2. Epigenetics
  3. Epigenetic Reader Domain
  4. UMB298

UMB298 is a potent and selective CBP/P300 bromodomain inhibitor.

For research use only. We do not sell to patients.

UMB298 Chemical Structure

UMB298 Chemical Structure

CAS No. : 2266569-73-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 306 In-stock
Solution
10 mM * 1 mL in DMSO USD 306 In-stock
Solid
5 mg USD 290 In-stock
10 mg USD 465 In-stock
25 mg USD 800 In-stock
50 mg USD 1120 In-stock
100 mg USD 1540 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

UMB298 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

UMB298 is a potent and selective CBP/P300 bromodomain inhibitor[1].

IC50 & Target[1]

BRD4

5193 nM (IC50)

In Vitro

UMB298 (0.01~10 μM; 50 days; MOLM13 and MM cells) inhibits cells growth[1].
UMB298 (1~10 μM; 2 hours; MOLM13 cells) reduces the H3K27ac level similar to CBP30 and causes MYC depletion as a signature of CBP inhibition in acute myeloid leukemia[1].
UMB298 (3 μM; 2hours; MOLM13 cells) down-regulates MYC expression[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

UMB298 Related Antibodies

Cell Viability Assay[1]

Cell Line: MOLM13 and MM cells
Concentration: 0.01~10 μM
Incubation Time: 50 days
Result: Inhibited cells growth.

Western Blot Analysis[1]

Cell Line: MOLM13 cells
Concentration: 1~10 μM
Incubation Time: 2 hours
Result: Reduced the H3K27ac level similar to CBP30 and caused MYC depletion as a signature of CBP inhibition in acute myeloid leukemia.

RT-PCR[1]

Cell Line: MOLM13 cells
Concentration: 3 μM
Incubation Time: 2 hours
Result: Down-regulated MYC expression.
Molecular Weight

479.01

Formula

C27H31ClN4O2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CC1=C(C2=CC3=NC(CCC4=CC=C(OC)C(Cl)=C4)=C(NC5CCCCC5)N3C=C2)C(C)=NO1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (52.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0876 mL 10.4382 mL 20.8764 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.22 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0876 mL 10.4382 mL 20.8764 mL 52.1910 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL 10.4382 mL
10 mM 0.2088 mL 1.0438 mL 2.0876 mL 5.2191 mL
15 mM 0.1392 mL 0.6959 mL 1.3918 mL 3.4794 mL
20 mM 0.1044 mL 0.5219 mL 1.0438 mL 2.6095 mL
25 mM 0.0835 mL 0.4175 mL 0.8351 mL 2.0876 mL
30 mM 0.0696 mL 0.3479 mL 0.6959 mL 1.7397 mL
40 mM 0.0522 mL 0.2610 mL 0.5219 mL 1.3048 mL
50 mM 0.0418 mL 0.2088 mL 0.4175 mL 1.0438 mL
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UMB298 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

UMB2982266569-73-5UMB 298UMB-298Epigenetic Reader DomainpotentselectiveMOLM13MMbromodomainCBP30Inhibitorinhibitorinhibit

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