2. PI3K/Akt/mTOR NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. PI3K Akt Reactive Oxygen Species
  4. 1,3-Dicaffeoylquinic acid

1,3-Dicaffeoylquinic acid  (Synonyms: 1,3-O-Dicaffeoylquinic acid; 1,5-Dicaffeoylquinic acid)

Cat. No.: HY-N1412 Purity: 99.09%
SDS COA Handling Instructions

Molarity Calculator

1,3-Dicaffeoylquinic acid is a caffeoylquinic acid derivative that exhibits antioxidant activity and radical scavenging activity.

For research use only. We do not sell to patients.

1,3-Dicaffeoylquinic acid Chemical Structure

1,3-Dicaffeoylquinic acid Chemical Structure

CAS No. : 19870-46-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 159 In-stock
Solution
10 mM * 1 mL in DMSO USD 159 In-stock
Solid
1 mg USD 66 In-stock
5 mg USD 140 In-stock
10 mg USD 220 In-stock
25 mg USD 450 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of 1,3-Dicaffeoylquinic acid:

1,3-Dicaffeoylquinic acid Related Antibodies

Top Publications Citing Use of Products

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

1,3-Dicaffeoylquinic acid is a caffeoylquinic acid derivative that exhibits antioxidant activity and radical scavenging activity.

IC50 & Target[2]

PI3K

 

Cellular Effect
Cell Line Type Value Description References
BV-2 IC50
22.63 μM
Compound: 6
Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control
Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control
[PMID: 23462643]
MDA-MB-231 IC50
81.85 μM
Compound: 22
Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition by MTT assay
Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition by MTT assay
[PMID: 33650861]
In Vitro

1,3-Dicaffeoylquinic acid shows increased neuronal cell viability against Aβ(42) toxicity in a concentration-dependent manner in neurons. 1,3-Dicaffeoylquinic acid activates both phosphoinositide 3-kinase (PI3K)/Akt and extracellular regulated protein kinase 1/2 (Erk1/2) with stimulating their upstream tyrosine kinase A (Trk A). 1,3-Dicaffeoylquinic acid's anti-apoptotic potential is related to the enhanced inactivating phosphorylation of glycogen synthase kinase 3β (GSK3β) and the modulation of expression of apoptosis-related protein Bcl-2/Bax[2]. 1,3-Dicaffeoylquinic acid (10 μM, 20 μM, 50 μM, and 100 μM) significantly increases cell viablity before OGD/reperfusion, and prevents the depletion of GSH under OGD/reperfusion insult. 1,3-Dicaffeoylquinic acid induces nuclear translocation of Nrf2 in OGD/reperfusion treated astrocytes, and induces increased GCL activity, and the effect is lost in Nrf2 siRNA-transfected cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1,3-Dicaffeoylquinic acid Related Antibodies
In Vivo

1,3-Dicaffeoylquinic acid (32.0 mg/kg, p.o.) and 1-O-ABL are absorbed very quickly in Wistar rats. The maximum plasma concentrations for 1,3-Dicaffeoylquinic acid and 1-O-ABL are 44.5 ± 7.1 and 19.1 ± 6.9 ng/mL, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

516.45

Formula

C25H24O12
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C([C@@]1(OC(/C=C/C2=CC=C(O)C(O)=C2)=O)C[C@@H](OC(/C=C/C3=CC=C(O)C(O)=C3)=O)[C@H](O)[C@H](O)C1)O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (193.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9363 mL 9.6815 mL 19.3630 mL
5 mM 0.3873 mL 1.9363 mL 3.8726 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.09%

SDS (393 KB)

COA (247 KB) HNMR (146 KB) RP-HPLC (249 KB) LCMS (114 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[3]

The whole cellular lysate is prepared using a RIPA Lysis Buffer added to a reaction buffer containing 0.1 M Tris (pH 8.2), 0.15 M KCl, 10 mM ATP, 10 mM l-glutamate, 20 mM MgCl2, and 2 mM EDTA at 37°C for 3 min, and then 5 mM cysteine is added at 37°C for 15 min. The production of glutamylcysteine is immediately quantified for HPLC analysis by O-phthalaldehyde derivatization. GCL activity is presented in units of femtomoles of -GC produced per milligram of protein per minute[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[3]

The viability of astrocytes is measured by the MTT reduction method. Briefly, the cells are rinsed with phosphate-buffered saline, pH 7.2, and incubated with 5 mg/mL MTT reagent for 3 h at 37°C. The medium is removed, and the cells are lysed with 1 mL of dimethyl sulfoxide. The absorbance is measured at 540 nm by a microplate reader[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Six male Wistar rats (200-250 g) are fasted for 12 h with free access to water prior to oral administration of I. britannica extract with an herb dose of 8.0 g/kg (equivalent to 32.0 mg/kg 1,3-Dicaffeoylquinic acid, and 4.01 mg/kg, 1-O-ABL). Blood samples (appr 0.25 mL) are collected from suborbital vein into heparinized tubes at 0, 0.08, 0.16, 0.33, 0.67, 1, 1.5, 2, 4, 6, 9 and 12 h after dosing, and then immediately centrifuged at 3500 rpm for 10 min. Harvested plasma samples are stored at -60°C until analysis. The plasma concentrations of 1,3-Dicaffeoylquinic acid and 1-O-ABL are calculated from the calibration curves obtained daily[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9363 mL 9.6815 mL 19.3630 mL 48.4074 mL
5 mM 0.3873 mL 1.9363 mL 3.8726 mL 9.6815 mL
10 mM 0.1936 mL 0.9681 mL 1.9363 mL 4.8407 mL
15 mM 0.1291 mL 0.6454 mL 1.2909 mL 3.2272 mL
20 mM 0.0968 mL 0.4841 mL 0.9681 mL 2.4204 mL
25 mM 0.0775 mL 0.3873 mL 0.7745 mL 1.9363 mL
30 mM 0.0645 mL 0.3227 mL 0.6454 mL 1.6136 mL
40 mM 0.0484 mL 0.2420 mL 0.4841 mL 1.2102 mL
50 mM 0.0387 mL 0.1936 mL 0.3873 mL 0.9681 mL
60 mM 0.0323 mL 0.1614 mL 0.3227 mL 0.8068 mL
80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6051 mL
100 mM 0.0194 mL 0.0968 mL 0.1936 mL 0.4841 mL
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1,3-Dicaffeoylquinic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1,3-Dicaffeoylquinic acid19870-46-31,3-O-Dicaffeoylquinic acid 1,5-Dicaffeoylquinic acidPI3KAktReactive Oxygen SpeciesPhosphoinositide 3-kinasePKBProtein kinase BInhibitorinhibitorinhibit

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