ZM223 hydrochloride

Name: ZM223 hydrochloride
Brand: MedChemExpress
SKU: HY-101790A
Rating: 4.5 (1 reviews)

Cat. No.: HY-101790A Purity: 99.21%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ZM223 hydrochloride is an orally active, potent non-covalent NEDD8 activating enzyme (NAE) inhibitor with excellent anticancer activity.

For research use only. We do not sell to patients.

ZM223 hydrochloride Chemical Structure

CAS No. : 2438679-27-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 285	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 285	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 680	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1500	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of ZM223 hydrochloride:

ZM223 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

ZM223 hydrochloride is an orally active, potent non-covalent NEDD8 activating enzyme (NAE) inhibitor with excellent anticancer activity^[1].

IC₅₀ & Target

NEDD8 activating enzyme (NAE)^[1]

In Vitro

ZM223 hydrochloride (0.1-1 μM; 4 hours) inhibits both HCT-116 and U-2OS cancer cells with IC₅₀s of 100 and 122 nM, respectively^[1].
ZM223 hydrochloride (0.1-1 μM; 4 hours) causes a dose-response decrease in the level of NEDD8 and accumulation of the UBC12 protein, indicating the decrease of the subsequent NEDD8-UBC12 complex^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HCT116 colon cancer cells and U-2OS osteosarcoma cells
Concentration:	0.1 μM, 1 μM
Incubation Time:	4 hours
Result:	Inhibited both HCT-116 and U-2OS cancer cells.

Western Blot Analysis^[1]

Cell Line:	HCT116 colon cancer cells
Concentration:	0.1 μM, 1 μM
Incubation Time:	4 hours
Result:	Caused a decrease in the level of NEDD8 and an increase in the downstream UBC12 protein.

Molecular Weight

538.99

Formula

C₂₃H₁₈ClF₃N₄O₂S₂

CAS No.

2438679-27-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(C(F)(F)F)C=C1)NC2=NC3=CC=C(NC(CSC4=CC=C(N)C=C4)=O)C=C3S2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 150 mg/mL (278.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8553 mL	9.2766 mL	18.5532 mL
5 mM	0.3711 mL	1.8553 mL	3.7106 mL
10 mM	0.1855 mL	0.9277 mL	1.8553 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 7.5 mg/mL (13.91 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 7.5 mg/mL (13.91 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 7.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.21%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (244 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Ma H, et al. Discovery of benzothiazole derivatives as novel non-sulfamide NEDD8 activating enzyme inhibitors by target-based virtual screening. Eur J Med Chem. 2017 Jun 16;133:174-183. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8553 mL	9.2766 mL	18.5532 mL	46.3831 mL
	5 mM	0.3711 mL	1.8553 mL	3.7106 mL	9.2766 mL
	10 mM	0.1855 mL	0.9277 mL	1.8553 mL	4.6383 mL
	15 mM	0.1237 mL	0.6184 mL	1.2369 mL	3.0922 mL
	20 mM	0.0928 mL	0.4638 mL	0.9277 mL	2.3192 mL
	25 mM	0.0742 mL	0.3711 mL	0.7421 mL	1.8553 mL
	30 mM	0.0618 mL	0.3092 mL	0.6184 mL	1.5461 mL
	40 mM	0.0464 mL	0.2319 mL	0.4638 mL	1.1596 mL
	50 mM	0.0371 mL	0.1855 mL	0.3711 mL	0.9277 mL
	60 mM	0.0309 mL	0.1546 mL	0.3092 mL	0.7731 mL
	80 mM	0.0232 mL	0.1160 mL	0.2319 mL	0.5798 mL
	100 mM	0.0186 mL	0.0928 mL	0.1855 mL	0.4638 mL