Verubulin (Synonyms: MPC 6827)

Name: Verubulin
Brand: MedChemExpress
SKU: HY-14907
Rating: 4.5 (1 reviews)

Cat. No.: HY-14907 Purity: 99.34%: FAQs
Data Sheet SDS COA Handling Instructions

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Verubulin (MPC-6827) is a microtubule-disrupting agent with potent and broad-spectrum in vitro and in vivo cytotoxic activities, and acts as a promising candidate for the treatment of multiple cancer types.

For research use only. We do not sell to patients.

Verubulin Chemical Structure

CAS No. : 827031-83-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 88	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 88	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 475	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 760	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Verubulin:

Verubulin hydrochloride Get quote

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Biological Activity
Purity & Documentation
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Description

IC₅₀ & Target

Microtubule^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	0.7 nM Compound: Verubulin	Antiproliferative activity against human A-375 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTS assay Antiproliferative activity against human A-375 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTS assay	[PMID: 34406768]
A375/TxR	IC₅₀	0.7 nM Compound: Verubulin	Antiproliferative activity against human A375/TxR cells assessed as inhibition of cell proliferation measured after 72 hrs by MTS assay Antiproliferative activity against human A375/TxR cells assessed as inhibition of cell proliferation measured after 72 hrs by MTS assay	[PMID: 34406768]
A-431	IC₅₀	0.9 nM Compound: 1	Cytotoxicity against human A-431 cells assessed as reduction in cell viability by Sulforhodamine B assay Cytotoxicity against human A-431 cells assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]
A549	IC₅₀	0.003 μM Compound: EP128495	Growth inhibition of human A549 cells after 48 hrs by MTT assay Growth inhibition of human A549 cells after 48 hrs by MTT assay	[PMID: 29509413]
A549	IC₅₀	0.004 μM Compound: MPC-6827	Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay	[PMID: 29673981]
A549	IC₅₀	0.005 μM Compound: MPC6827	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 30093295]
A549/CDDP	IC₅₀	0.009 μM Compound: EP128495	Growth inhibition of human A549/CDDP cells after 48 hrs by MTT assay Growth inhibition of human A549/CDDP cells after 48 hrs by MTT assay	[PMID: 29509413]
BJ	IC₅₀	0.015 μM Compound: EP128495	Antiproliferative activity against human BJ cells after 48 hrs by MTT assay Antiproliferative activity against human BJ cells after 48 hrs by MTT assay	[PMID: 29509413]
HCT-116	EC₅₀	0.003 μM Compound: 6h, EP128495,MPC-6827, azixa	Induction of apoptosis in human HCT116 cells assessed as caspase activation after 24 hrs by HTS assay Induction of apoptosis in human HCT116 cells assessed as caspase activation after 24 hrs by HTS assay	[PMID: 19296653]
HCT-116	IC₅₀	0.004 μM Compound: EP128495	Growth inhibition of human HCT116 cells after 48 hrs by MTT assay Growth inhibition of human HCT116 cells after 48 hrs by MTT assay	[PMID: 29509413]
HCT-116	IC₅₀	0.005 μM Compound: MPC-6827	Antiproliferative activity against human HCT116 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells incubated for 48 hrs by MTT assay	[PMID: 29673981]
HCT-116	GI₅₀	0.006 μM Compound: 6h, EP128495,MPC-6827, azixa	Growth inhibition of human HCT116 cells after 48 hrs by celltiter assay Growth inhibition of human HCT116 cells after 48 hrs by celltiter assay	[PMID: 19296653]
HCT-116	IC₅₀	0.009 μM Compound: MPC6827	Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 30093295]
HCT-116	IC₅₀	4.3 nM Compound: 5; MPC-6827	Antiproliferative activity against human HCT116 cells after 24 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 27213819]
HeLa	IC₅₀	0.005 μM Compound: EP128495	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 29509413]
HeLa	IC₅₀	0.006 μM Compound: MPC-6827	Antiproliferative activity against human HeLa cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HeLa cells incubated for 48 hrs by MTT assay	[PMID: 29673981]
HeLa	IC₅₀	0.007 μM Compound: MPC6827	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 30093295]
HeLa	IC₅₀	1.5 nM Compound: 1	Cytotoxicity against human HeLa cells over expressing WT-beta3 assessed as reduction in cell viability by Sulforhodamine B assay Cytotoxicity against human HeLa cells over expressing WT-beta3 assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]
HeLa	IC₅₀	2 nM Compound: 1	Cytotoxicity against human HeLa cells assessed as reduction in cell viability by Sulforhodamine B assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]
HepG2	IC₅₀	0.005 μM Compound: MPC-6827	Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay	[PMID: 29673981]
HepG2	IC₅₀	0.005 μM Compound: EP128495	Growth inhibition of human HepG2 cells after 48 hrs by MTT assay Growth inhibition of human HepG2 cells after 48 hrs by MTT assay	[PMID: 29509413]
HepG2	IC₅₀	0.006 μM Compound: MPC6827	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 30093295]
HepG2	IC₅₀	4.6 nM Compound: 5; MPC-6827	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 27213819]
L02	IC₅₀	0.013 μM Compound: EP128495	Antiproliferative activity against human LO2 cells after 48 hrs by MTT assay Antiproliferative activity against human LO2 cells after 48 hrs by MTT assay	[PMID: 29509413]
LoVo	IC₅₀	0.008 μM Compound: EP128495	Growth inhibition of human LoVo cells after 48 hrs by MTT assay Growth inhibition of human LoVo cells after 48 hrs by MTT assay	[PMID: 29509413]
M14	IC₅₀	1 nM Compound: Verubulin	Antiproliferative activity against human M14 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTS assay Antiproliferative activity against human M14 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTS assay	[PMID: 34406768]
MCF7	IC₅₀	0.011 μM Compound: EP128495	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 29509413]
MDA-MB-231	IC₅₀	0.008 μM Compound: MPC-6827	Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay	[PMID: 29673981]
MDA-MB-231	IC₅₀	0.008 μM Compound: EP128495	Growth inhibition of human MDA-MB-231 cells after 48 hrs by MTT assay Growth inhibition of human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 29509413]
MDA-MB-435	IC₅₀	1.7 nM Compound: 1	Antiproliferative activity against human MDA-MB-435 cells assessed as reduction in cell viability by Sulforhodamine B assay Antiproliferative activity against human MDA-MB-435 cells assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]
MGC-803	IC₅₀	0.008 μM Compound: EP128495	Antiproliferative activity against human MGC803 cells after 48 hrs by MTT assay Antiproliferative activity against human MGC803 cells after 48 hrs by MTT assay	[PMID: 29509413]
NCI-H1299	EC₅₀	0.006 μM Compound: 1e	Induction of apoptosis in human H1299 cells assessed as caspase activation after 48 hrs by HTS assay Induction of apoptosis in human H1299 cells assessed as caspase activation after 48 hrs by HTS assay	[PMID: 19464890]
RKO	IC₅₀	0.003 μM Compound: EP128495	Growth inhibition of human RKO cells after 48 hrs by MTT assay Growth inhibition of human RKO cells after 48 hrs by MTT assay	[PMID: 29509413]
RKO	IC₅₀	0.005 μM Compound: MPC6827	Antiproliferative activity against human RKO cells after 48 hrs by MTT assay Antiproliferative activity against human RKO cells after 48 hrs by MTT assay	[PMID: 30093295]
SK-OV-3	IC₅₀	2.2 nM Compound: 1	Cytotoxicity against human SK-OV-3 cells assessed as reduction in cell viability by Sulforhodamine B assay Cytotoxicity against human SK-OV-3 cells assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]
SK-OV-3	IC₅₀	2.6 nM Compound: 1	Cytotoxicity against human SK-OV-3 cells over expressing MDR1-6/6 assessed as reduction in cell viability by Sulforhodamine B assay Cytotoxicity against human SK-OV-3 cells over expressing MDR1-6/6 assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]
SK-OV-3	IC₅₀	3.2 nM Compound: 5; MPC-6827	Antiproliferative activity against human SKOV3 cells after 24 hrs by MTT assay Antiproliferative activity against human SKOV3 cells after 24 hrs by MTT assay	[PMID: 27213819]
SNU-398	EC₅₀	0.002 μM Compound: 6h, EP128495,MPC-6827, azixa	Induction of apoptosis in human SNU398 cells assessed as caspase activation after 24 hrs by HTS assay Induction of apoptosis in human SNU398 cells assessed as caspase activation after 24 hrs by HTS assay	[PMID: 19296653]
SNU-398	GI₅₀	0.003 μM Compound: 6h, EP128495,MPC-6827, azixa	Growth inhibition of human SNU398 cells after 48 hrs by celltiter assay Growth inhibition of human SNU398 cells after 48 hrs by celltiter assay	[PMID: 19296653]
T47D	EC₅₀	0.002 μM Compound: 6h, EP128495,MPC-6827, azixa	Induction of apoptosis in human T47D cells assessed as caspase activation after 24 hrs by HTS assay Induction of apoptosis in human T47D cells assessed as caspase activation after 24 hrs by HTS assay	[PMID: 19296653]
T47D	EC₅₀	0.002 μM Compound: 1e	Induction of apoptosis in human T47D cells assessed as caspase activation after 48 hrs by HTS assay Induction of apoptosis in human T47D cells assessed as caspase activation after 48 hrs by HTS assay	[PMID: 19464890]
T47D	GI₅₀	0.006 μM Compound: 6h, EP128495,MPC-6827, azixa	Growth inhibition of human T47D cells after 48 hrs by celltiter assay Growth inhibition of human T47D cells after 48 hrs by celltiter assay	[PMID: 19296653]
U-251	IC₅₀	2.8 nM Compound: 1	Cytotoxicity against human U-251 cells assessed as reduction in cell viability by Sulforhodamine B assay Cytotoxicity against human U-251 cells assessed as reduction in cell viability by Sulforhodamine B assay	[PMID: 33647840]

Clinical Trial

Molecular Weight

279.34

Formula

C₁₇H₁₇N₃O

CAS No.

827031-83-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=NC(N(C2=CC=C(OC)C=C2)C)=C3C=CC=CC3=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (357.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5799 mL	17.8993 mL	35.7987 mL
5 mM	0.7160 mL	3.5799 mL	7.1597 mL
10 mM	0.3580 mL	1.7899 mL	3.5799 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.34%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (251 KB) HNMR (189 KB) LCMS (119 KB)

Handling Instructions (2659 KB)

References

[1]. Kasibhatla S, et al. MPC-6827: a small-molecule inhibitor of microtubule formation that is not a substrate for multidrug resistance pumps. Cancer Res. 2007 Jun 15;67(12):5865-71. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5799 mL	17.8993 mL	35.7987 mL	89.4967 mL
	5 mM	0.7160 mL	3.5799 mL	7.1597 mL	17.8993 mL
	10 mM	0.3580 mL	1.7899 mL	3.5799 mL	8.9497 mL
	15 mM	0.2387 mL	1.1933 mL	2.3866 mL	5.9664 mL
	20 mM	0.1790 mL	0.8950 mL	1.7899 mL	4.4748 mL
	25 mM	0.1432 mL	0.7160 mL	1.4319 mL	3.5799 mL
	30 mM	0.1193 mL	0.5966 mL	1.1933 mL	2.9832 mL
	40 mM	0.0895 mL	0.4475 mL	0.8950 mL	2.2374 mL
	50 mM	0.0716 mL	0.3580 mL	0.7160 mL	1.7899 mL
	60 mM	0.0597 mL	0.2983 mL	0.5966 mL	1.4916 mL
	80 mM	0.0447 mL	0.2237 mL	0.4475 mL	1.1187 mL
	100 mM	0.0358 mL	0.1790 mL	0.3580 mL	0.8950 mL

Verubulin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Verubulin827031-83-4MPC 6827MPC6827MPC-6827Microtubule/TubulinApoptosisInhibitorinhibitorinhibit

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Verubulin (Synonyms: MPC 6827)

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Complete Stock Solution Preparation Table

Verubulin Related Classifications

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