coronavirusGuinea pig lymphocyteshead and neck cancer cell linesVero cellsInhibitorinhibitorinhibit

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