4-Hydroperoxy cyclophosphamide

Name: 4-Hydroperoxy cyclophosphamide
Brand: MedChemExpress
SKU: HY-117433
Rating: 5.0 (5 reviews)

Cat. No.: HY-117433 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Hydroperoxy cyclophosphamide is the active metabolite form of the precursor Cyclophosphamide. 4-Hydroperoxy cyclophosphamide cross-links DNA to induce T cell apoptosis independent of caspase receptor activation, and can activate the mitochondrial death pathway by producing reactive oxygen species (ROS). 4-Hydroperoxy cyclophosphamide can be used in the study of rheumatoid arthritis and autoimmune diseases.

For research use only. We do not sell to patients.

4-Hydroperoxy cyclophosphamide Chemical Structure

CAS No. : 39800-16-3

Size	Price	Stock	Quantity
1 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 171	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 304	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1190	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1960	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 4-Hydroperoxy cyclophosphamide:

4-Hydroperoxy Cyclophosphamide-d₄ Get quote

5 Publications Citing Use of MCE 4-Hydroperoxy cyclophosphamide

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•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
L1210	IC₅₀	90 μM Compound: 4-HC	Concentration required to reduce the viability of L1210 cells by 50% after 1-h incubation at 37 degrees C Concentration required to reduce the viability of L1210 cells by 50% after 1-h incubation at 37 degrees C	[PMID: 1992116]
V79	IC₅₀	2.7 μM Compound: 4-hydroperoxycyclophosphamide	Antiproliferative activity against NTR expressing chinese hamster V79 cells after 72 hrs Antiproliferative activity against NTR expressing chinese hamster V79 cells after 72 hrs	[PMID: 16821793]
V79	IC₅₀	3.1 μM Compound: 4-hydroperoxycyclophosphamide	Antiproliferative activity against chinese hamster V79 cells after 72 hrs Antiproliferative activity against chinese hamster V79 cells after 72 hrs	[PMID: 16821793]

In Vitro

4-Hydroperoxy cyclophosphamide (1 or 3 μg/mL, 24, 48, 72 h) Apoptosis of Caspase-independent T cells is mediated by oxidative stress-induced mitochondrial apoptosis factor AIF and nuclear relocalization of EndoG^[1].
4-Hydroperoxy cyclophosphamide (1 μg/mL, 72, 96 h) Combines with methotrexate (HY-14519), RANKL expression was inhibited in IL-6/ SIL-6R-induced fibroblast-like synoviocytes by inhibiting the JAK2/STAT3 and p38MAPK signaling pathways^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	CTL
Concentration:	3 μg/mL
Incubation Time:	24, 48, 72 h
Result:	Increased the expression of p53 and Bax after 24 h.

Apoptosis Analysis^[1]

Cell Line:	Jurkat, CTL
Concentration:	1 or 3 μg/mL
Incubation Time:	24 , 48 , 72h
Result:	Caspase-8 deficiency prevented CD95-mediated death induced by anti-CD95 monoclonal antibody (mAb) APO-1. Overexpression of Bcl-2 inhibited apoptosis. Induced cell death proceeded independently of caspase inhibition.

In Vivo

4-Hydroperoxy cyclophosphamide (200 mg/kg intraperitoneally injected) kills Caspase-independent T cells and B cells in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c mice^[1]
Dosage:	200 mg/kg
Administration:	i.p.
Result:	Exhibited a marked depletion of immature double-positive CD4 þ CD8 þ thymocytes and mature single-positive CD4⁺ and CD8⁺ T cells.

Molecular Weight

293.08

Formula

C₇H₁₅Cl₂N₂O₄P

CAS No.

39800-16-3

Appearance

Solid

Color

White to yellow

SMILES

O=P1(OCCC(N1)OO)N(CCCl)CCCl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (170.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4120 mL	17.0602 mL	34.1204 mL
5 mM	0.6824 mL	3.4120 mL	6.8241 mL
10 mM	0.3412 mL	1.7060 mL	3.4120 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.53 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (251 KB) Elemental Analysis Report (109 KB)

Handling Instructions (2659 KB)

References

[1]. Strauss G, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008 Feb;15(2):332-43. [Content Brief]

[2]. Niu HQ, et al. Combination of 4-hydroperoxy cyclophosphamide and methotrexate inhibits IL-6/sIL-6R-induced RANKL expression in fibroblast-like synoviocytes via suppression of the JAK2/STAT3 and p38MAPK signaling pathway. Int Immunopharmacol. 2018 Aug;61:45-53. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4120 mL	17.0602 mL	34.1204 mL	85.3009 mL
	5 mM	0.6824 mL	3.4120 mL	6.8241 mL	17.0602 mL
	10 mM	0.3412 mL	1.7060 mL	3.4120 mL	8.5301 mL
	15 mM	0.2275 mL	1.1373 mL	2.2747 mL	5.6867 mL
	20 mM	0.1706 mL	0.8530 mL	1.7060 mL	4.2650 mL
	25 mM	0.1365 mL	0.6824 mL	1.3648 mL	3.4120 mL
	30 mM	0.1137 mL	0.5687 mL	1.1373 mL	2.8434 mL
	40 mM	0.0853 mL	0.4265 mL	0.8530 mL	2.1325 mL
	50 mM	0.0682 mL	0.3412 mL	0.6824 mL	1.7060 mL
	60 mM	0.0569 mL	0.2843 mL	0.5687 mL	1.4217 mL
	80 mM	0.0427 mL	0.2133 mL	0.4265 mL	1.0663 mL
	100 mM	0.0341 mL	0.1706 mL	0.3412 mL	0.8530 mL