5,6,7-Trimethoxyflavone (Synonyms: Baicalein trimethyl ether)

Name: 5,6,7-Trimethoxyflavone
Brand: MedChemExpress
SKU: HY-110398
Rating: 4.5 (1 reviews)

Cat. No.: HY-110398 Purity: 98.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5,6,7-Trimethoxyflavone is a novel p38-α MAPK inhibitor with an anti-inflammatory effect. 5,6,7-Trimethoxyflavone is isolated from several plants including Zeyhera tuberculosa, Callicarpa japonica, and Kickxia lanigera.

For research use only. We do not sell to patients.

5,6,7-Trimethoxyflavone Chemical Structure

CAS No. : 973-67-1

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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View All Isoforms

p38 MAPK p38α p38β MAPK13 p38δ p38γ

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

p38-α MAPK^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	GI₅₀	53.99 μM Compound: 2	Growth inhibition of human HepG2 cells by MTT assay Growth inhibition of human HepG2 cells by MTT assay	[PMID: 27503682]
KB	IC₅₀	85.9 μM Compound: 6	Inhibitory activity against KB cell line after 72 h of drug exposure Inhibitory activity against KB cell line after 72 h of drug exposure	[PMID: 15481991]
KB 3-1	IC₅₀	57.9 μM Compound: 6	Inhibitory activity against KB/MDR cell line after 72 hr of drug exposure Inhibitory activity against KB/MDR cell line after 72 hr of drug exposure	[PMID: 15481991]
MCF7	GI₅₀	91.4 μM Compound: 2	Growth inhibition of human MCF7 cells by MTT assay Growth inhibition of human MCF7 cells by MTT assay	[PMID: 27503682]

Molecular Weight

312.32

Formula

C₁₈H₁₆O₅

CAS No.

973-67-1

Appearance

Solid

Color

White to light yellow

SMILES

O=C1C=C(C2=CC=CC=C2)OC3=CC(OC)=C(OC)C(OC)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (106.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2018 mL	16.0092 mL	32.0184 mL
5 mM	0.6404 mL	3.2018 mL	6.4037 mL
10 mM	0.3202 mL	1.6009 mL	3.2018 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.76%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (250 KB) HNMR (241 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Hassan AHE, et al. Repurposing mosloflavone/5,6,7-trimethoxyflavone-resveratrol hybrids: Discovery of novel p38-α MAPK inhibitors as potent interceptors of macrophage-dependent production of proinflammatory mediators. Eur J Med Chem. 2019 Jul 9;180:253-267. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2018 mL	16.0092 mL	32.0184 mL	80.0461 mL
	5 mM	0.6404 mL	3.2018 mL	6.4037 mL	16.0092 mL
	10 mM	0.3202 mL	1.6009 mL	3.2018 mL	8.0046 mL
	15 mM	0.2135 mL	1.0673 mL	2.1346 mL	5.3364 mL
	20 mM	0.1601 mL	0.8005 mL	1.6009 mL	4.0023 mL
	25 mM	0.1281 mL	0.6404 mL	1.2807 mL	3.2018 mL
	30 mM	0.1067 mL	0.5336 mL	1.0673 mL	2.6682 mL
	40 mM	0.0800 mL	0.4002 mL	0.8005 mL	2.0012 mL
	50 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6009 mL
	60 mM	0.0534 mL	0.2668 mL	0.5336 mL	1.3341 mL
	80 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0006 mL
	100 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL

5,6,7-Trimethoxyflavone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5,6,7-Trimethoxyflavone973-67-1Baicalein trimethyl etherp38 MAPKInhibitorinhibitorinhibit

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5,6,7-Trimethoxyflavone (Synonyms: Baicalein trimethyl ether)

Customer Review

5,6,7-Trimethoxyflavone Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All p38 MAPK Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

5,6,7-Trimethoxyflavone Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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