4-DAMP (Synonyms: 4-DAMP methiodide)

Name: 4-DAMP
Brand: MedChemExpress
SKU: HY-100958
Rating: 5.0 (2 reviews)

Cat. No.: HY-100958 Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-DAMP is a potent antagonist of M3 receptor and also has a high affinity for the closely-related M5 receptor.

For research use only. We do not sell to patients.

4-DAMP Chemical Structure

CAS No. : 1952-15-4

Size	Price	Stock	Quantity
5 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 255	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 4-DAMP

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Biological Activity
Purity & Documentation
References
Customer Review

Description

4-DAMP is a potent antagonist of M3 receptor and also has a high affinity for the closely-related M5 receptor^[1].

IC₅₀ & Target

mAChR3

mAChR5

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	3.2 x 10^-10 M Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	4.1 x 10^-10 M Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	4.9 x 10^-10 M Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO-K1	IC₅₀	0.055 μM Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	4.5 nM Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	5.1 nM Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]

Molecular Weight

451.34

Formula

C₂₁H₂₆INO₂

CAS No.

1952-15-4

Appearance

Solid

Color

White to off-white

SMILES

C[N+]1(C)CCC(OC(C(C2=CC=CC=C2)C3=CC=CC=C3)=O)CC1.[I-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (221.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2156 mL	11.0781 mL	22.1562 mL
5 mM	0.4431 mL	2.2156 mL	4.4312 mL
10 mM	0.2216 mL	1.1078 mL	2.2156 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.08 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (11.08 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (249 KB) HNMR (264 KB) RP-HPLC (260 KB) MS (68 KB) Elemental Analysis Report (108 KB)

Handling Instructions (2659 KB)

References

[1]. Watson N, et al. Comparative pharmacology of recombinant human M3 and M5 muscarinic receptors expressed in CHO-K1 cells. Br J Pharmacol. 1999 May;127(2):590-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2156 mL	11.0781 mL	22.1562 mL	55.3906 mL
	5 mM	0.4431 mL	2.2156 mL	4.4312 mL	11.0781 mL
	10 mM	0.2216 mL	1.1078 mL	2.2156 mL	5.5391 mL
	15 mM	0.1477 mL	0.7385 mL	1.4771 mL	3.6927 mL
	20 mM	0.1108 mL	0.5539 mL	1.1078 mL	2.7695 mL
	25 mM	0.0886 mL	0.4431 mL	0.8862 mL	2.2156 mL
	30 mM	0.0739 mL	0.3693 mL	0.7385 mL	1.8464 mL
	40 mM	0.0554 mL	0.2770 mL	0.5539 mL	1.3848 mL
	50 mM	0.0443 mL	0.2216 mL	0.4431 mL	1.1078 mL
	60 mM	0.0369 mL	0.1846 mL	0.3693 mL	0.9232 mL
	80 mM	0.0277 mL	0.1385 mL	0.2770 mL	0.6924 mL
	100 mM	0.0222 mL	0.1108 mL	0.2216 mL	0.5539 mL

4-DAMP Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-DAMP1952-15-44-DAMP methiodidemAChRMuscarinic acetylcholine receptorInhibitorinhibitorinhibit

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4-DAMP (Synonyms: 4-DAMP methiodide)

Customer Review

4-DAMP Related Antibodies

2 Publications Citing Use of MCE 4-DAMP

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

4-DAMP Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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