Seselin

Name: Seselin
Brand: MedChemExpress
SKU: HY-W505771
Rating: 4.5 (1 reviews)

Cat. No.: HY-W505771 Purity: 98.48%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Seselin is an anticancer, antinociceptive, anti-inflammatory and antifungal agent. Seselin is orally active.

For research use only. We do not sell to patients.

Seselin Chemical Structure

CAS No. : 523-59-1

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
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100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Seselin is an anticancer, antinociceptive, anti-inflammatory and antifungal agent. Seselin is orally active^[1]^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 20 μg/mL Compound: 16	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 29975532]
HepG2	IC₅₀	> 20 μg/mL Compound: 16	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 29975532]
MDA-MB-231	IC₅₀	> 20 μg/mL Compound: 16	Cytotoxicity against human MDA-MB-231 cells by MTT assay Cytotoxicity against human MDA-MB-231 cells by MTT assay	[PMID: 29975532]
Neutrophil	IC₅₀	> 10 μM Compound: 16	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29975532]
Neutrophil	IC₅₀	4.7 μM Compound: 16	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochro Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochro	[PMID: 29975532]
Vero	IC₅₀	12 μg/mL Compound: 10	Cytotoxicity against african green monkey Vero cells after 7 days by XTT assay Cytotoxicity against african green monkey Vero cells after 7 days by XTT assay	[PMID: 8021652]

In Vitro

Seselin shows cytotoxic effects with ED₅₀ of 8.66 and 9.94 μg/mL against P-388 and HT-29 cells, respectively^[1].
Seselin (5-20 μM; 0.5-24 h) inhibits cytokine output from macrophages stimulated by LPS and IFN-γ dose- and time-dependently^[3].
Seselin (5-20 μM; 12 h) inhibits the expression of proinflammatory macrophage markers (iNOS, phagocytosis, CD11c) in BMDMs^[3].
Seselin (5-20 μM; 0.5-6 h) blocks the STAT1 signalling pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[3]

Cell Line:	Bone marrow-derived macrophages (BMDMs)
Concentration:	5, 10 and 20 μM
Incubation Time:	6 h
Result:	Reduced the mRNA for cytokines (IL-1β, IL-6, Tnf-α and IL-23) and chemokines (Ccl3, Ccl7, Cxcl9 and Cxcl11) concentration-dependently in BMDMs.

Western Blot Analysis^[3]

Cell Line:	BMDMs
Concentration:	5, 10 and 20 μM
Incubation Time:	0.5, 1.5, 3 and 6 h
Result:	Suppressed expression of p-STAT1 and p-p65 both concentration and time dependently.

In Vivo

Seselin (0.5-40.5 mg/kg; s.c.; once) shows peripheral anti-inflammatory and antinociceptive activities in mice^[2].
Seselin (3-30 mg/kg; i.g.; once) ameliorates sepsis induced by caecal ligation and puncture in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Swiss mice^[2]
Dosage:	0.5, 4.5 or 40.5 mg/kg
Administration:	Subcutaneous injection; once
Result:	Inhibited the writhing response induced by acetic acid in a significant and dose-dependent manner, by 19.5%, 26.2% and 41.4% at dose of 0.5, 4.5 or 40.5 mg/kg, respectively. Elicited a significant inhibition of formalin response during the second phase (inflammatory), by 90.3%, 97.8% and 95.3%, respectively.

Animal Model:	C57BL/6 mice, caecal ligation and puncture (CLP) induced sepsis model^[3]
Dosage:	3, 10 and 30 mg/kg
Administration:	Intragastric administration, once
Result:	Ameliorated lung injury and decreased JAK2 phosphorylation level in lung tissue during sepsis. Reduced the immune cell counts in BALF induced by CLP.

Molecular Weight

228.24

Formula

C₁₄H₁₂O₃

CAS No.

523-59-1

Appearance

Solid

Color

White to off-white

SMILES

CC1(C=CC2=C(O1)C=CC3=C2OC(=O)C=C3)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (438.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3814 mL	21.9068 mL	43.8135 mL
5 mM	0.8763 mL	4.3814 mL	8.7627 mL
10 mM	0.4381 mL	2.1907 mL	4.3814 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.48%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (253 KB) HNMR (100 KB) LCMS (286 KB)

Handling Instructions (2659 KB)

References

[1]. Chou HC, et al. Cytotoxic and anti-platelet aggregation constituents from the root wood of Melicope semecarpifolia. Planta Med. 2005 Nov;71(11):1078-81. [Content Brief]

[2]. Lima V, et al. Antinociceptive activity of the pyranocoumarin seselin in mice. Fitoterapia. 2006 Dec;77(7-8):574-8.Lima V, et al. Antinociceptive activity of the pyranocoumarin seselin in mice. Fitoterapia. 2006 Dec;77(7-8):574-8.Lima V, et al. Antinociceptive activity of the pyranocoumarin seselin in mice. Fitoterapia. 2006 Dec;77(7-8):574-8. [Content Brief]

[3]. Feng L, et al. Seselin ameliorates inflammation via targeting Jak2 to suppress the proinflammatory phenotype of macrophages. Br J Pharmacol. 2019 Jan;176(2):317-333. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3814 mL	21.9068 mL	43.8135 mL	109.5338 mL
	5 mM	0.8763 mL	4.3814 mL	8.7627 mL	21.9068 mL
	10 mM	0.4381 mL	2.1907 mL	4.3814 mL	10.9534 mL
	15 mM	0.2921 mL	1.4605 mL	2.9209 mL	7.3023 mL
	20 mM	0.2191 mL	1.0953 mL	2.1907 mL	5.4767 mL
	25 mM	0.1753 mL	0.8763 mL	1.7525 mL	4.3814 mL
	30 mM	0.1460 mL	0.7302 mL	1.4605 mL	3.6511 mL
	40 mM	0.1095 mL	0.5477 mL	1.0953 mL	2.7383 mL
	50 mM	0.0876 mL	0.4381 mL	0.8763 mL	2.1907 mL
	60 mM	0.0730 mL	0.3651 mL	0.7302 mL	1.8256 mL
	80 mM	0.0548 mL	0.2738 mL	0.5477 mL	1.3692 mL
	100 mM	0.0438 mL	0.2191 mL	0.4381 mL	1.0953 mL

Seselin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Seselin523-59-1FungalP-388HT-29BMDMsiNOSphagocytosisCD11cCcl3Ccl7Cxcl9Cxcl11IL-1βIL-6Tnf-αIL-23Inhibitorinhibitorinhibit

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