1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. GM-CSF
  5. GM-CSF Protein, Human (P.pastoris)

GM-CSF Protein is a hematopoietic growth factor and immunomodulator. By binding to specific receptors on the surface of target cells, GM-CSF Protein activates intracellular signaling pathways to regulate cell proliferation, differentiation, maturation, and function. GM-CSF Protein plays an important role in the hematopoietic process and the immune system. GM-CSF Protein, Human (P.pastoris) is a recombinant GM-CSF Protein expressed by P. pastoris without a tag.

For research use only. We do not sell to patients.

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10 μg USD 142 In-stock
50 μg USD 399 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

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Description

GM-CSF Protein is a hematopoietic growth factor and immunomodulator. By binding to specific receptors on the surface of target cells, GM-CSF Protein activates intracellular signaling pathways to regulate cell proliferation, differentiation, maturation, and function. GM-CSF Protein plays an important role in the hematopoietic process and the immune system. GM-CSF Protein, Human (P.pastoris) is a recombinant GM-CSF Protein expressed by P. pastoris without a tag[1][2][3][4].

Background

GM-CSF is a cytokine secreted by various cells (such as T cells, monocytes, endothelial cells, etc.), playing an important role in the immune system and hematopoietic process. GM-CSF can stimulate the proliferation and differentiation of precursor cells of granulocytes (such as neutrophils and eosinophils) and macrophages in the bone marrow, promoting the production of mature blood cells. GM-CSF can also activate mature granulocytes and macrophages, enhancing their phagocytic ability, bactericidal activity, and antigen-presenting function, and participating in innate and adaptive immune responses. In addition, GM-CSF also has pro-inflammatory activity[1][2][3][4].

In Vitro

GM-CSF Protein (Human; 1% v/v; 7-10 days) can promote the proliferation of acute myeloid leukemia colony-forming cells (AML-CFU)[5].

In Vivo

GM-CSF Protein (Human; 50 U/min/kg; continuous intravenous/subcutaneous infusion; 7-28 days) significantly increases white blood cell counts and bone marrow cellularity in healthy cynomolgous macaques and pancytopenic immunodeficient rhesus macaques, with indices returning to normal after drug withdrawal[6].

Biological Activity

The ED50 is ≤0.2 ng/mL as measured by TF-1 cells, corresponding to a specific activity of ≥5.0 × 106 units/mg.

Species

Human

Source

P. pastoris

Tag

Tag Free

Accession

P04141 (A18-E144)

Gene ID
Molecular Construction
N-term
GM-CSF (A18-E144)
Accession # P04141
C-term
Synonyms
rHuGM-CSF; CSF-2; MGI-1GM; Pluripoietin-alpha; Molgramostin; Sargramostim
AA Sequence

MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Molecular Weight

24-35 kDa

Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

GM-CSF Protein, Human (P.pastoris) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
GM-CSF Protein, Human (P.pastoris)
Cat. No.:
HY-P7016B
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