α7 nAchR-JAK2-STAT3 agonist 1

Name: α7 nAchR-JAK2-STAT3 agonist 1
Brand: MedChemExpress
SKU: HY-146066
Rating: 4.5 (1 reviews)

Cat. No.: HY-146066 Purity: 99.54%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

α7 nAchR-JAK2-STAT3 agonist 1 is a potent α7 nAchR-JAK2-STAT3 agonist, with an IC₅₀ value of 0.32 μM for nitric oxide (NO). α7 nAchR-JAK2-STAT3 agonist 1 effectively suppresses the expression of iNOS, IL-1β, and IL-6 in murine RAW264.7 macrophages. α7 nAchR-JAK2-STAT3 agonist 1 can inhibit LPS-induced NO release, NF-κB activation and cytokine production. α7 nAchR-JAK2-STAT3 can be used for researching sepsis.

For research use only. We do not sell to patients.

α7 nAchR-JAK2-STAT3 agonist 1 Chemical Structure

CAS No. : 2108714-20-9

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of α7 nAchR-JAK2-STAT3 agonist 1:

(R)-α7 nAchR-JAK2-STAT3 agonist 1 Get quote

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All JAK Isoform Specific Products:

View All Isoforms

JAK1 JAK2 JAK3 Tyk2 JAK

View All STAT Isoform Specific Products:

View All Isoforms

STAT3 STAT5 STAT6 STAT1

View All NO Synthase Isoform Specific Products:

View All Isoforms

nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC₅₀: 0.32 μM (NO)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	0.32 μM Compound: 28	Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated with compound for 2 hrs followed by LPS stimulation for 24 hrs by Griess reagent based assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated with compound for 2 hrs followed by LPS stimulation for 24 hrs by Griess reagent based assay	[PMID: 35576655]

Molecular Weight

426.50

Formula

C₂₅H₃₀O₆

CAS No.

2108714-20-9

Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=C(O[C@@H](CCCCC1=CC=CC=C1)CCC2=CC=C(OC(C)=O)C(OC(C)=O)=C2)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (234.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3447 mL	11.7233 mL	23.4467 mL
5 mM	0.4689 mL	2.3447 mL	4.6893 mL
10 mM	0.2345 mL	1.1723 mL	2.3447 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.86 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (253 KB) HNMR (144 KB) RP-HPLC (169 KB) LCMS (389 KB)

Handling Instructions (2659 KB)

References

[1]. Lin Y, et al. Discovery of diarylheptanoids that activate α7 nAchR-JAK2-STAT3 signaling in macrophages with anti-inflammatory activity in vitro and in vivo. Bioorg Med Chem. 2022;66:116811. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3447 mL	11.7233 mL	23.4467 mL	58.6166 mL
	5 mM	0.4689 mL	2.3447 mL	4.6893 mL	11.7233 mL
	10 mM	0.2345 mL	1.1723 mL	2.3447 mL	5.8617 mL
	15 mM	0.1563 mL	0.7816 mL	1.5631 mL	3.9078 mL
	20 mM	0.1172 mL	0.5862 mL	1.1723 mL	2.9308 mL
	25 mM	0.0938 mL	0.4689 mL	0.9379 mL	2.3447 mL
	30 mM	0.0782 mL	0.3908 mL	0.7816 mL	1.9539 mL
	40 mM	0.0586 mL	0.2931 mL	0.5862 mL	1.4654 mL
	50 mM	0.0469 mL	0.2345 mL	0.4689 mL	1.1723 mL
	60 mM	0.0391 mL	0.1954 mL	0.3908 mL	0.9769 mL
	80 mM	0.0293 mL	0.1465 mL	0.2931 mL	0.7327 mL
	100 mM	0.0234 mL	0.1172 mL	0.2345 mL	0.5862 mL