2. MAPK/ERK Pathway Stem Cell/Wnt NF-κB
  3. ERK NF-κB
  4. 2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside

2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside  (Synonyms: 2,3,4',5-Tetrahydroxystilbene 2-O-D-glucoside)

Cat. No.: HY-N0652 Purity: 99.91%
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2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside isolats from the roots of Polygonaceae species, inhibits the formation of 5-HETE, HHT and thromboxane B2. 2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside has hypotensive, anti-ageing, anti-inflammatory, hypolipidemic, cardioprotective, and neuroprotective actions.

For research use only. We do not sell to patients.

2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside Chemical Structure

2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside Chemical Structure

CAS No. : 82373-94-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 32 In-stock
10 mg USD 50 In-stock
25 mg USD 100 In-stock
50 mg USD 140 In-stock
100 mg USD 190 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of 2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside:

2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside

WB

    2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside purchased from MedChemExpress. Usage Cited in: Molecules. 2020 Jul 6;25(13):3070.  [Abstract]

    Cytochrome c is analyzed by Western blotting in cytosolic and mitochondrial fractions. THSG treatment blocks the release of cytochrome c from mitochondria to the cytosol.

    View All ERK Isoform Specific Products:

    View All Isoforms
    ERK ERK1 ERK2 ERK5 ERK8

    View All NF-κB Isoform Specific Products:

    View All Isoforms
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside isolats from the roots of Polygonaceae species, inhibits the formation of 5-HETE, HHT and thromboxane B2. 2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside has hypotensive, anti-ageing, anti-inflammatory, hypolipidemic, cardioprotective, and neuroprotective actions[1][2].

    In Vitro

    2,3,4',5-tetrahydroxystilbene 2-O-D-glucoside (5-80 μM, 2 h) inhibits LPS-induced expression of pro-inflammatory cytokine (IL-6 and TNF-α) in RAW264.7 cells[3].
    2,3,4',5-tetrahydroxystilbene 2-O-D-glucoside (5-80 μM, 2 h) inhibits the expression of NO and mRNA levels of proinflammatory cytokine (IL-1β, IL-6, TNF-α and COX-2. ) in RAW264.7 cells[3].
    2,3,4',5-tetrahydroxystilbene 2-O-D-glucoside (5-80 μM, 2 h) suppresses NF-κB signaling pathway and ERK2 activation in LPS-induced RAW264.7 cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside Related Antibodies

    Western Blot Analysis[3]

    Cell Line: RAW264.7 cells
    Concentration: 5-80 μM
    Incubation Time: 2 h
    Result: Reduced the phosphorylation level of ERK2, IκBα, IKKβ, and NF-κB p65 in a dosedependent manner.
    Attenuated LPS-induced nuclear translocation with a dose-dependent behavior.
    In Vivo

    2,3,4',5-tetrahydroxystilbene 2-O-D-glucoside (30-60 mg/kg, i.p., daily, 7 days) can be used to cope with depressive-like symptoms in male ICR mice by inhibition of neuroinflammation and oxido-nitrosative stress[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male ICR mice[2]
    Dosage: 30-60 mg/kg
    Administration: i.p., daily, 7 days
    Result: Inhibited the production of proinflammatory cytokines induced by LPS, such as interleukin-1β, interleukin-6, and tumor necrosis factor-α.
    Prevented the LPS-induced enhancement of oxido-nitrosative stress in mouse hippocampus and prefrontal cortex.
    Prevented LPS-induced decreases in brain-derived neurotrophic factor levels in the hippocampus and prefrontal cortex.
    Molecular Weight

    406.39

    Formula

    C20H22O9
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    OC1=CC(O)=CC(/C=C/C2=CC=C(O)C=C2)=C1O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (246.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 10 mg/mL (24.61 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4607 mL 12.3035 mL 24.6069 mL
    5 mM 0.4921 mL 2.4607 mL 4.9214 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.12 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.12 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 16.67 mg/mL (41.02 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.91%

    COA (251 KB) HNMR (300 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.4607 mL 12.3035 mL 24.6069 mL 61.5173 mL
    5 mM 0.4921 mL 2.4607 mL 4.9214 mL 12.3035 mL
    10 mM 0.2461 mL 1.2303 mL 2.4607 mL 6.1517 mL
    15 mM 0.1640 mL 0.8202 mL 1.6405 mL 4.1012 mL
    20 mM 0.1230 mL 0.6152 mL 1.2303 mL 3.0759 mL
    DMSO 25 mM 0.0984 mL 0.4921 mL 0.9843 mL 2.4607 mL
    30 mM 0.0820 mL 0.4101 mL 0.8202 mL 2.0506 mL
    40 mM 0.0615 mL 0.3076 mL 0.6152 mL 1.5379 mL
    50 mM 0.0492 mL 0.2461 mL 0.4921 mL 1.2303 mL
    60 mM 0.0410 mL 0.2051 mL 0.4101 mL 1.0253 mL
    80 mM 0.0308 mL 0.1538 mL 0.3076 mL 0.7690 mL
    100 mM 0.0246 mL 0.1230 mL 0.2461 mL 0.6152 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside82373-94-22,3,4',5-Tetrahydroxystilbene 2-O-D-glucosideERKNF-κBExtracellular signal regulated kinasesNuclear factor-κBNuclear factor-kappaB5-HETEHHTthromboxane B2hypotensiveanti-ageinganti-inflammatoryhypolipidemiccardioprotectiveneuroprotectiveInhibitorinhibitorinhibit

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