8-CPT-Cyclic AMP sodium (Synonyms: 8-CPT-cAMP sodium; 8-(p-Chlorophenylthio)-cAMP sodium)

Name: 8-CPT-Cyclic AMP sodium
Brand: MedChemExpress
SKU: HY-111673
Rating: 4.5 (1 reviews)

Cat. No.: HY-111673 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

8-CPT-Cyclic AMP (8-CPT-cAMP) sodium is a selective activator of cyclic AMP-dependent protein kinase (PKA). 8-CPT-Cyclic AMP sodium is also a potent inhibitor of the cyclic GMP-specific phosphodiesterase (PDE VA) with an IC₅₀ of 0.9 μM. 8-CPT-Cyclic AMP sodium also inhibits PDE III and PDE IV with IC₅₀Epac and is a potent Epac activator.

For research use only. We do not sell to patients.

8-CPT-Cyclic AMP sodium Chemical Structure

CAS No. : 93882-12-3

Size	Price	Stock	Quantity
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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PKA PKAR

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
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Description

In Vitro

a-Fas and TNF-α/CHX induces neutrophil death rapidly (within 2 h) to at least 90%. The commonly used cAMP analog 8-pCPT-cAMP (0.7 mM) delays TNF- /CHX-induced and a-Fas-induced apoptosis. It is more efficient against apoptosis induced by TNF- /CHX than against a-Fas^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

493.79

Formula

C₁₆H₁₄ClN₅NaO₆PS

CAS No.

93882-12-3

Appearance

Solid

Color

White to off-white

SMILES

NC1=NC=NC2=C1N=C(N2[C@@H]3O[C@@]4([H])[C@](OP(OC4)(O[Na])=O)([H])[C@H]3O)SC5=CC=C(Cl)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (126.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (101.26 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0252 mL	10.1258 mL	20.2515 mL
5 mM	0.4050 mL	2.0252 mL	4.0503 mL
10 mM	0.2025 mL	1.0126 mL	2.0252 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (255 KB) HNMR (279 KB) CNMR (327 KB) LCMS (89 KB)

Handling Instructions (2659 KB)

References

[1]. Connolly BJ, et al. 8-(4-Chlorophenyl)thio-cyclic AMP is a potent inhibitor of the cyclic GMP-specific phosphodiesterase (PDE VA). Biochem Pharmacol. 1992;44(12):2303-2306. [Content Brief]

[2]. Krakstad C, et al. cAMP protects neutrophils against TNF-alpha-induced apoptosis by activation of cAMP-dependent protein kinase, independently of exchange protein directly activated by cAMP (Epac). J Leukoc Biol. 2004;76(3):641-647. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0252 mL	10.1258 mL	20.2515 mL	50.6288 mL
	5 mM	0.4050 mL	2.0252 mL	4.0503 mL	10.1258 mL
	10 mM	0.2025 mL	1.0126 mL	2.0252 mL	5.0629 mL
	15 mM	0.1350 mL	0.6751 mL	1.3501 mL	3.3753 mL
	20 mM	0.1013 mL	0.5063 mL	1.0126 mL	2.5314 mL
	25 mM	0.0810 mL	0.4050 mL	0.8101 mL	2.0252 mL
	30 mM	0.0675 mL	0.3375 mL	0.6751 mL	1.6876 mL
	40 mM	0.0506 mL	0.2531 mL	0.5063 mL	1.2657 mL
	50 mM	0.0405 mL	0.2025 mL	0.4050 mL	1.0126 mL
	60 mM	0.0338 mL	0.1688 mL	0.3375 mL	0.8438 mL
	80 mM	0.0253 mL	0.1266 mL	0.2531 mL	0.6329 mL
	100 mM	0.0203 mL	0.1013 mL	0.2025 mL	0.5063 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

8-CPT-Cyclic AMP sodium Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-CPT-Cyclic AMP93882-12-38-CPT-cAMP8-(p-Chlorophenylthio)-cAMPPKAPhosphodiesterase (PDE)Protein kinase AcyclicAMPphosphodiesterasea-FasneutrophildeathInhibitorinhibitorinhibit

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8-CPT-Cyclic AMP sodium (Synonyms: 8-CPT-cAMP sodium; 8-(p-Chlorophenylthio)-cAMP sodium)

Customer Review

8-CPT-Cyclic AMP sodium Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKA Isoform Specific Products:

View All Phosphodiesterase (PDE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

8-CPT-Cyclic AMP sodium Related Antibodies

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Complete Stock Solution Preparation Table

8-CPT-Cyclic AMP sodium Related Classifications

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