SMYD3-IN-1

Cat. No.: HY-128352 Purity: 98.01%: FAQs
Data Sheet SDS COA Handling Instructions

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SMYD3-IN-1 (compound 29) is an irreversible and selective inhibitor of SMYD3 (SET and MYND domain containing 3), with an IC₅₀ of 11.7 nM.

For research use only. We do not sell to patients.

SMYD3-IN-1 Chemical Structure

CAS No. : 2095160-79-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 480	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 480	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 430	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1400	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2300	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3700	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms

EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

Biological Activity
Purity & Documentation
References
Customer Review

Description

SMYD3-IN-1 (compound 29) is an irreversible and selective inhibitor of SMYD3 (SET and MYND domain containing 3), with an IC₅₀ of 11.7 nM^[1].

IC₅₀ & Target^[1]

SMYD3

11.7 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	GI₅₀	1.04 μM Compound: 29	Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of anchorage-independent colony formation in 3D culture cells incubated for 1 to 2 weeks by thiazolyl blue tetrazolium bromide based Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of anchorage-independent colony formation in 3D culture cells incubated for 1 to 2 weeks by thiazolyl blue tetrazolium bromide based	[PMID: 31223458]
HepG2	GI₅₀	17.69 μM Compound: 29	Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of cell proliferation incubated for 3 days by CellTiter-Glo luminescent assay Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of cell proliferation incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31223458]

Molecular Weight

507.02

Formula

C₂₈H₃₁ClN₄O₃

CAS No.

2095160-79-3

Appearance

Solid

SMILES

O=C(N1[C@H](C)CN(C(C2=CC=C3C(Cl)=CC(C4=CC=C(C5(N)CC5)C=C4)=NC3=C2)=O)CC1)OCCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (197.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9723 mL	9.8615 mL	19.7231 mL
5 mM	0.3945 mL	1.9723 mL	3.9446 mL
10 mM	0.1972 mL	0.9862 mL	1.9723 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.01%

Select Batch:

Data Sheet (268 KB) SDS (251 KB)

COA (246 KB) HNMR (163 KB) RP-HPLC (163 KB) LCMS (335 KB)

Handling Instructions (2659 KB)

References

[1]. Huang C, et al. Discovery of Irreversible Inhibitors Targeting Histone Methyltransferase, SMYD3. ACS Med Chem Lett. 2019 May 23;10(6):978-984. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9723 mL	9.8615 mL	19.7231 mL	49.3077 mL
	5 mM	0.3945 mL	1.9723 mL	3.9446 mL	9.8615 mL
	10 mM	0.1972 mL	0.9862 mL	1.9723 mL	4.9308 mL
	15 mM	0.1315 mL	0.6574 mL	1.3149 mL	3.2872 mL
	20 mM	0.0986 mL	0.4931 mL	0.9862 mL	2.4654 mL
	25 mM	0.0789 mL	0.3945 mL	0.7889 mL	1.9723 mL
	30 mM	0.0657 mL	0.3287 mL	0.6574 mL	1.6436 mL
	40 mM	0.0493 mL	0.2465 mL	0.4931 mL	1.2327 mL
	50 mM	0.0394 mL	0.1972 mL	0.3945 mL	0.9862 mL
	60 mM	0.0329 mL	0.1644 mL	0.3287 mL	0.8218 mL
	80 mM	0.0247 mL	0.1233 mL	0.2465 mL	0.6163 mL
	100 mM	0.0197 mL	0.0986 mL	0.1972 mL	0.4931 mL

SMYD3-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMYD3-IN-12095160-79-3Histone MethyltransferaseInhibitorinhibitorinhibit

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SMYD3-IN-1

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SMYD3-IN-1 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Methyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

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Dilution Calculator

Complete Stock Solution Preparation Table

SMYD3-IN-1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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