YW1128

Name: YW1128
Brand: MedChemExpress
SKU: HY-133180
Rating: 4.5 (1 reviews)

Cat. No.: HY-133180 Purity: 99.38%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

YW1128 (compound 3a) is a potent Wnt/β-Catenin inhibitor. YW1128 induces the proteasome degradation of β-catenin and subsequent inhibits the Wnt/β-catenin signaling in cells. YW1128 significantly decreases hepatic lipid accumulation. YW1128 improves glucose tolerance of high fat diet-fed mice without noticeable toxicity. YW1128 down regulates the genes involved in the glucose and fatty acid anabolism.

For research use only. We do not sell to patients.

YW1128 Chemical Structure

CAS No. : 2131223-64-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 225	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 225	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 205	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 659	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1055	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1688	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	20 nM Compound: 3a	Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring siRNA after 24 hrs by luciferase reporter gene assay Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring siRNA after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
HEK293	IC₅₀	34 nM Compound: 3a	Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring GSK3B siRNA after 24 hrs by luciferase reporter gene assay Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring GSK3B siRNA after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
HEK293	IC₅₀	4.1 nM Compound: 3a	Inhibition of Wnt3a activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by Wnt3a addition and measured after 24 hrs by luciferase reporter gene assay Inhibition of Wnt3a activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by Wnt3a addition and measured after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
HEK293	IC₅₀	6 nM Compound: 3a	Inhibition of LiCl activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by LiCl addition and measured after 24 hrs by luciferase reporter gene assay Inhibition of LiCl activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by LiCl addition and measured after 24 hrs by luciferase reporter gene assay	[PMID: 30605343]
K562	IC₅₀	> 50 μM Compound: 1	Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 35777269]

Molecular Weight

343.38

Formula

C₂₀H₁₇N₅O

CAS No.

2131223-64-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C(C)N(C2=CC=CC=C2C)N=N1)NC3=NC4=CC=CC=C4C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (291.22 mM; ultrasonic and warming and heat to 160°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9122 mL	14.5611 mL	29.1223 mL
5 mM	0.5824 mL	2.9122 mL	5.8245 mL
10 mM	0.2912 mL	1.4561 mL	2.9122 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (7.28 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.38%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (249 KB) HNMR (137 KB) RP-HPLC (168 KB) LCMS (232 KB)

Handling Instructions (2659 KB)

References

[1]. Obianom ON, et al. Triazole-Based Inhibitors of the Wnt/β-Catenin Signaling Pathway Improve Glucose and Lipid Metabolisms in Diet-Induced Obese Mice. J Med Chem. 2019 Jan 24;62(2):727-741. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9122 mL	14.5611 mL	29.1223 mL	72.8056 mL
	5 mM	0.5824 mL	2.9122 mL	5.8245 mL	14.5611 mL
	10 mM	0.2912 mL	1.4561 mL	2.9122 mL	7.2806 mL
	15 mM	0.1941 mL	0.9707 mL	1.9415 mL	4.8537 mL
	20 mM	0.1456 mL	0.7281 mL	1.4561 mL	3.6403 mL
	25 mM	0.1165 mL	0.5824 mL	1.1649 mL	2.9122 mL
	30 mM	0.0971 mL	0.4854 mL	0.9707 mL	2.4269 mL
	40 mM	0.0728 mL	0.3640 mL	0.7281 mL	1.8201 mL
	50 mM	0.0582 mL	0.2912 mL	0.5824 mL	1.4561 mL
	60 mM	0.0485 mL	0.2427 mL	0.4854 mL	1.2134 mL
	80 mM	0.0364 mL	0.1820 mL	0.3640 mL	0.9101 mL
	100 mM	0.0291 mL	0.1456 mL	0.2912 mL	0.7281 mL

YW1128 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

YW11282131223-64-6YW 1128YW-1128Wntβ-cateninBeta cateninHEK293 cellsHepG2SW480 cellslow toxicityaccumulationglucosefatty acid anabolismInhibitorinhibitorinhibit

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YW1128

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Complete Stock Solution Preparation Table

YW1128 Related Classifications

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