TJ191

Name: TJ191
Brand: MedChemExpress
SKU: HY-120075
Rating: 4.5 (1 reviews)

Cat. No.: HY-120075 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TJ191 is a potent and specific anti-cancer agent that targets low TβRIII-expressing malignant T-cell leukemia/lymphoma cells. TJ191 has no affects on the proliferation of other cancer cells or normal fibroblasts or immune cells. TJ191 can be used for cancer research.

For research use only. We do not sell to patients.

TJ191 Chemical Structure

CAS No. : 1522415-97-9

Size	Price	Stock	Quantity
5 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CAKI-1	IC₅₀	0.54 μM Compound: 3j	Cytotoxicity against human Caki1 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human Caki1 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
CCRF-CEM	IC₅₀	0.18 μM Compound: 3j	Cytotoxicity against human CEM cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human CEM cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
HeLa	IC₅₀	81 μM Compound: 3j	Cytotoxicity against human HeLa cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human HeLa cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
Huh-7	IC₅₀	0.34 μM Compound: 3j	Cytotoxicity against human HuH7 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human HuH7 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
MOLT-4	IC₅₀	0.057 μM Compound: 3j	Cytotoxicity against human MOLT4 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human MOLT4 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
PBMC	IC₅₀	> 50 μM Compound: 3j	Cytotoxicity against human PBMC cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human PBMC cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
PC-3	IC₅₀	0.62 μM Compound: 3j	Cytotoxicity against human PC3 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human PC3 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
Raji	IC₅₀	105 μM Compound: 3j	Cytotoxicity against human Raji cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human Raji cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]

In Vitro

TJ191(0-100 µM; 24 hours) exhibits pronounced anti-proliferative activity in malignant cell lines, the IC₅₀ values are 0.13 µM, 0.13±0.08 µM, 0.26±0.19 µM, 0.22±0.11 µM, 1.5±0.02 µM, 0.32±0.086 µM, 3.1±0.5 µM, 0.26±0.16 µM in CEM, JURKAT, MOLT-3, MOLT-4, SUP-T1, MT-2, C8166 and HSB-2 cells, respectively. But has no effects in HUT-78 (IC₅₀=17±10 µM) and MT-4 (IC₅₀=47 ± 5 µM) cells^[1].
TJ191 (0.1-3 µM; 8 or 24 hours) induces CEM cell apoptosis in a concentration- and time-dependent manner. Even at 0.3 µM, TJ191 induces the maximum apoptotic rate of 80% after 24 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Malignant T-cell leukemia/lymphoma cells
Concentration:	0.01 µM, 0.1 µM, 1 µM, 10 µM, 100 µM
Incubation Time:	24 hours
Result:	Exhibited inhibitory effects in drug-sensitive cells with IC₅₀ ranging 0.13 µM to 3.1 µM.

Apoptosis Analysis^[1]

Cell Line:	CEM cell line
Concentration:	0.1-3 µM
Incubation Time:	8 or 24 hours
Result:	Led to cell apoptosis.

Molecular Weight

255.38

Formula

C₁₃H₂₁NO₂S

CAS No.

1522415-97-9

Appearance

Solid

Color

Light yellow to khaki

SMILES

O=C(C1=C(N)SC(CCCCCCC)=C1)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (391.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9157 mL	19.5787 mL	39.1573 mL
5 mM	0.7831 mL	3.9157 mL	7.8315 mL
10 mM	0.3916 mL	1.9579 mL	3.9157 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.79 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (244 KB) HNMR (260 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. Ahmed El-Gazzar, et al. 2-Amino-3-methylcarboxy-5-heptyl-thiophene (TJ191) is a selective anti-cancer small molecule that targets low TβRIII-expressing malignant T-cell leukemia/lymphoma cells. Oncotarget. 2017 Dec 15;9(5):6259-6269. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9157 mL	19.5787 mL	39.1573 mL	97.8933 mL
	5 mM	0.7831 mL	3.9157 mL	7.8315 mL	19.5787 mL
	10 mM	0.3916 mL	1.9579 mL	3.9157 mL	9.7893 mL
	15 mM	0.2610 mL	1.3052 mL	2.6105 mL	6.5262 mL
	20 mM	0.1958 mL	0.9789 mL	1.9579 mL	4.8947 mL
	25 mM	0.1566 mL	0.7831 mL	1.5663 mL	3.9157 mL
	30 mM	0.1305 mL	0.6526 mL	1.3052 mL	3.2631 mL
	40 mM	0.0979 mL	0.4895 mL	0.9789 mL	2.4473 mL
	50 mM	0.0783 mL	0.3916 mL	0.7831 mL	1.9579 mL
	60 mM	0.0653 mL	0.3263 mL	0.6526 mL	1.6316 mL
	80 mM	0.0489 mL	0.2447 mL	0.4895 mL	1.2237 mL
	100 mM	0.0392 mL	0.1958 mL	0.3916 mL	0.9789 mL

TJ191 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TJ1911522415-97-9TJ 191TJ-191ApoptosisCEMJURKATMOLT-3MOLT-4SUP-T1MT-2C8166HSB-2apoptosiscancerPMBCInhibitorinhibitorinhibit

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TJ191

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