Stafia-1-dipivaloyloxymethyl ester

Name: Stafia-1-dipivaloyloxymethyl ester
Brand: MedChemExpress
SKU: HY-136568
Rating: 4.5 (1 reviews)

Cat. No.: HY-136568 Purity: 98.31%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Stafia-1-dipivaloyloxymethyl ester (compound 27, 0-200 μM) decreases pSTAT5a expression significantly, and has no obvious inhibition on pSTAT5b.

For research use only. We do not sell to patients.

Stafia-1-dipivaloyloxymethyl ester Chemical Structure

CAS No. : 2582755-72-2

Size	Price	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 495	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 495	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Stafia-1-dipivaloyloxymethyl ester (compound 27, 0-200 μM) decreases pSTAT5a expression significantly, and has no obvious inhibition on pSTAT5b^[1].

IC₅₀ & Target^[1]

STAT5a

In Vitro

Stafia-1-dipivaloyloxymethyl ester (compound 27, 0-200 μM) decreases pSTAT5a expression significantly, and has no obvious inhibition on pSTAT5b^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	STAT5a-GFP-transfected K562 cells.
Concentration:	0-200 μM.
Incubation Time:
Result:	Decreased pSTAT5a expression.

Molecular Weight

750.74

Formula

C₃₇H₄₈FO₁₃P

CAS No.

2582755-72-2

Appearance

Oil

Color

White to light yellow

SMILES

O=C(C(C)(C)C)OCOP(OCOC(C(C)(C)C)=O)(C(C1=CC(C2=CC(OC)=C(OC)C(OC)=C2)=CC(C3=CC(OC)=C(C(OC)=C3)OC)=C1)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (133.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3320 mL	6.6601 mL	13.3202 mL
5 mM	0.2664 mL	1.3320 mL	2.6640 mL
10 mM	0.1332 mL	0.6660 mL	1.3320 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.31%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (256 KB) HNMR (300 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Kalaiselvi Natarajan, et al. Stafia-1: A STAT5a-Selective Inhibitor Developed via Docking-Based Screening of in Silico O-Phosphorylated Fragments. Chemistry. 2020 Jan 2;26(1):148-154. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3320 mL	6.6601 mL	13.3202 mL	33.3005 mL
	5 mM	0.2664 mL	1.3320 mL	2.6640 mL	6.6601 mL
	10 mM	0.1332 mL	0.6660 mL	1.3320 mL	3.3300 mL
	15 mM	0.0888 mL	0.4440 mL	0.8880 mL	2.2200 mL
	20 mM	0.0666 mL	0.3330 mL	0.6660 mL	1.6650 mL
	25 mM	0.0533 mL	0.2664 mL	0.5328 mL	1.3320 mL
	30 mM	0.0444 mL	0.2220 mL	0.4440 mL	1.1100 mL
	40 mM	0.0333 mL	0.1665 mL	0.3330 mL	0.8325 mL
	50 mM	0.0266 mL	0.1332 mL	0.2664 mL	0.6660 mL
	60 mM	0.0222 mL	0.1110 mL	0.2220 mL	0.5550 mL
	80 mM	0.0167 mL	0.0833 mL	0.1665 mL	0.4163 mL
	100 mM	0.0133 mL	0.0666 mL	0.1332 mL	0.3330 mL