2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease Immunology/Inflammation
  3. HSP NO Synthase
  4. YM-1

YM-1 is a stable and soluble MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins.

For research use only. We do not sell to patients.

YM-1 Chemical Structure

YM-1 Chemical Structure

CAS No. : 409086-68-6

Size Price Stock Quantity
5 mg USD 150 In-stock
10 mg USD 250 In-stock
25 mg USD 550 In-stock
50 mg USD 850 In-stock
100 mg USD 1350 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of YM-1:

YM-1 Related Antibodies

Top Publications Citing Use of Products

View All HSP Isoform Specific Products:

View All Isoforms
HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

View All NO Synthase Isoform Specific Products:

View All Isoforms
nNOS iNOS eNOS

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

YM-1 is a stable and soluble MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins[1][2].
In Vitro

YM-1 promotes Hsp70-dependent steps in nNOS maturation and partially blocks formation of the ATP-bound form[1].
YM-1 (0-200 μM) activates the binding of Hsp70 to its unfolded substrate[1].
YM-1 (0.001-1000 μM) converts Hsp70 to its tight-affinity conformation and shows binding efficacy to Hsp70 with an IC50 value of 8.2 μM[1].
YM-1 (0, 0.1, 0.5 and 1 μM; 24 hours) promotes nNOS ubiquitination[1].
YM-1 (5 and 10 μM; 24 and 48 hours) induces cell death of HeLa cells and growth arrest of hTERT-RPE1 cells[2].
YM-1 (10 μM; 48 hours) up-regulates p53 and p21 proteins and down-regulates FoxM1 and survivin[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

YM-1 Related Antibodies

Western Blot Analysis[2]

Cell Line: HeLa and hTERT-RPE1 cell lines
Concentration: 10 μM
Incubation Time: 48 hoursI
Result: Increased the level of p53 and p21 and decreased the level of FoxM1 and survivin.
In Vivo

YM-1 (1 mM; oral administration, for 7 days) rescues polyQ toxicity in Drosophila by activating Hsp70[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: UAS-hAR52Q flies with polyQ AR-induced dihydrotestosterone (DHT) phenotype[1]
Dosage: 1 mM
Administration: Oral administration; 1 mM, for 7 days
Result: Weakened the DHT-dependent eye degeneration phenotype and rescued DHT-dependent pupal toxicity of the polyQ AR.
Molecular Weight

417.98

Formula

C20H20ClN3OS2
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

CN(C1=CC=CC=C1S/2)C2=C3C(N(CC)/C(S\3)=C/C4=[N+](C)C=CC=C4)=O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (119.62 mM; Need ultrasonic)

DMSO : 30 mg/mL (71.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3925 mL 11.9623 mL 23.9246 mL
5 mM 0.4785 mL 2.3925 mL 4.7849 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.25%

COA (256 KB) HNMR (442 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.3925 mL 11.9623 mL 23.9246 mL 59.8115 mL
5 mM 0.4785 mL 2.3925 mL 4.7849 mL 11.9623 mL
10 mM 0.2392 mL 1.1962 mL 2.3925 mL 5.9811 mL
15 mM 0.1595 mL 0.7975 mL 1.5950 mL 3.9874 mL
20 mM 0.1196 mL 0.5981 mL 1.1962 mL 2.9906 mL
25 mM 0.0957 mL 0.4785 mL 0.9570 mL 2.3925 mL
30 mM 0.0797 mL 0.3987 mL 0.7975 mL 1.9937 mL
40 mM 0.0598 mL 0.2991 mL 0.5981 mL 1.4953 mL
50 mM 0.0478 mL 0.2392 mL 0.4785 mL 1.1962 mL
60 mM 0.0399 mL 0.1994 mL 0.3987 mL 0.9969 mL
H2O 80 mM 0.0299 mL 0.1495 mL 0.2991 mL 0.7476 mL
100 mM 0.0239 mL 0.1196 mL 0.2392 mL 0.5981 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

YM-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

YM-1409086-68-6YM1YM 1HSPNO SynthaseHeat shock proteinsNitric oxide synthasesNOSMKT-077nNOSubiquitinationHeLahTERT-RPE1polyQDrosophilaInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
YM-1
Cat. No.:
HY-18399
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.