SKI-I

Name: SKI-I
Brand: MedChemExpress
SKU: HY-115735
Rating: 4.5 (1 reviews)

Cat. No.: HY-115735 Purity: 99.14%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SKI-I is a potent and selective inhibitor of human sphingosine kinase (SK), with an IC₅₀ of 1.2 μM for ST-hSK. SKI-I also inhibits hERK2 (IC₅₀=11 μM). SKI-I induces apoptosis in tumor cell lines.

For research use only. We do not sell to patients.

SKI-I Chemical Structure

CAS No. : 306301-68-8

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1850	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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SphK1 SphK2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 1.2 μM (ST-hSK); 11 μM (hERK2)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	3.8 μM Compound: 4, SKI-I	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 20598402]
HeLa	IC₅₀	1 μM Compound: 4, SKI-I	Cytotoxicity against human HeLa cells after 72 hrs by SRB assay Cytotoxicity against human HeLa cells after 72 hrs by SRB assay	[PMID: 20598402]
MCF-10A	IC₅₀	4.1 μM Compound: 4, SKI-I	Cytotoxicity against human MCF10A cells after 72 hrs by SRB assay Cytotoxicity against human MCF10A cells after 72 hrs by SRB assay	[PMID: 20598402]
MDA-MB-231	IC₅₀	4.4 μM Compound: 4, SKI-I	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by SRB assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by SRB assay	[PMID: 20598402]
MEF	IC₅₀	5 μM Compound: 4, SKI-I	Cytotoxicity against MEF after 72 hrs by SRB assay Cytotoxicity against MEF after 72 hrs by SRB assay	[PMID: 20598402]
MIA PaCa-2	IC₅₀	4.8 μM Compound: 4, SKI-I	Cytotoxicity against human MIAPaCa2 cells after 72 hrs by SRB assay Cytotoxicity against human MIAPaCa2 cells after 72 hrs by SRB assay	[PMID: 20598402]
NCI-H226	IC₅₀	5.7 μM Compound: 4, SKI-I	Cytotoxicity against human NCI-H226 cells after 72 hrs by SRB assay Cytotoxicity against human NCI-H226 cells after 72 hrs by SRB assay	[PMID: 20598402]
NCI-H441	IC₅₀	2 μM Compound: 4, SKI-I	Cytotoxicity against human H441 cells after 72 hrs by SRB assay Cytotoxicity against human H441 cells after 72 hrs by SRB assay	[PMID: 20598402]
NCI-H460	IC₅₀	3.2 μM Compound: 4, SKI-I	Cytotoxicity against human H460 cells after 72 hrs by SRB assay Cytotoxicity against human H460 cells after 72 hrs by SRB assay	[PMID: 20598402]
U-87MG ATCC	IC₅₀	1 μM Compound: 4, SKI-I	Cytotoxicity against human U87MG cells after 72 hrs by SRB assay Cytotoxicity against human U87MG cells after 72 hrs by SRB assay	[PMID: 20598402]

In Vitro

SKI-I (Compound I; 5 μg/mL) inhibits SK activity by 99%^[1].
SKI-I (10 μM; 24 h) induces the apoptosis of T24 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

406.44

Formula

C₂₅H₁₈N₄O₂

CAS No.

306301-68-8

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=NNC(C2=CC=C3C=CC=CC3=C2)=C1)N/N=C/C4=C5C=CC=CC5=CC=C4O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (246.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4604 mL	12.3019 mL	24.6039 mL
5 mM	0.4921 mL	2.4604 mL	4.9208 mL
10 mM	0.2460 mL	1.2302 mL	2.4604 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.14%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (251 KB) HNMR (271 KB) RP-HPLC (254 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. French KJ, et, al. Discovery and evaluation of inhibitors of human sphingosine kinase. Cancer Res. 2003 Sep 15;63(18):5962-9. [Content Brief]

[2]. Sharma AK, et, al. Synthesis and bioactivity of sphingosine kinase inhibitors and their novel aspirinyl conjugated analogs. Eur J Med Chem. 2010 Sep;45(9):4149-56. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4604 mL	12.3019 mL	24.6039 mL	61.5097 mL
	5 mM	0.4921 mL	2.4604 mL	4.9208 mL	12.3019 mL
	10 mM	0.2460 mL	1.2302 mL	2.4604 mL	6.1510 mL
	15 mM	0.1640 mL	0.8201 mL	1.6403 mL	4.1006 mL
	20 mM	0.1230 mL	0.6151 mL	1.2302 mL	3.0755 mL
	25 mM	0.0984 mL	0.4921 mL	0.9842 mL	2.4604 mL
	30 mM	0.0820 mL	0.4101 mL	0.8201 mL	2.0503 mL
	40 mM	0.0615 mL	0.3075 mL	0.6151 mL	1.5377 mL
	50 mM	0.0492 mL	0.2460 mL	0.4921 mL	1.2302 mL
	60 mM	0.0410 mL	0.2050 mL	0.4101 mL	1.0252 mL
	80 mM	0.0308 mL	0.1538 mL	0.3075 mL	0.7689 mL
	100 mM	0.0246 mL	0.1230 mL	0.2460 mL	0.6151 mL

SKI-I Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SKI-I306301-68-8SphKApoptosisSphingosine kinasesphingosinekinaseSKST-hSKhERK2apoptosiscancerInhibitorinhibitorinhibit

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SKI-I

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SKI-I Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All SphK Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

SKI-I Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

SKI-I Related Classifications

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Customer Review

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