YM-1

Name: YM-1
Brand: MedChemExpress
SKU: HY-18399
Rating: 4.5 (1 reviews)

Cat. No.: HY-18399 Purity: 99.25%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

YM-1 is a stable and soluble MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins.

For research use only. We do not sell to patients.

YM-1 Chemical Structure

CAS No. : 409086-68-6

Size	Price	Stock	Quantity
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1350	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of YM-1:

HSP70-IN-4 Get quote

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

YM-1 is a stable and soluble MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	EC₅₀	5.2 μM Compound: YM-01	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 24312699]
MDA-MB-231	EC₅₀	2 μM Compound: YM-01	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 24312699]
MEF	EC₅₀	> 50 μM Compound: YM-01	Cytotoxicity against C57BL/6 mouse MEF assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against C57BL/6 mouse MEF assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 24312699]

In Vitro

YM-1 promotes Hsp70-dependent steps in nNOS maturation and partially blocks formation of the ATP-bound form^[1].
YM-1 (0-200 μM) activates the binding of Hsp70 to its unfolded substrate^[1].
YM-1 (0.001-1000 μM) converts Hsp70 to its tight-affinity conformation and shows binding efficacy to Hsp70 with an IC₅₀ value of 8.2 μM^[1].
YM-1 (0, 0.1, 0.5 and 1 μM; 24 hours) promotes nNOS ubiquitination^[1].
YM-1 (5 and 10 μM; 24 and 48 hours) induces cell death of HeLa cells and growth arrest of hTERT-RPE1 cells^[2].
YM-1 (10 μM; 48 hours) up-regulates p53 and p21 proteins and down-regulates FoxM1 and survivin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	HeLa and hTERT-RPE1 cell lines
Concentration:	10 μM
Incubation Time:	48 hoursI
Result:	Increased the level of p53 and p21 and decreased the level of FoxM1 and survivin.

In Vivo

YM-1 (1 mM; oral administration, for 7 days) rescues polyQ toxicity in Drosophila by activating Hsp70^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	UAS-hAR52Q flies with polyQ AR-induced dihydrotestosterone (DHT) phenotype^[1]
Dosage:	1 mM
Administration:	Oral administration; 1 mM, for 7 days
Result:	Weakened the DHT-dependent eye degeneration phenotype and rescued DHT-dependent pupal toxicity of the polyQ AR.

Molecular Weight

417.98

Formula

C₂₀H₂₀ClN₃OS₂

CAS No.

409086-68-6

Appearance

Solid

Color

Brown to reddish brown

SMILES

CN(C1=CC=CC=C1S/2)C2=C3C(N(CC)/C(S\3)=C/C4=[N+](C)C=CC=C4)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (119.62 mM; Need ultrasonic)

DMSO : 30 mg/mL (71.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3925 mL	11.9623 mL	23.9246 mL
5 mM	0.4785 mL	2.3925 mL	4.7849 mL
10 mM	0.2392 mL	1.1962 mL	2.3925 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.25%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (256 KB) HNMR (442 KB) LCMS (101 KB)

Handling Instructions (2659 KB)

References

[1]. Wang AM, et al. Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Nat Chem Biol. 2013 Feb;9(2):112-8. [Content Brief]

[2]. Khondoker Md Zulfiker Rahman, et al. Effect of an Inhibitor of HSP70, YM-1, on Hikeshi Knockout Cells. Thermal Medicine. 2017, 33(4):129-134.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.3925 mL	11.9623 mL	23.9246 mL	59.8115 mL
	5 mM	0.4785 mL	2.3925 mL	4.7849 mL	11.9623 mL
	10 mM	0.2392 mL	1.1962 mL	2.3925 mL	5.9811 mL
	15 mM	0.1595 mL	0.7975 mL	1.5950 mL	3.9874 mL
	20 mM	0.1196 mL	0.5981 mL	1.1962 mL	2.9906 mL
	25 mM	0.0957 mL	0.4785 mL	0.9570 mL	2.3925 mL
	30 mM	0.0797 mL	0.3987 mL	0.7975 mL	1.9937 mL
	40 mM	0.0598 mL	0.2991 mL	0.5981 mL	1.4953 mL
	50 mM	0.0478 mL	0.2392 mL	0.4785 mL	1.1962 mL
	60 mM	0.0399 mL	0.1994 mL	0.3987 mL	0.9969 mL
H₂O	80 mM	0.0299 mL	0.1495 mL	0.2991 mL	0.7476 mL
H₂O	100 mM	0.0239 mL	0.1196 mL	0.2392 mL	0.5981 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

YM-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

YM-1409086-68-6YM1YM 1HSPNO SynthaseHeat shock proteinsNitric oxide synthasesNOSMKT-077nNOSubiquitinationHeLahTERT-RPE1polyQDrosophilaInhibitorinhibitorinhibit

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YM-1

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Other Forms of YM-1:

YM-1 Related Antibodies

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YM-1 Related Antibodies

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Complete Stock Solution Preparation Table

YM-1 Related Classifications

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