1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. FGF Family
  4. Fibroblast Growth Factor
  5. Fibroblast Growth Factor 7 (FGF-7)
  6. KGF/FGF-7 Protein, Human

KGF/FGF-7 Protein is an epithelial cell-specific mitogen secreted by normal stromal fibroblasts. KGF/FGF-7 Protein activates plasminogen activator (PA) activity to promote extracellular matrix degradation. KGF/FGF-7 Protein, Human has activities such as promoting the proliferation and differentiation of epithelial cells (such as keratinocytes, thymic epithelial cells), repairing tissue damage (such as intestinal ischemia/reperfusion injury, bone defects), and regulating immune function (such as improving thymus function in aged mice). KGF/FGF-7 Protein, Human is a recombinant KGF/FGF-7 protein expressed by E. coli without a tag.

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  • Biological Activity

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Description

KGF/FGF-7 Protein is an epithelial cell-specific mitogen secreted by normal stromal fibroblasts. KGF/FGF-7 Protein activates plasminogen activator (PA) activity to promote extracellular matrix degradation. KGF/FGF-7 Protein, Human has activities such as promoting the proliferation and differentiation of epithelial cells (such as keratinocytes, thymic epithelial cells), repairing tissue damage (such as intestinal ischemia/reperfusion injury, bone defects), and regulating immune function (such as improving thymus function in aged mice). KGF/FGF-7 Protein, Human is a recombinant KGF/FGF-7 protein expressed by E. coli without a tag.

Background

KGF/FGF-7 Protein belongs to the fibroblast growth factor family[1]. KGF/FGF-7 Protein is an epithelial cell-specific mitogen secreted by normal stromal fibroblasts[1]. KGF/FGF-7 Protein stimulates epithelial cell proliferation and migration and regulates differentiation processes by binding to specific receptors on the surface of epithelial cells. For example, under high calcium conditions, it promotes keratinocytes to express early (K1) and late (filagrin) differentiation markers, and activates plasminogen activator (PA) activity to promote extracellular matrix degradation[1][3]. KGF/FGF-7 Protein is expressed in mesenchymal cells of various tissues such as skin, gastrointestinal tract, lung, and thymus, and acts on epithelial cells through paracrine effects[1][2][5][6][7]. KGF/FGF-7 Protein, Human has activities such as promoting the proliferation and differentiation of epithelial cells (such as keratinocytes and thymic epithelial cells), repairing tissue damage (such as intestinal ischemia/reperfusion injury and bone defects), and regulating immune function (such as improving thymus function in aged mice). For example, it stimulates keratinocyte migration and PA activity in wound repair, and promotes the expression of osteogenic markers and new bone formation in bone defect models[1][3][4][5][6][7].

In Vitro

KGF/FGF-7 Protein, Human (10 ng/mL; 4 days) promotes human keratinocyte proliferation with an effect comparable to that of EGF[1].
KGF/FGF-7 Protein, Human (20 ng/mL; 6-48 h) induces increased SCF mRNA and protein secretion in human HaCaT keratinocytes[2].
KGF/FGF-7 Protein, Human (1 nM; 16 h) significantly stimulates human keratinocyte migration and urokinase-type plasminogen activator (uPA) activity[3].
KGF/FGF-7 Protein, Human (25-200 ng/mL; 48 h for migration test) stimulates migration of rat bone marrow stromal cells (BMSCs) and increases the expression of SDF-1 and CXCR4[4].

In Vivo

KGF/FGF-7 Protein, Human (5 mg/kg; s.c.; 3 days; before transplantation) reduces GVHD mortality and improves liver, lung and other organ damage in the B10.BR mouse allogeneic bone marrow transplantation model[5].
KGF/FGF-7 Protein, Human (5 mg/kg/d; i.p.; 5 days; before surgery) increases intestinal wet weight, reduces epithelial apoptosis, and restores tight junction protein distribution in the intestinal ischemia/reperfusion model of C57BL/6J mice[6].
KGF/FGF-7 Protein, Human (5 mg/kg/d; s.c.; 3 days) induces thymic regeneration, increases thymocyte number and naive T cell output in C57BL/6J mice[7].

Biological Activity

Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells.The ED50 for this effect is ≤110.3 ng/mL, corresponding to a specific activity is ≥9.07×103 U/mg.

  • Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells.The ED50 for this effect is 15.20 ng/mL, corresponding to a specific activity is 6.6×104 U/mg.
Species

Human

Source

E. coli

Tag

Tag Free

Accession

P21781-1 (C32-T194)

Gene ID
Molecular Construction
N-term
FGF-7 (C32-T194)
Accession # P21781
C-term
Synonyms
Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF
AA Sequence

CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT

Molecular Weight

Approximately 17.6-20 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1mM EDTA, 5% Trehalose, 0.02% Tween 80, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCL, pH 7.4 or PBS or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80 or 20 mM PB, 500 mM NaCl, pH 8.0, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
KGF/FGF-7 Protein, Human
Cat. No.:
HY-P70597
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