AI-10-49

Cat. No.: HY-16786 Purity: 99.24%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AI-10-49 is an inhibitor of leukemic oncoprotein CBFβ-SMHHC. AI-10-49 inhibits the binding of CBFβ-SMMHCto the RUNX1 Runt domain with IC₅₀ value of 0.26 μM. AI-10-49 can be used for the research of leukemia.

For research use only. We do not sell to patients.

AI-10-49 Chemical Structure

CAS No. : 1256094-72-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 166	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 166	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 114	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 348	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 588	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 792	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	0.19 μM Compound: AI-10-49	Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging	10.21203/rs.3.rs-23951/v1
Caco-2	CC₅₀	6.58 μM Compound: AI-10-49	Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay	10.21203/rs.3.rs-23951/v1

In Vitro

AI-10-49 inhibits the binding of CBFβ-SMMHCto theRUNX1 Runt domain with IC₅₀ value of 0.26 μM^[1].
? AI-10-49 (1 μM; 3, 6, 12 hours) has selectivity for inhibiting CBFβ-SMMHC binding to RUNX1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	ME-1 cells
Concentration:	1 μM
Incubation Time:	3, 6 hours
Result:	Effectively dissociated RUNX1 from CBFβ-SMMHC.

RT-PCR^[1]

Cell Line:	ME-1 and U937 cells
Concentration:	1 μM
Incubation Time:	6, 12 hours
Result:	Increased expression of RUNX3, CSF1R, and CEBPA.

In Vivo

AI-10-49 (200 mg/kg; per day) delays leukemia progression in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice (Cbfb^+/MYH11:Ras^+/G12D leukemic cells)^[1]
Dosage:	200 mg/kg
Administration:	per day
Result:	Reduced leukemia expansion in vivo and survived significantly long.

Molecular Weight

660.52

Formula

C₃₀H₂₂F₆N₆O₅

CAS No.

1256094-72-0

Appearance

Solid

Color

Off-white to yellow

SMILES

FC(F)(F)OC1=CC=C2N=C(C3=NC=C(OCCOCCOC4=CN=C(C5=NC6=CC=C(OC(F)(F)F)C=C6N5)C=C4)C=C3)NC2=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (51.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5140 mL	7.5698 mL	15.1396 mL
5 mM	0.3028 mL	1.5140 mL	3.0279 mL
10 mM	0.1514 mL	0.7570 mL	1.5140 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.24%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (245 KB) LCMS (137 KB)

Handling Instructions (2659 KB)

References

[1]. Anuradha Illendula, et al. Chemical biology. A small-molecule inhibitor of the aberrant transcription factor CBFβ-SMMHC delays leukemia in mice. Science. 2015 Feb 13;347(6223):779-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5140 mL	7.5698 mL	15.1396 mL	37.8490 mL
	5 mM	0.3028 mL	1.5140 mL	3.0279 mL	7.5698 mL
	10 mM	0.1514 mL	0.7570 mL	1.5140 mL	3.7849 mL
	15 mM	0.1009 mL	0.5047 mL	1.0093 mL	2.5233 mL
	20 mM	0.0757 mL	0.3785 mL	0.7570 mL	1.8924 mL
	25 mM	0.0606 mL	0.3028 mL	0.6056 mL	1.5140 mL
	30 mM	0.0505 mL	0.2523 mL	0.5047 mL	1.2616 mL
	40 mM	0.0378 mL	0.1892 mL	0.3785 mL	0.9462 mL
	50 mM	0.0303 mL	0.1514 mL	0.3028 mL	0.7570 mL

AI-10-49 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AI-10-491256094-72-0OthersCBFB-SMMHC fusion proteinacute myeloid leukemia (AML)bindingRUNX1 Runt domainInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks