3,5-Dihydroxybenzoic acid

Name: 3,5-Dihydroxybenzoic acid
Brand: MedChemExpress
SKU: HY-W015560
Rating: 4.5 (1 reviews)

Cat. No.: HY-W015560 Assay: 99.28%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3,5-Dihydroxybenzoic acid a potential biomarker for the consumption of many food products, including beer, nuts, peanut, and pulses.

For research use only. We do not sell to patients.

3,5-Dihydroxybenzoic acid Chemical Structure

CAS No. : 99-10-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 30	In-stock	Estimated Time of Arrival: December 31
5 g	USD 60	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 3,5-Dihydroxybenzoic acid:

3,5-Dihydroxybenzoic acid (Standard) Get quote

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Biological Activity
Purity & Documentation
Customer Review

Description

3,5-Dihydroxybenzoic acid a potential biomarker for the consumption of many food products, including beer, nuts, peanut, and pulses.

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	> 100 μM Compound: d	Inhibition of human recombinant PTP1B expressed in CHO cells assessed as p-nitorphenol production after 2 mins by microplate reader Inhibition of human recombinant PTP1B expressed in CHO cells assessed as p-nitorphenol production after 2 mins by microplate reader	[PMID: 21745700]
COS-7	EC₅₀	> 1000 μM Compound: 4	Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]
COS-7	EC₅₀	377 μM Compound: 4	Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]
RAW264.7	IC₅₀	117.11 μM Compound: 3b	Antagonist activity at TLR2 in mouse RAW264.7 cells assessed as PAM3CSK4-induced NO production measured after 24 hrs by Griess assay Antagonist activity at TLR2 in mouse RAW264.7 cells assessed as PAM3CSK4-induced NO production measured after 24 hrs by Griess assay	[PMID: 31255924]

Molecular Weight

154.12

Formula

C₇H₆O₄

CAS No.

99-10-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=CC(O)=CC(O)=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 60 mg/mL (389.31 mM; Need ultrasonic)

DMSO : 50 mg/mL (324.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.4885 mL	32.4423 mL	64.8845 mL
5 mM	1.2977 mL	6.4885 mL	12.9769 mL
10 mM	0.6488 mL	3.2442 mL	6.4885 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (324.42 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (244 KB) SDS (392 KB)

COA (248 KB) HNMR (181 KB) RP-HPLC (223 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	6.4885 mL	32.4423 mL	64.8845 mL	162.2113 mL
	5 mM	1.2977 mL	6.4885 mL	12.9769 mL	32.4423 mL
	10 mM	0.6488 mL	3.2442 mL	6.4885 mL	16.2211 mL
	15 mM	0.4326 mL	2.1628 mL	4.3256 mL	10.8141 mL
	20 mM	0.3244 mL	1.6221 mL	3.2442 mL	8.1106 mL
	25 mM	0.2595 mL	1.2977 mL	2.5954 mL	6.4885 mL
	30 mM	0.2163 mL	1.0814 mL	2.1628 mL	5.4070 mL
	40 mM	0.1622 mL	0.8111 mL	1.6221 mL	4.0553 mL
	50 mM	0.1298 mL	0.6488 mL	1.2977 mL	3.2442 mL
	60 mM	0.1081 mL	0.5407 mL	1.0814 mL	2.7035 mL
	80 mM	0.0811 mL	0.4055 mL	0.8111 mL	2.0276 mL
	100 mM	0.0649 mL	0.3244 mL	0.6488 mL	1.6221 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.