SKI-349

Cat. No.: HY-162143 Purity: 99.58%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SKI-349 is a dual-targeted inhibitor of sphingosine kinase 1/2 (SPHK1/2) and microtubule assembly (MDA). SKI-349 has anticancer activity. SKI-349 can inhibit the vitality, invasion, and AKT/mTOR signaling pathway of liver cells.

For research use only. We do not sell to patients.

SKI-349 Chemical Structure

CAS No. : 727686-80-8

Size	Price	Stock	Quantity
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	IC₅₀	10 nM Compound: 5p	Antiproliferative activity against human CEM cells after 2 days by coulter counter method Antiproliferative activity against human CEM cells after 2 days by coulter counter method	[PMID: 19663386]
FM3A	IC₅₀	71 nM Compound: 5p	Antiproliferative activity against mouse FM3A cells after 2 days by coulter counter method Antiproliferative activity against mouse FM3A cells after 2 days by coulter counter method	[PMID: 19663386]
HeLa	IC₅₀	52 nM Compound: 5p	Antiproliferative activity against human HeLa cells after 2 days by coulter counter method Antiproliferative activity against human HeLa cells after 2 days by coulter counter method	[PMID: 19663386]
HL-60	IC₅₀	22 nM Compound: SKI-349	Cytotoxicity against human HL-60 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HL-60 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32736077]
L1210	IC₅₀	38 nM Compound: 5p	Antiproliferative activity against mouse L1210 cells after 2 days by coulter counter method Antiproliferative activity against mouse L1210 cells after 2 days by coulter counter method	[PMID: 19663386]
MOLT-4	IC₅₀	37 nM Compound: 5p	Antiproliferative activity against human MOLT4 cells after 2 days by coulter counter method Antiproliferative activity against human MOLT4 cells after 2 days by coulter counter method	[PMID: 19663386]

Molecular Weight

385.44

Formula

C₁₉H₁₉N₃O₄S

CAS No.

727686-80-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(N)N=C(NC2=CC=C(C=C2)OC)S1)C3=CC=C(C(OC)=C3)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (324.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5944 mL	12.9722 mL	25.9444 mL
5 mM	0.5189 mL	2.5944 mL	5.1889 mL
10 mM	0.2594 mL	1.2972 mL	2.5944 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (251 KB) HNMR (262 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Hengst JA, et al. Development of SKI-349, a dual-targeted inhibitor of sphingosine kinase and microtubule polymerization. Bioorg Med Chem Lett. 2020 Oct 15;30(20):127453. [Content Brief]

[2]. Chen L, et al. SKI-349, a Sphingosine Kinases 1/2 Inhibitor, Suppresses Cell Viability, Invasion, and AKT/mTOR Signaling Pathway, and Shows Synergistic Cytotoxic Effects with Sorafenib in Hepatocellular Carcinoma. Tohoku J Exp Med. 2023 Dec 21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5944 mL	12.9722 mL	25.9444 mL	64.8609 mL
	5 mM	0.5189 mL	2.5944 mL	5.1889 mL	12.9722 mL
	10 mM	0.2594 mL	1.2972 mL	2.5944 mL	6.4861 mL
	15 mM	0.1730 mL	0.8648 mL	1.7296 mL	4.3241 mL
	20 mM	0.1297 mL	0.6486 mL	1.2972 mL	3.2430 mL
	25 mM	0.1038 mL	0.5189 mL	1.0378 mL	2.5944 mL
	30 mM	0.0865 mL	0.4324 mL	0.8648 mL	2.1620 mL
	40 mM	0.0649 mL	0.3243 mL	0.6486 mL	1.6215 mL
	50 mM	0.0519 mL	0.2594 mL	0.5189 mL	1.2972 mL
	60 mM	0.0432 mL	0.2162 mL	0.4324 mL	1.0810 mL
	80 mM	0.0324 mL	0.1622 mL	0.3243 mL	0.8108 mL
	100 mM	0.0259 mL	0.1297 mL	0.2594 mL	0.6486 mL

SKI-349 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SKI-349727686-80-8SKI349SKI 349SphKAktmTORSphingosine kinasePKBProtein kinase BMammalian target of RapamycinHepatocellular carcinoma (HCC)liver cellsanticancerAKT/mTORInhibitorinhibitorinhibit

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SKI-349

Customer Review

SKI-349 Related Antibodies

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•Related Small Molecules:

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View All SphK Isoform Specific Products:

View All Akt Isoform Specific Products:

View All mTOR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SKI-349 Related Classifications

Keywords:

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Customer Review

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SAFETY DATA SHEET (SDS)

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