Triamterene

Name: Triamterene
Brand: MedChemExpress
SKU: HY-B0575
Rating: 5.0 (5 reviews)

Cat. No.: HY-B0575 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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Triamterene blocks epithelial Na⁺ channel (ENaC) in a voltage-dependent manner, which used as a mild diuretic. Triamterene is an inhibitor of the TGR5 receptor.

For research use only. We do not sell to patients.

Triamterene Chemical Structure

CAS No. : 396-01-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
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5 g		Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Triamterene:

Triamterene-d₅ Get quote
Triamterene (Standard) Get quote

4 Publications Citing Use of MCE Triamterene

: Triamterene purchased from MedChemExpress. Usage Cited in: Immunobiology. 2023 May,152388.; Triamterene (20 μM; 2 h) abolishes the TCDCA-inhibited phosphorylation of AKT, IκBα and NFκB in C6 cells.

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Na_v1.1 Na_v1.2 Na_v1.3 Na_v1.4 Na_v1.5 Na_v1.6 Na_v1.7 Na_v1.8

Biological Activity
Purity & Documentation
References
Customer Review

Description

Triamterene blocks epithelial Na⁺ channel (ENaC) in a voltage-dependent manner, which used as a mild diuretic. Triamterene is an inhibitor of the TGR5 receptor^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	210 μM Compound: 11	Inhibitory activity against CDK4 using recombinant human cyclin D2-CDK4 complex expressed in sf9 cells Inhibitory activity against CDK4 using recombinant human cyclin D2-CDK4 complex expressed in sf9 cells	[PMID: 11741479]

In Vitro

Triamterene shows cytotoxicity against HCT116 and CT26 cells, with IC₅₀ values of 31.30 and 24.45 μM^[5].
Triamterene (100 and 200 µM, 2 h) increases lysosomal rupture, decreases integrity of lysosomes, and activates lysophagy in HepG2 cells^[6].
Triamterene (10-100 µM) inhibits the delayed rectifier potassium current in guinea pig ventricular myocytes^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[6]

Cell Line:	HepG2 cells
Concentration:	100 and 200 µM
Incubation Time:	2 h
Result:	Induced Gal3-puncta formation. Induced the translocation of TFEB to the nucleus from the cytosol.

In Vivo

Triamterene (10-40 mg/kg/day, p.o., 5 days) shows anticonvulsant effects in seizure models of mice induced by intravenous Pentylenetetrazole (PTZ) (0.5%, 1 mL/min), intraperitoneal PTZ (85 mg/kg) and maximal electroshock seizure (MES)^[3].
Triamterene (25 mg/kg) reduces urinary magnesium excretion in conscious saline-loaded rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

253.26

Formula

C₁₂H₁₁N₇

CAS No.

396-01-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=NC(N)=C2N=C(C3=CC=CC=C3)C(N)=NC2=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (19.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.1 mg/mL (0.39 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9485 mL	19.7426 mL	39.4851 mL
5 mM	0.7897 mL	3.9485 mL	7.8970 mL
10 mM	0.3949 mL	1.9743 mL	3.9485 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (249 KB) HNMR (267 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Busch, A.E., et al., Blockade of epithelial Na+ channels by triamterenes - underlying mechanisms and molecular basis. Pflugers Arch, 1996. 432(5): p. 760-6. [Content Brief]

[2]. Gilfrich, H.J., et al., Pharmacokinetics of triamterene after i.v. administration to man: determination of bioavailability. Eur J Clin Pharmacol, 1983. 25(2): p. 237-41. [Content Brief]

[3]. Shafaroodi H, et al. A role for ATP-sensitive potassium channels in the anticonvulsant effects of triamterene in mice. Epilepsy Res. 2016 Mar;121:8-13. [Content Brief]

[4]. Devane J, et al. The effects of amiloride and triamterene on urinary magnesium excretion in conscious saline-loaded rats. Br J Pharmacol. 1981 Feb;72(2):285-9. [Content Brief]

[5]. Moghadam NH, et al. In vitro cytotoxicity and DNA/HSA interaction study of triamterene using molecular modelling and multi-spectroscopic methods. J Biomol Struct Dyn. 2019 Jun;37(9):2242-2253. [Content Brief]

[6]. Park NY, et al. Triamterene induces autophagic degradation of lysosome by exacerbating lysosomal integrity. Arch Pharm Res. 2021 Jun;44(6):621-631. [Content Brief]

[7]. Daleau P, et al. Triamterene inhibits the delayed rectifier potassium current (IK) in guinea pig ventricular myocytes. Circ Res. 1994 Jun;74(6):1114-20. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9485 mL	19.7426 mL	39.4851 mL	98.7128 mL
	5 mM	0.7897 mL	3.9485 mL	7.8970 mL	19.7426 mL
	10 mM	0.3949 mL	1.9743 mL	3.9485 mL	9.8713 mL
	15 mM	0.2632 mL	1.3162 mL	2.6323 mL	6.5809 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Triamterene Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triamterene396-01-0Sodium ChannelG protein-coupled Bile Acid Receptor 1Na channelsNa+ channelsG-protein coupled receptor 19GPCR19TGR5GPBAR1anticonvulsantseizureanticancerHCT116CT26Inhibitorinhibitorinhibit

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Triamterene

Customer Review

Other Forms of Triamterene:

Triamterene Related Antibodies

4 Publications Citing Use of MCE Triamterene

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•Related Screening Libraries:

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View All Sodium Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Triamterene Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Triamterene Related Classifications

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