RAD51-IN-1

Name: RAD51-IN-1
Brand: MedChemExpress
SKU: HY-122705
Rating: 4.5 (1 reviews)

Cat. No.: HY-122705 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RAD51-IN-1, a derivative of B02, is a potent inhibitor of RAD51. RAD51-IN-1 can be used for cancer research.

For research use only. We do not sell to patients.

RAD51-IN-1 Chemical Structure

CAS No. : 2101739-18-6

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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References
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Description

RAD51-IN-1, a derivative of B02, is a potent inhibitor of RAD51. RAD51-IN-1 can be used for cancer research^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
BT-549	IC₅₀	6.4 μM Compound: 17	Cytotoxicity against human BT549 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human BT549 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
HCC1937	IC₅₀	13.7 μM Compound: 17	Cytotoxicity against human HCC1937 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human HCC1937 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
Hs-578T	IC₅₀	4.2 μM Compound: 17	Cytotoxicity against human Hs578T cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human Hs578T cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MCF-10A	IC₅₀	48.3 μM Compound: 17	Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MDA-MB-231	IC₅₀	6.7 μM Compound: 17	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MDA-MB-436	IC₅₀	4.1 μM Compound: 17	Cytotoxicity against human MDA-MB-436 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MDA-MB-436 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MDA-MB-468	IC₅₀	5.7 μM Compound: 17	Cytotoxicity against human MDA-MB-468 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MDA-MB-468 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
SUM-159-PT	IC₅₀	5.5 μM Compound: 17	Cytotoxicity against human SUM159PT cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human SUM159PT cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]

In Vitro

RAD51 is a vital component of the homologous recombination DNA repair pathway and is overexpressed in drug-resistant cancers, including aggressive triple-negative breast cancer (TNBC)^[1].
RAD51-IN-1 (10 μM) decreases the ratio of RAD51 positive cells/cH2AX positive cells in MDA-MB-231 cell exposure to 6 Gy irradiation^[1].
RAD51-IN-1 (10 μM) significantly inhibits DNA damage induced RAD51 foci formation with 6 Gy irradiation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

373.83

Formula

C₂₂H₁₆ClN₃O

CAS No.

2101739-18-6

Appearance

Solid

Color

Yellow to orange

SMILES

O=C1N(CC2=CC=C(Cl)C=C2)C(/C=C/C3=CC=CN=C3)=NC4=C1C=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (167.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6750 mL	13.3751 mL	26.7501 mL
5 mM	0.5350 mL	2.6750 mL	5.3500 mL
10 mM	0.2675 mL	1.3375 mL	2.6750 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (251 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Ambber Ward, et al. Quinazolinone derivatives as inhibitors of homologous recombinase RAD51. Bioorg Med Chem Lett. 2017 Jul 15;27(14):3096-3100. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6750 mL	13.3751 mL	26.7501 mL	66.8753 mL
	5 mM	0.5350 mL	2.6750 mL	5.3500 mL	13.3751 mL
	10 mM	0.2675 mL	1.3375 mL	2.6750 mL	6.6875 mL
	15 mM	0.1783 mL	0.8917 mL	1.7833 mL	4.4584 mL
	20 mM	0.1338 mL	0.6688 mL	1.3375 mL	3.3438 mL
	25 mM	0.1070 mL	0.5350 mL	1.0700 mL	2.6750 mL
	30 mM	0.0892 mL	0.4458 mL	0.8917 mL	2.2292 mL
	40 mM	0.0669 mL	0.3344 mL	0.6688 mL	1.6719 mL
	50 mM	0.0535 mL	0.2675 mL	0.5350 mL	1.3375 mL
	60 mM	0.0446 mL	0.2229 mL	0.4458 mL	1.1146 mL
	80 mM	0.0334 mL	0.1672 mL	0.3344 mL	0.8359 mL
	100 mM	0.0268 mL	0.1338 mL	0.2675 mL	0.6688 mL

RAD51-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RAD51-IN-12101739-18-6RAD51tumor cellDNA damagechemotherapycancerInhibitorinhibitorinhibit

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RAD51-IN-1

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RAD51-IN-1 Related Antibodies

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RAD51-IN-1 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

RAD51-IN-1 Related Classifications

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