AC-55541

Name: AC-55541
Brand: MedChemExpress
SKU: HY-14350
Rating: 4.5 (1 reviews)

Cat. No.: HY-14350 Purity: 99.25%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AC-55541 is a highly selective protease-activated receptor 2 (PAR2) agonist (pEC₅₀=6.7), displays no activity at other PAR subtypes or at over 30 other receptors involved in nociception and inflammation. AC-55541 has pEC₅₀ values of 5.9 and 6.6 in PI hydrolysis assays and Ca²⁺ mobilization assays and exhibits pronociceptive activity in vivo.

For research use only. We do not sell to patients.

AC-55541 Chemical Structure

CAS No. : 916170-19-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 103	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 103	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 143	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 257	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 386	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE AC-55541

•J Dermatol Sci. 2022 May 17;S0923-1811(22)00125-6. [Abstract]

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View All Protease Activated Receptor (PAR) Isoform Specific Products:

View All Isoforms

PAR1 PAR2 PAR4

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PAR2

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	EC₅₀	200 nM Compound: 10, AC-55541	Agonist activity at human PAR2 expressed in HEK293T cells assessed as stimulation of phosphatidylinositol hydrolysis after 45 mins by scintillation counting analysis Agonist activity at human PAR2 expressed in HEK293T cells assessed as stimulation of phosphatidylinositol hydrolysis after 45 mins by scintillation counting analysis	[PMID: 23895492]
HEK-293T	EC₅₀	200 nM Compound: 10, AC-55541	Agonist activity at human PAR2 expressed in HEK293T cells assessed as stimulation of cell proliferation after 5 days by beta-galactosidase assay Agonist activity at human PAR2 expressed in HEK293T cells assessed as stimulation of cell proliferation after 5 days by beta-galactosidase assay	[PMID: 23895492]
HEK-293T	EC₅₀	200 nM Compound: 10, AC-55541	Agonist activity at human PAR2 expressed in HEK293T cells assessed as increase in intracellular calcium level by bioluminescence assay Agonist activity at human PAR2 expressed in HEK293T cells assessed as increase in intracellular calcium level by bioluminescence assay	[PMID: 23895492]

In Vitro

AC-55541 (100 nM; 1 hour) suppresses poly I:C–induced CXCL10 mRNA in NHBE cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[2]

Cell Line:	NHBE cells
Concentration:	100 nM (prior to poly I:C stimulation)
Incubation Time:	1 hour
Result:	Poly I:C-induced CXCL10 mRNA was suppressed.

Molecular Weight

518.36

Formula

C₂₅H₂₀BrN₅O₃

CAS No.

916170-19-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC(C(C1=CC=CC=C12)=NNC2=O)C(N/N=C(C)/C3=CC=CC(Br)=C3)=O)C4=CC=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 51 mg/mL (98.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9292 mL	9.6458 mL	19.2916 mL
5 mM	0.3858 mL	1.9292 mL	3.8583 mL
10 mM	0.1929 mL	0.9646 mL	1.9292 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.82 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.25%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (254 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Gardell LR, et al. Identification and characterization of novel small-molecule protease-activated receptor 2 agonists. J Pharmacol Exp Ther. 2008 Dec;327(3):799-808. [Content Brief]

[2]. Homma T, et al. Role of Aspergillus fumigatus in Triggering Protease-Activated Receptor-2 in Airway Epithelial Cellsand Skewing the Cells toward a T-helper 2 Bias. Am J Respir Cell Mol Biol. 2016 Jan;54(1):60-70. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9292 mL	9.6458 mL	19.2916 mL	48.2290 mL
	5 mM	0.3858 mL	1.9292 mL	3.8583 mL	9.6458 mL
	10 mM	0.1929 mL	0.9646 mL	1.9292 mL	4.8229 mL
	15 mM	0.1286 mL	0.6431 mL	1.2861 mL	3.2153 mL
	20 mM	0.0965 mL	0.4823 mL	0.9646 mL	2.4115 mL
	25 mM	0.0772 mL	0.3858 mL	0.7717 mL	1.9292 mL
	30 mM	0.0643 mL	0.3215 mL	0.6431 mL	1.6076 mL
	40 mM	0.0482 mL	0.2411 mL	0.4823 mL	1.2057 mL
	50 mM	0.0386 mL	0.1929 mL	0.3858 mL	0.9646 mL
	60 mM	0.0322 mL	0.1608 mL	0.3215 mL	0.8038 mL
	80 mM	0.0241 mL	0.1206 mL	0.2411 mL	0.6029 mL

AC-55541 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AC-55541916170-19-9AC55541AC 55541Protease Activated Receptor (PAR)Thrombin receptorsInhibitorinhibitorinhibit

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AC-55541 Related Antibodies

1 Publications Citing Use of MCE AC-55541

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Protease Activated Receptor (PAR) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

AC-55541 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

AC-55541 Related Classifications

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