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GltI/YbeJ Protein, E.coli (His-SUMO)

Cat. No.: HY-P71460
Handling Instructions

The GltI/YbeJ protein is a key component of the ABC transporter complex GltIJKL and is essential for active transport of glutamate and aspartate. As the solute-binding protein in the complex, GltI binds glutamate and aspartate. GltI/YbeJ Protein, E.coli (His-SUMO) is the recombinant E. coli-derived GltI/YbeJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

For research use only. We do not sell to patients.

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Description

The GltI/YbeJ protein is a key component of the ABC transporter complex GltIJKL and is essential for active transport of glutamate and aspartate. As the solute-binding protein in the complex, GltI binds glutamate and aspartate. GltI/YbeJ Protein, E.coli (His-SUMO) is the recombinant E. coli-derived GltI/YbeJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Background

GltI/YbeJ protein is an integral component of the ABC transporter complex GltIJKL, crucial for the active uptake of glutamate and aspartate. Operating as a solute-binding protein within the complex, GltI plays a pivotal role in binding both glutamate and aspartate. This complex comprises two ATP-binding proteins (GltL), two transmembrane proteins (GltJ and GltK), and the solute-binding protein GltI. Together, these components orchestrate the efficient transport of glutamate and aspartate across the cell membrane, highlighting the essential function of GltI/YbeJ in this process.

Species

E.coli

Source

E. coli

Tag

N-His;N-SUMO

Accession

P37902 (D23-N302)

Gene ID

946938  [NCBI]

Molecular Construction
N-term
6*His-SUMO
GltI (D23-N302)
Accession # P37902
C-term
Synonyms
gltI; ybeJ; yzzK; b0655; JW5092Glutamate/aspartate import solute-binding protein
AA Sequence

DDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKALN

Molecular Weight

Approximately 47.2 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

GltI/YbeJ Protein, E.coli (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GltI/YbeJ Protein, E.coli (His-SUMO)
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HY-P71460
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