AMI-1

Name: AMI-1
Brand: MedChemExpress
SKU: HY-18962
Rating: 5.0 (6 reviews)

Cat. No.: HY-18962 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AMI-1 is a potent, cell-permeable and reversible inhibitor of protein arginine N-methyltransferases (PRMTs), with IC₅₀s of 8.8 μM and 3.0 μM for human PRMT1 and yeast-Hmt1p, respectively. AMI-1 exerts PRMTs inhibitory effects by blocking peptide-substrate binding.

For research use only. We do not sell to patients.

AMI-1 Chemical Structure

CAS No. : 20324-87-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of AMI-1:

AMI-1 free acid Get quote

5 Publications Citing Use of MCE AMI-1

: AMI-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Feb 23;14(1):1011. [Abstract]; AMI-1 or adenosine dialdehyde (AdOx) markedly reduces the arginine methylation of PHGDH in HEK293T, Huh7, and PLC/PRF/5 cells.

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View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms

EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PRMT1

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human A375 cells by MTT assay Cytotoxicity against human A375 cells by MTT assay	[PMID: 26428871]
Daoy	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human DaOY cells assessed as reduction in cell viability after 72 hrs by CellTiter-blue assay Cytotoxicity against human DaOY cells assessed as reduction in cell viability after 72 hrs by CellTiter-blue assay	[PMID: 28927791]
DLD-1	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human DLD1 cells by MTT assay Cytotoxicity against human DLD1 cells by MTT assay	[PMID: 26428871]
HepG2	IC₅₀	51.17 μM Compound: AMI-1	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by CellTiter-blue assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by CellTiter-blue assay	[PMID: 28927791]
MCF7	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by CellTiter-blue assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by CellTiter-blue assay	[PMID: 28927791]
MCF7	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 26428871]
SH-SY5Y	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human SH-SY5Y cells by MTT assay Cytotoxicity against human SH-SY5Y cells by MTT assay	[PMID: 26428871]
T-24	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human T24 cells by MTT assay Cytotoxicity against human T24 cells by MTT assay	[PMID: 26428871]
UMUC3	IC₅₀	> 100 μM Compound: AMI-1	Cytotoxicity against human UM-UC-3 cells by MTT assay Cytotoxicity against human UM-UC-3 cells by MTT assay	[PMID: 26428871]

In Vitro

AMI-1 can inhibit the in vitro methylation reactions performed by all five recombinantly active PRMTs (PRMT1, -3, -4, and -6 and Hmt1p)^[2].
? AMI-1 not only inhibits type I PRMTs (PRMT1, 3, 4 and 6) but also type II PRMT5^[2].
? AMI-1 specifically inhibits arginine, but not lysine, methyltransferase activity in vitro and does not compete for the AdoMet binding site^[3].
? AMI-1 inhibits methylation of GFP-Npl3 and cellular proteins^[3].
? AMI-1 (0.6-2.4 mM; 48-96 hours) inhibits the cell viability of sarcoma in S180 and U2OS cells in a time-dependent and dose-dependent manner in vitro^[4].
? AMI-1 (1.2-2.4 mM; 48-72 hours) reduces S180 cell viability through the induction of cell apoptosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	S180 cells, U2OS cells
Concentration:	0.6 mM, 1.2 mM, 2.4 mM
Incubation Time:	48 hours, 72 hours, 96 hours
Result:	Inhibited the cell viability.

Apoptosis Analysis^[4]

Cell Line:	S180 cells
Concentration:	1.2 mM, 2.4 mM
Incubation Time:	48 hours, 72 hours
Result:	Increased the percentages of cells undergoing apoptosis.

In Vivo

AMI-1 (0.5 mg; intratumorally; daily; for 7 days) inhibits S180 viability in vivo^[4].
? AMI-1 (0.5 mg; intratumorally; daily; for 7 days) downregulates PRMT5 but does not regulate the expression of PRMT7 in a tumor xenograft model^[4].
? AMI-1 (0.5 mg; intratumorally; daily; for 7 days) decreases the levels of H4R3me2s and H3R8me2s in a tumor xenograft model^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-7 weeks old male Kunming mice (18-22 g), with S180 cells xenograft^[4]
Dosage:	0.5 mg
Administration:	Intratumorally, daily, for 7 days
Result:	Decreased tumor weight.

Molecular Weight

548.45

Formula

C₂₁H₁₄N₂Na₂O₉S₂

CAS No.

20324-87-2

Appearance

Solid

Color

Light brown to brown

SMILES

O=C(NC1=CC2=CC(S(=O)(O[Na])=O)=CC(O)=C2C=C1)NC3=CC4=CC(S(=O)(O[Na])=O)=CC(O)=C4C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 62.5 mg/mL (113.96 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8233 mL	9.1166 mL	18.2332 mL
5 mM	0.3647 mL	1.8233 mL	3.6466 mL
10 mM	0.1823 mL	0.9117 mL	1.8233 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (182.33 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (247 KB) HNMR (187 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang, B., et al. Targeting protein arginine methyltransferase 5 inhibits colorectal cancer growth by decreasing arginine methylation of eIF4E and FGFR3. Oncotarget. 2015 Sep 8;6(26):22799-811. [Content Brief]

[2]. Baolai Zhang, et al. Arginine Methyltransferase inhibitor-1 Inhibits Sarcoma Viability in vitro and in vivo. Oncol Lett. 2018 Aug;16(2):2161-2166. [Content Brief]

[3]. Donghang Cheng, et al. Small Molecule Regulators of Protein Arginine Methyltransferases. J Biol Chem. 2004 Jun 4;279(23):23892-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.8233 mL	9.1166 mL	18.2332 mL	45.5830 mL
	5 mM	0.3647 mL	1.8233 mL	3.6466 mL	9.1166 mL
	10 mM	0.1823 mL	0.9117 mL	1.8233 mL	4.5583 mL
	15 mM	0.1216 mL	0.6078 mL	1.2155 mL	3.0389 mL
	20 mM	0.0912 mL	0.4558 mL	0.9117 mL	2.2792 mL
	25 mM	0.0729 mL	0.3647 mL	0.7293 mL	1.8233 mL
	30 mM	0.0608 mL	0.3039 mL	0.6078 mL	1.5194 mL
	40 mM	0.0456 mL	0.2279 mL	0.4558 mL	1.1396 mL
	50 mM	0.0365 mL	0.1823 mL	0.3647 mL	0.9117 mL
	60 mM	0.0304 mL	0.1519 mL	0.3039 mL	0.7597 mL
	80 mM	0.0228 mL	0.1140 mL	0.2279 mL	0.5698 mL
	100 mM	0.0182 mL	0.0912 mL	0.1823 mL	0.4558 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

AMI-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMI-120324-87-2AMI1AMI 1Histone Methyltransferaseprotein arginine N-methyltransferasesPRMTsyeast-Hmt1pPRMT1Hmt1pInhibitorinhibitorinhibit

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AMI-1

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Other Forms of AMI-1:

AMI-1 Related Antibodies

5 Publications Citing Use of MCE AMI-1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Methyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

AMI-1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AMI-1 Related Classifications

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