Calcitonin/CALCA Protein, Mouse (HEK293, Fc)

The Calcitonin/CALCA protein induces vasodilation in different blood vessels, including the coronary, cerebral, and systemic vasculature. It is highly concentrated in the central nervous system, suggesting a role as a neurotransmitter or neuromodulator. Moreover, it increases platelet cAMP levels, possibly affecting platelet function. Calcitonin/CALCA Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Calcitonin/CALCA protein, expressed by HEK293 , with C-hFc labeled tag.

The Calcitonin/CALCA protein is known to induce vasodilation, affecting various types of blood vessels such as the coronary, cerebral, and systemic vasculature. Its high concentration in the central nervous system suggests its involvement as a neurotransmitter or neuromodulator. Additionally, it has been observed to increase platelet cyclic adenosine monophosphate (cAMP) levels, indicating its potential impact on platelet function.

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 4.133 µg/mL.

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  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 4.133 μg/mL, corresponding to a specific activity is 241.9 units/mg.

Mouse

HEK293

C-hFc

Q99JA0 (V26-F119)

12310  [NCBI]

VPLRSILESSPGMATLSEEEVRLLAALVQDYMQMKARELEQEEEQEAEGSSVTAQKRSCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSEAF

Approximately 42-48 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.