2. Epigenetics PI3K/Akt/mTOR Autophagy
  3. AMPK Autophagy
  4. 8-Chloroadenosine

8-Chloroadenosine  (Synonyms: 8-Cl-Ado)

Cat. No.: HY-103400 Purity: 99.97%
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8-Chloroadenosine (8-Cl-Ado), a unique ribonucleoside analog, depletes endogenous ATP that subsequently induces the phosphorylation and activation of AMPK. 8-Chloroadenosine induces autophagic cell death. 8-Chloroadenosine effectively inhibited in vivo tumor growth in mice.

For research use only. We do not sell to patients.

8-Chloroadenosine Chemical Structure

8-Chloroadenosine Chemical Structure

CAS No. : 34408-14-5

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1 mg USD 38 In-stock
5 mg USD 150 In-stock
10 mg USD 252 In-stock
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Based on 1 publication(s) in Google Scholar

8-Chloroadenosine Related Antibodies

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Description

8-Chloroadenosine (8-Cl-Ado), a unique ribonucleoside analog, depletes endogenous ATP that subsequently induces the phosphorylation and activation of AMPK. 8-Chloroadenosine induces autophagic cell death. 8-Chloroadenosine effectively inhibited in vivo tumor growth in mice[1].

IC50 & Target[1]

AMPK

 

In Vitro

8-Chloroadenosine (8-Cl-Ado; 10 μM; 24-72 hours) induces an autophagic flux in both LC3B-II and p62 levels[1].
8-Chloroadenosine (10 μM; 7-21 hours) induces phosphorylation of AMPK (Thr172) in a time-dependent manner in MCF-7 and BT-474 cells. Total AMPK protein levels were unchanged. 8-Chloroadenosine readily induces the phosphorylation of raptor Ser792 in MCF-7 cells. 8-Chloroadenosine (10 μM; 4-72 hours) induces ULK1 phosphorylation on Ser555 in MCF-7 and BT-474 cells[1].
8-Chloroadenosine attenuates mTOR activity in breast cancer cells. 8-Chloroadenosine is independent of p53[1].
8-Chloroadenosine (10 μM; 3 days) inhibits over 90% clonogenic survival in the MCF-7 breast cancer cell lines. The amount of apoptosis induction only reached ~30%[1].
8-Chloroadenosine (10 μM) treatment results in a rapid depletion of ATP within 12-hours in T47D, SK-BR-3, and ZR-75-1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8-Chloroadenosine Related Antibodies
In Vivo

8-Chloroadenosine (25-100 mg/kg; IP; three times a week for 3 weeks) inhibits growth of both MCF-7 and BT-474 xenograft tumors with 100 mg/kg. 8-Chloroadenosine with 50 mg/kg dose does not affect the growth of the BT-474 xenograft tumors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

301.69

Formula

C10H12ClN5O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2Cl)O[C@@H]1CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (414.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3147 mL 16.5733 mL 33.1466 mL
5 mM 0.6629 mL 3.3147 mL 6.6293 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.97%

COA (256 KB) HNMR (264 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3147 mL 16.5733 mL 33.1466 mL 82.8665 mL
5 mM 0.6629 mL 3.3147 mL 6.6293 mL 16.5733 mL
10 mM 0.3315 mL 1.6573 mL 3.3147 mL 8.2867 mL
15 mM 0.2210 mL 1.1049 mL 2.2098 mL 5.5244 mL
20 mM 0.1657 mL 0.8287 mL 1.6573 mL 4.1433 mL
25 mM 0.1326 mL 0.6629 mL 1.3259 mL 3.3147 mL
30 mM 0.1105 mL 0.5524 mL 1.1049 mL 2.7622 mL
40 mM 0.0829 mL 0.4143 mL 0.8287 mL 2.0717 mL
50 mM 0.0663 mL 0.3315 mL 0.6629 mL 1.6573 mL
60 mM 0.0552 mL 0.2762 mL 0.5524 mL 1.3811 mL
80 mM 0.0414 mL 0.2072 mL 0.4143 mL 1.0358 mL
100 mM 0.0331 mL 0.1657 mL 0.3315 mL 0.8287 mL
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8-Chloroadenosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Chloroadenosine34408-14-58-Cl-AdoAMPKAutophagyAMP-activated protein kinaseATPp-AMPKtranscriptionLC3B-IIp62MCF-7BT-474breast8-amino-ATPRNAInhibitorinhibitorinhibit

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